Fermentation stock seed for liquid culture of morchella esculenta, preparation method of fermentation stock seed and method for realizing liquid culture by using stock seed

A liquid culture, liquid fermentation technology, applied in the application, fertilizer mixture, fertilization device and other directions, can solve the problems such as no commercial artificial cultivation of Morchella, air sensitivity, etc., to shorten the fermentation period, the mycelium grows fast, and the fermentation The effect of cycle shortening

Inactive Publication Date: 2014-05-21
GANSU ACAD OF SCI INST OF BIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Although there are reports that the artificial cultivation of Morchella has been realized, so far there is no repo

Method used

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  • Fermentation stock seed for liquid culture of morchella esculenta, preparation method of fermentation stock seed and method for realizing liquid culture by using stock seed
  • Fermentation stock seed for liquid culture of morchella esculenta, preparation method of fermentation stock seed and method for realizing liquid culture by using stock seed
  • Fermentation stock seed for liquid culture of morchella esculenta, preparation method of fermentation stock seed and method for realizing liquid culture by using stock seed

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Example Embodiment

[0024] Example 1. The preparation method of Morchella liquid culture fermentation stock is as follows:

[0025] A. Preparation of first-level species: Morchella sp. 801 was purchased from Mianyang Institute of Edible Fungi, Sichuan;

[0026] B. Morel liquid culture fermentation stock culture medium composition: sawdust 2000g, wheat bran 7000g, corn flour 500g, soybean flour 460g, dipotassium phosphate 15g, potassium dihydrogen phosphate 15g, magnesium sulfate 10g, vitamin B 1 100mg, vitamin B 2 100 mg, 12.5L water;

[0027] C. Cultivation of morel liquid culture fermentation stock: mix well the stock culture medium of step B and put them into 500ml polypropylene plastic bags of edible fungi with suitable tightness. Seal with cotton plug and sterilize with high pressure 0.12MPa for 1 hour. . After cooling, the aseptic operation connects to the morel species prepared in step A, 25 o C culture at a constant temperature for 20 days can get morel liquid culture stock.

Example Embodiment

[0028] Example 2. The preparation method of Morchella liquid culture fermentation stock is as follows:

[0029] A. Preparation of first-level species: Morchella sp. 801 was purchased from Mianyang Institute of Edible Fungi, Sichuan;

[0030] B. Morel liquid culture fermentation stock culture medium composition: sawdust 2500g, wheat bran 6500g, corn flour 600g, soy flour 370g, dipotassium phosphate 10g, potassium dihydrogen phosphate 10g, magnesium sulfate 20g, vitamin B 1 100mg, vitamin B 2 150mg, 12.0L of water.

[0031] C. Cultivation of morel liquid culture fermentation stock: mix well the stock culture medium of step B and put them into 500ml polypropylene plastic bags of edible fungi with suitable tightness. Seal with cotton plug and sterilize with high pressure 0.12MPa for 1 hour. . After cooling, the aseptic operation is connected to the morel species prepared in step A, 23 o C culture at a constant temperature for 20 days can get morel liquid culture stock.

Example Embodiment

[0032] Example 3. The preparation method of the morel liquid culture fermentation stock is as follows:

[0033] A. Preparation of first-level species: Morchella sp. 801 was purchased from Mianyang Institute of Edible Fungi, Sichuan;

[0034] B. Morel liquid culture fermentation stock culture medium composition: sawdust 1500g, wheat bran 7500g, corn flour 450g, soybean flour 500g, dipotassium hydrogen phosphate 20g, potassium dihydrogen phosphate 25g, magnesium sulfate 15g, vitamin B 1 150mg, vitamin B 2 100 mg, 14.0L of water.

[0035] C. Cultivation of morel liquid culture fermentation stock: mix well the stock culture medium of step B and put them into 500ml polypropylene plastic bags of edible fungi with suitable tightness. Seal with cotton plug and sterilize with high pressure 0.12MPa for 1 hour. . After cooling, the aseptic operation is connected to the morel species prepared in step A, 27 o C culture at a constant temperature for 20 days can get morel liquid culture stock.

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Abstract

The invention discloses a fermentation stock seed for the liquid culture of morchella esculenta, a preparation method of the fermentation stock seed and a method for realizing liquid culture by using the stock seed. The preparation method comprises the following steps: preparing a solid culture medium of morchella esculenta by taking sawdust, wheat bran, corn meal and soybean meal as raw materials; carrying out inoculated culture to form the fermentation stock seed for the liquid culture of the morchella esculenta. The solid fermentation stock seed is not needed to be expanded step by step and can be directly used for the liquid culture of the morchella esculenta; the preparation method is simple in operation and rapid in mycelial growth; compared with the traditional process, the preparation method has the advantages of shortening the fermentation period and remarkably increasing the content of exopolysaccharide in the morchella esculenta.

Description

Technical field [0001] The invention is the field of bacterial cultivation, which involves a method of cultivating the original species and preparation methods of the cultivation of a liquid in the cultivation of liquid cultivation. Background technique [0002] Lammid is a precious and rare wild food bacteria with high nutritional value.Modern pharmacological studies have shown that the effective component of the biological activity of the diseased biological activity (medicine) has a high content of fungal polysaccharides, and its sub -entity polysaccharides has the effect of eliminating insecticidal and insecticidal.Tumor effects can reduce cholesterol content and prevent arteriosclerosis. [0003] At present, although there are reports of artificial cultivation of more thanhistan, there have been reports that have not seen commercialized artificial cultivation of sheep. The main reason is that the formation stage of more than the formation stage of the formation of more sensi...

Claims

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Application Information

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IPC IPC(8): C05G3/00
Inventor 韩融冰路等学赵玉卉王龙
Owner GANSU ACAD OF SCI INST OF BIOLOGY
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