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Method for extracting high-purity beta-glucosan from oat bran

A technology of oat bran and oat bran powder, which is applied in the field of extracting high-purity β-glucan, which can solve the problems of single purity and yield, neglect of influence, and low purity

Inactive Publication Date: 2014-05-07
常州毅博生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The purity and relative molecular weight of oat β-glucan are the main factors affecting its biological activity. The purity of oat β-glucan obtained by the current extraction method is relatively low, and it is only a single pursuit of purity and yield, ignoring the impact of molecular weight on Effect of β-glucan application

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] 1. Enzyme inactivation: add oat bran powder to 10 times the volume of 80% (v / v) ethanol solution, reflux at 95°C to inactivate enzyme; filter after cooling, and dry the filter residue;

[0033] 2. Extraction: Extract β-glucan with hot water under the conditions of material-to-water ratio 1:10, pH 7.0, 60°C, and extraction time 4 hours;

[0034] 3. Enzymolysis: After extraction, the supernatant was collected by centrifugation and added to enzymolysis with amylase. The activity of amylase was 480KNU-B / g, and the amount added was 0.1% (dry basis). React at 60°C for 0.5h to remove the starch, which is degraded into small molecular oligosaccharides;

[0035] 4. Isoelectric point precipitation: cooling to 25°C, adjusting the pH to 5.0, stirring and standing still, precipitation to remove protein;

[0036] 5. Alcohol precipitation: Centrifuge to obtain the supernatant, adjust the pH to 7.0, add ethanol to a concentration of 50% (v / v) under high-speed stirring, and let stand; ...

Embodiment 2

[0042] 1. Enzyme inactivation: add oat bran powder to 15 times the volume of 80% (v / v) ethanol solution, reflux at 95°C to inactivate enzyme; filter after cooling, and dry the filter residue;

[0043] 2. Extraction: Extract β-glucan with hot water under the conditions of material-to-water ratio 1:14, pH 5.0, 60°C, and extraction time 3 hours;

[0044] 3. Enzymolysis: After extraction, the supernatant was collected by centrifugation and added to amylase for enzymolysis. The activity of amylase was 480KNU-B / g, and the addition amount was 0.1% (dry basis). React at 60°C for 1 hour to remove the amylase in the filtrate. Starch, which is degraded into small molecule oligosaccharides;

[0045] 4. Isoelectric point precipitation: cooling to 25°C, adjusting the pH to 4.5, stirring and standing still, precipitation to remove protein;

[0046] 5. Alcohol precipitation: Centrifuge to obtain the supernatant, adjust the pH to 7.0, add ethanol to a concentration of 50% (v / v) under high-spe...

Embodiment 3

[0052] 1. Enzyme inactivation: add oat bran powder to 18 times the volume of 75% (v / v) ethanol solution, reflux at 95°C to inactivate enzyme; filter after cooling, and dry the filter residue;

[0053] 2. Extraction: Extract β-glucan with hot water under the conditions of material-to-water ratio 1:17, pH 6.0, 68°C, and extraction time 2 hours;

[0054] 3. Enzymolysis: After extraction, the supernatant was collected by centrifugation and added to amylase for enzymolysis. The activity of amylase was 480KNU-B / g, and the addition amount was 0.2% (dry basis). React at 60°C for 1 hour to remove the amylase in the filtrate. Starch, which is degraded into small molecule oligosaccharides;

[0055] 4. Isoelectric point precipitation: cooling to 25°C, adjusting the pH to 5.0, stirring and standing still, precipitation to remove protein;

[0056] 5. Alcohol precipitation: Centrifuge to obtain the supernatant, adjust the pH to 6.5, add ethanol to a concentration of 50% (v / v) under high-spe...

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Abstract

The invention discloses a method for extracting high-purity beta-glucosan from oat bran. According to the method, oat bran powder is sequentially treated by adopting the following steps: (1) carrying out ethyl alcohol reflux and enzyme deactivation; (2) stirring and extracting in hot water; (3) centrifuging and collecting supernatant; (4) carrying out enzymolysis and isoelectric precipitation; (5) carrying out alcohol precipitation; (6) centrifuging, collecting and precipitating; (7) carrying out ammonium sulfate precipitation; (8) dialyzing and removing impurities; and (9) carrying out freeze drying, so that the oat beta-glucosan is obtained. The high-purity oat beta-glucosan is obtained by adopting a mild water extraction method through extraction and treatment steps such as enzymolysis, isoelectric precipitation, alcohol precipitation, ammonium sulfate precipitation and dialysis. The high-purity oat beta-glucosan has a certain antioxidation effect on oil and can be added to yoghourt so as to improve the texture characteristics and nutritional ingredients of the yoghourt.

Description

Technical field: [0001] The invention relates to food processing and treatment, in particular to a method for extracting high-purity beta-glucan from oat bran. Background technique: [0002] As we all know, the extraction and purification of oat β-glucan should keep its original structure and biological activity as much as possible, and pay attention to its yield and purity. The oat β-glucan is soluble in water, acid, alkali, and salt solution, but insoluble in organic solvents such as alcohol, ether, and ketone for extraction. At present, the raw materials are generally inactivated and degreased, and then classified according to the difference in molecular size and shape, or according to the different properties of the groups carried by the molecules, and finally separated by step-by-step precipitation. Since the obtained crude extract is not a pure compound, but also contains impurities such as protein, starch, and other polysaccharides, it is necessary to add a precipita...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/02
Inventor 黄龙陈佳李燕
Owner 常州毅博生物科技有限公司
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