Preparation method of deep-sea fishskin collagen polypeptide

Abstract

The invention discloses a preparation method of deep-sea fishskin collagen polypeptide. The method comprises the following steps: adding a crude collagen protein liquid into a compound proteinase to carry out enzymolysis at the temperature of 55 DEG C; filtering many times by using an ultrafiltration membrane in the enzymolysis process; then, after decoloring and deodorizing the enzymatic hydrolysate by using composite activated carbon by virtue of a multi-step adsorption method, carrying out nanofiltration and drying to obtain the powdery collagen polypeptide. According to the invention, an enzymolysis and ultrafiltration separation combined process is adopted to truly realize the artificial control for reaction conditions, so that the molecular weight of the deep-sea fishskin collagen polypeptide is controllable and is reasonably distributed, and furthermore, various physicochemical properties of the deep-sea fishskin collagen polypeptide are better.

Description

technical field [0001] The invention belongs to the technical field of enzymatic hydrolysis of marine organisms, and in particular relates to a method for preparing collagen polypeptides by enzymatic hydrolysis using deep-sea fish skin collagen liquid as a substrate. Background technique [0002] Protein is one of the essential substances for the growth and development of the human body and the maintenance of normal life activities. Modern biological metabolism studies have shown that after protein digestion, it is mainly absorbed directly in the form of polypeptides. For example, fish skin collagen polypeptide not only has the same amino acid composition as fish skin protein, but also has better digestion and absorption than protein state. Certain low-peptides can not only provide the nutrients needed for human growth and development, but also have the functions of preventing and treating diseases and regulating the physiological functions of the human body. Some hydrolyz...

AI Technical Summary

Problems solved by technology

[0005] Existing enzymatic hydrolysis technologies focus on controlling the initial reaction conditions such as substrate concentration, temperature, pH, and enzyme dosage. However, as the enzymolysis reaction proceeds, the substrate concentration and pH change, resulting in collagen polypeptide enzymes The hydrolysis process has complex, cumbersome, and difficult-to-control problems. Therefore, it is difficult to effectively control the changes in these conditions during the reaction process in the existing technology. production of bitter peptides

[0006] The traditional process of decolorization and deodorization uses a single activated carbon, but the treatment effect of the product is not good, and other auxiliary treatment processes such as hydrogen peroxide and other oxidants are needed for oxidative decolorization

Because activated carbon is made of different raw materials and preparation processes, each activated carbon has its own adsorption characteristics and adsorption selectivity: adsorption of specific impurities and adsorption conditions: temperature, pH, solution concentration, reaction time, etc., the above Any unreasonable control of the action conditions can lead to poor treatment effect of the product, so the product must be treated with composite activated carbon

[0007] In addition, the existing collagen peptide products have a common problem: the molecular weight distribution is unreasonable, and the physicochemical and functional properties of collagen peptides are closely related to their molecular weight distribution and amino acid composition.

The part below 500 Daltons is composed of oligopeptides and amino acids, the biological activity of this part is poor, and most of them are bitter peptides

Images