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L-lysine fermentation method with short period and high yield

A lysine fermentation technology with a short cycle, applied in the field of L-lysine fermentation, can solve the problems of low acid production, single tank acid production and low conversion rate, and breeding occupation

Active Publication Date: 2014-05-28
ANHUI BBCA BIOCHEMICAL CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the fermentation cycle is also longer. The growth and reproduction of the bacteria in the early stage take up a part of the fermentation cycle. At this time, the acid production is extremely low, and the acid production and conversion rate of a single tank are also low.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] ⑴Inject conventionally cultivated seed tank seeds with 10% glucose 140g / L, ammonium sulfate 50g / L, KH 2 PO 4 ·3H 2 O4.5g / L, MgSO 4 ·7H 2 O0.5g / L, FeSO 4 ·7H 2 O0.015g / L, MnSO 4 ·H 2 O0.015g / L, bean cake hydrolyzate 30g / L, VH150μg, VB11mg, Ph7.2~7.5, sterilized at 121°C for 30min and cooled to 37°C medium, pressure: 0.1MPa; Ph: 6.5-7.0; Air volume: 0.3-0.8vvm; cultivated under DO30-60% conditions. Add sterilized FeSO at 121°C for 30 minutes to sugar feeding 4 ·7H 2 O0.03g / L, MnSO 4 ·H 2 Shake up after O0.03g / L, VH300 μ g / L, VB12mg / L, control the reducing sugar content in the fermented liquid after adding sugar to be 0.5%, and after adding ammonium, the ammonium content in the fermented liquid is 0.3%, until the end of fermentation.

[0026] (2) When the volume of the fermented liquid in (1) is 80% of the volume of the fermenter, start continuous discharge, and maintain the volume of the fermented liquid to be 80% of the volume of the fermenter, and insert the...

Embodiment 2

[0028] ⑴Inject conventionally cultivated seed tank seeds with 10% glucose 140g / L, ammonium sulfate 50g / L, KH 2 PO 4 ·3H 2 O4.5g / L, MgSO 4 ·7H 2 O0.5g / L, FeSO 4 ·7H 2 O0.015g / L, MnSO 4 ·H2 O0.015g / L, bean cake hydrolyzate 30g / L, VH150μg, VB11mg, Ph7.2~7.5, sterilized at 121°C for 30min and cooled to 37°C medium, pressure: 0.1MPa; Ph: 6.5~7.0; Air volume: 0.3-0.8vvm; cultivated under DO30%-60%. Add sterilized FeSO at 121°C for 30 minutes to sugar feeding 4 ·7H 2 O0.03g / L, MnSO 4 ·H 2 Shake well after O0.03g / L, VH300μg, VB12mg, control the reducing sugar content in the fermented liquid after adding sugar to be 0.5%, and after adding ammonium, the ammonium content in the fermented liquid is 0.3%, until the end of fermentation.

[0029] (2) When the volume of the fermented liquid in (1) is 80% of the volume of the fermenter, start continuous discharge, and maintain the volume of the fermented liquid to be 80% of the volume of the fermenter, and insert the discharged ferm...

Embodiment 3

[0032] ⑴Inject conventionally cultivated seed tank seeds with 10% glucose 140g / L, ammonium sulfate 50g / L, KH 2 PO 4 ·3H 2 O4.5g / L, MgSO 4 ·7H 2 O0.5g / L, FeSO 4 ·7H 2 O0.015g / L, MnSO 4 ·H 2 O0.015g / L, bean cake hydrolyzate 30g / L, VH150μg, VB11mg, Ph7.2~7.5, sterilized at 121°C for 30min and cooled to 37°C medium, pressure: 0.1MPa; Ph: 6.5~7.0; Air volume: 0.3-0.8vvm; cultivated under DO30%-60%. Add sterilized FeSO at 121°C for 30 minutes to sugar feeding 4 ·7H 2 O0.03g / L, MnSO 4 ·H 2 Shake well after O0.03g / L, VH300μg, VB12mg, control the reducing sugar content in the fermented liquid after adding sugar to be 0.5%, and after adding ammonium, the ammonium content in the fermented liquid is 0.3%, until the end of fermentation.

[0033] (2) When the volume of the fermented liquid in (1) is 80% of the volume of the fermenter, start continuous discharge, and maintain the volume of the fermented liquid to be 80% of the volume of the fermenter, and insert the discharged fe...

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PUM

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Abstract

The invention relates to an L-lysine fermentation method with a short period and high yield. A flow distribution manner is adopted for feeding fermentation broth discharged from a continuous fermentation process into a new fermentation medium so as to carry out further fermental cultivation under the condition of fed-batch carbon source and nitrogen source. The L-lysine fermented through the fermentation method provided by the invention has the advantages that the period is 32 hours, the acid production rate reaches 18.5 percent, the conversion ratio reaches 72 percent, and the effect is remarkable.

Description

technical field [0001] The invention relates to an L-lysine fermentation method with short period and high yield, belonging to the technical field of biological fermentation. Background technique [0002] It is known that protein is the main component of human cells. After entering the human body, protein in food is first decomposed into amino acids after digestion, and then the human body uses these amino acids to synthesize new human proteins, such as immune antibodies, digestive enzymes, plasma proteins, growth Hormones, etc. are all synthesized human proteins. Among the various amino acids in the synthesis of proteins, L-lysine is the most important one. Without it, other amino acids will be restricted or not utilized. Scientists call it the first essential amino acid for the human body. Scientists have also discovered that L-lysine is the most important and essential component of Somatotation (ss), an important substance that controls human growth, and plays an importa...

Claims

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Application Information

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IPC IPC(8): C12P13/08
Inventor 李荣杰尚海涛杨为华邓远德徐斌戴嘉张雪锋秦晴唐浩张家泉常珠侠纪传侠韩小芬
Owner ANHUI BBCA BIOCHEMICAL CO LTD
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