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Micro-fluidic chip system and method for pesticide residue field detection

A pesticide residue detection, microfluidic chip technology, applied in the direction of analytical materials, instruments, etc., can solve the problems of strong subjectivity of detection results, poor detection sensitivity and accuracy, poor detection sensitivity and accuracy, etc., to improve the detection portability. The effect of the degree of automation and

Inactive Publication Date: 2014-05-21
HANGZHOU TINKER BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing rapid detection cards for pesticide residues are based on the enzyme inhibition reaction on solid-phase carriers such as test paper to realize the detection of pesticide residues. It belongs to a "dry chemical method" biochemical reaction. Difference
In addition, the current detection of pesticide residue quick test cards relies entirely on human interpretation. As the sensitivity and accuracy of inspectors to the color changes of quick test cards vary widely, the detection results of pesticide residue quick test cards are highly subjective, sensitive and accurate. rate difference

Method used

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  • Micro-fluidic chip system and method for pesticide residue field detection
  • Micro-fluidic chip system and method for pesticide residue field detection
  • Micro-fluidic chip system and method for pesticide residue field detection

Examples

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Effect test

Embodiment 1

[0032] Example 1 A microfluidic chip system for on-site rapid detection of pesticide residues

[0033] In this example, the microfluidic chip system for on-site rapid detection of pesticide residues includes a microfluidic chip and a portable instrument ( figure 1 ), where the structure and operation process of the microfluidic chip are as follows image 3 As shown, the microfluidic chip is composed of a sample inlet a1, a fluid channel b1, an enzyme inhibition reaction pool c1, a color reaction and detection pool d1, and a fluid outlet e1; f1 is the mixture of enzyme and color reagent stored in the reaction pool Reagent, g1 is the substrate stored in the detection pool, h1 is the sample extract. The specific detection process is as follows: ①Sampling: Quantitative sample solution h1 (such as fruit, vegetable extract or water quality sample) is quantitatively injected into the microfluidic chip from the injection port a1 along the fluid channel b1, and Fill the enzyme inhibi...

Embodiment 2

[0034] Example 2 A microfluidic chip system for on-site rapid detection of pesticide residues

[0035] In this example, the microfluidic chip system for on-site rapid detection of pesticide residues includes a microfluidic chip and a portable instrument ( figure 1 ), where the structure and operation process of the microfluidic chip are as follows Figure 4 As shown, the microfluidic chip is composed of a sample inlet a2, a fluid channel b2, an enzyme inhibition reaction pool c2, a color reaction pool d2, a detection pool d2', and a fluid outlet e2; f2 is the enzyme stored in the reaction pool and Chromogenic reagent mixed reagent, g2 is the substrate stored in the chromogenic reaction pool, h2 is the sample extract. The specific detection process is: ①Sampling: Quantitative sample solution h2 to be tested (such as fruit, vegetable extract or water quality sample) is quantitatively injected into the microfluidic chip from the injection port a2 along the fluid channel b2, and ...

Embodiment 3

[0036] In this example, the microfluidic chip system for on-site rapid detection of pesticide residues includes a microfluidic chip and a portable instrument ( figure 1 ), where the structure and operation process of the microfluidic chip are as follows Figure 5 As shown, the microfluidic chip is composed of the sample inlet and sample pretreatment pool a3, the fluid channel b3, the enzyme inhibition reaction pool c3, the color reaction and detection pool d3, and the fluid outlet e3; f3 is the enzyme stored in the reaction pool The reagent is mixed with the chromogenic reagent, g3 is the substrate stored in the chromogenic reaction pool, and h3 is the sample and extract. The specific detection process is as follows: ①Sampling: Add a certain quality of the sample to be tested (such as solid samples such as fruits, vegetables, and soil) and a certain volume of extract h3 into the pretreatment pool a3 of the microfluidic chip to realize Pesticides were extracted by standing for...

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Abstract

The invention provides a micro-fluidic chip system and a micro-fluidic chip method for pesticide residue detection, wherein the micro-fluidic chip system is composed of a disposable micro-fluidic chip and a portable instrument, the micro-fluidic chip is composed of a fluid inlet / outlet, a micro channel, a reaction pool and a detection pool; a sample solution inflows from the fluid inlet and flows through the reaction pool and the detection pool along the micro channel to finish the enzyme inhibition reaction and chromomeric reaction of a solution phase, and finally the continuous detection of absorbancy is realized in a chromomeric reaction pool or the detection pool by using the portable instrument so as to obtain the pesticide residue analysis result. The micro-fluidic chip system and method for pesticide residue detection provided by the invention are especially suitable for the field, fast, automatic and accurate detection of the pesticide residue in samples such as fruit, vegetable, soil, water quality and the like.

Description

technical field [0001] The invention relates to the field of microfluidic chip analysis, in particular to a microfluidic chip system and method for on-site detection of pesticide residues. Background technique [0002] Pesticides play an important role in agricultural production. In my country's agricultural production, there are a wide variety of pesticides used, including more than 600 types of insecticides, herbicides, fungicides, and rodenticides. Organophosphorus and carbamate pesticides are currently the most widely used pesticides in my country, and most of them are used on fruit and vegetable crops. Therefore, the most prominent pesticide residues in fruits, vegetables, aquatic products, soil and other agricultural products are organophosphorus pesticides and carbamate pesticides. For the monitoring and management of organophosphorus and carbamate pesticides, preventing agricultural products with excessive pesticide residues from being sold on the market has become ...

Claims

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Application Information

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IPC IPC(8): G01N35/00
Inventor 叶嘉明黄昱俊苑宝龙
Owner HANGZHOU TINKER BIOTECHNOLOGY CO LTD
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