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Method of detecting urotropin in main stream smoke of cigarette

A technology for mainstream cigarette smoke and urotropine, which is applied in the field of cigarette smoke and achieves the effects of accurate quantification, high detection sensitivity and easy operation

Active Publication Date: 2015-05-13
CHINA TOBACCO JIANGSU INDAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As the public pays more and more attention to smoking and health issues, harmful components in cigarette smoke are getting more and more attention. However, there are relatively few relevant reports on the detection of urotropine in cigarette smoke. In view of this, it is necessary to invent a method for detecting Method of urotropine in mainstream smoke

Method used

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  • Method of detecting urotropin in main stream smoke of cigarette
  • Method of detecting urotropin in main stream smoke of cigarette
  • Method of detecting urotropin in main stream smoke of cigarette

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] A method for detecting urotropine in cigarette mainstream smoke, comprising the following steps:

[0021] 1) Solution preparation

[0022] Preparation of extraction solution: accurately add 4 ml of triethylamine to 1 L of ethanol solution.

[0023] Preparation of standard stock solution: Accurately prepare 2 mg / L standard stock solution of urotropine with ethanol.

[0024] Preparation of internal standard solution: Accurately prepare 100 μg / L and 1 mg / L internal standard solutions of methyl phenylacetate with ethanol.

[0025] 2) Sample testing

[0026] According to the provisions of ISO4387:2000, capture the total particulate matter of the mainstream smoke of 20 cigarettes, put the Cambridge filter disc that has captured the total particulate matter of the mainstream smoke into a 250mL bread bottle, add 40mL of ethanol containing triethylamine Shake the extract to extract the Cambridge filter for 40 minutes, accurately pipette 20mL of the extract to pass through the...

Embodiment 2

[0032] According to the method of Example 1, the lowest concentration of the standard solution was continuously injected 10 times, and the standard deviation was measured, with 3 times the standard deviation as the detection limit and 10 times the standard deviation as the limit of quantification. The results are shown in Table 1. The detection limit of the product was 0.32 ng / cig, and the lower limit of quantification was 1.06 ng / cig. The content of urotropine in flue-cured cigarette smoke is generally around 30 ng / cig, so this method can meet the analysis and determination of cigarette samples.

[0033] Table 1 Analysis of the results of 10 injections of the lowest standard sample

[0034]

Embodiment 3

[0036] According to the method of embodiment 1, select a certain flue-cured tobacco type cigarette sample, measure 4 times every day simultaneously, measure 3 days altogether, calculate the intraday repeatability and daytime reproducibility of the assay method, the result is shown in table 2 relative standard deviation (% ) were 4.37, 3.89 and 2.85 respectively, and the relative standard deviation (%) between days was 3.58, so the precision of this method is good.

[0037] Table 2 Precision analysis results of the method (unit: ng / cig)

[0038]

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Abstract

The invention discloses a method of detecting urotropin in main stream smoke of cigarette. The method comprises the following steps: capturing the main stream smoke of cigarette by using a cambridge filter; extracting the cambridge filter with alkaline ethyl alcohol under oscillation; enabling extract liquor to pass through an activated silica gel column; leaching the column with dichloromethane, discarding the leacheate and then leaching the column with methyl alcohol, collecting the leacheate and then concentrating, adding an interior label solution, detecting by GC-MS, and analyzing the content of the urotropin in side stream smoke of cigarette. According to the invention, the content of the urotropin in main stream smoke of cigarette is determined by the GC-MS for the first time. The method has the characteristics of being simple and convenient to operate, accurate to quantify and high in detection sensitivity; the detection limit is 0.32 ng / cig, the quantifying lower limit is 1.06 ng / cig, the labeled recycling rate is 91.10-108.73%, the intraday relative standard deviation is 2.85-4.37%, the diurnal relative standard deviation is 3.58%, and the method is suitable for determination of urotropin in main stream smokes of various cigarettes.

Description

technical field [0001] The invention belongs to the technical field of cigarette smoke, and in particular relates to a method for detecting urotropine in mainstream smoke of cigarettes. Background technique [0002] Urotropine is a harmful chemical component, corrosive, and can cause burns to the human body. Its melting point is 263°C. If it exceeds this melting point, it will sublimate and decompose, but it will not melt. When the temperature rises to 300°C, hydrogen cyanide is released, and when the temperature rises again, it decomposes into methane, hydrogen and nitrogen. When the human body contacts the product, it mainly causes dermatitis and eczema. Hexatropine is distributed in the mainstream and side stream smoke of cigarettes. Hexaamine in the smoke is a secondary product of the smoke, which is produced by the reaction of ammonia and formaldehyde in the smoke. The side stream smoke The content in it is higher than that in mainstream smoke. As the public pays mor...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02
Inventor 沈晓晨庄亚东张华王晨辉王珂清刘献军石怀彬曹毅朱莹张媛尤晓娟朱龙杰
Owner CHINA TOBACCO JIANGSU INDAL