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Butenolide metabolite and application thereof

A technology of butenolactone and metabolites, applied in application, microorganism, biocide, etc., to achieve obvious inhibitory effect

Inactive Publication Date: 2014-06-18
YUNNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no research report on the use of butenolide compounds as agricultural antibacterial agents

Method used

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  • Butenolide metabolite and application thereof
  • Butenolide metabolite and application thereof

Examples

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Embodiment 1

[0015] The separation and preparation of compound A and compound B:

[0016] 1. Slants Culture of Streptomyces YIM 120811 Strain: Preparation 38 # Solid medium (glucose 4 g, yeast extract 4 g, malt extract 5 g, multivitamin trace 3.7 mg, trace salt 1 mL, agar 13 g, distilled water 1000 mL, pH7.2), after sterilization, make a test tube slope , picking Streptomyces YIM 120811 strains and inserting them into the slant medium, and culturing them to obtain the slant strains;

[0017] 2. Seed Culture of Streptomyces YIM 120811 Strain: Preparation 38 # Liquid medium (glucose 4 g, yeast extract 4 g, malt extract 10 g, multivitamin trace 3.7 mg, trace salt 1 mL, distilled water 1000 mL, pH 7.2), after sterilization, obtain the seed medium, and culture the above The slant strains were transferred to the seed medium, and the seed liquid was obtained after 3 days of shaking culture at 28 °C and 200 r / min;

[0018] 3. Liquid fermentation culture of Streptomyces strain YIM 120811: for...

Embodiment 2

[0021] The antibacterial activity of the butenolide metabolites of the present invention, ie, compounds A and B, was detected by a micro-double dilution method.

[0022] 1. A total of 7 plant pathogenic fungal indicator strains: Alternaria alternateta, Pyricularia oryzae, Fusarium coeruleum, Botrytis cinema, Gibberella saubinetii , apple anthracnose (Colletotrichum gloeosporioides), corn curvularia (Curvularia lunata).

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Abstract

The invention relates to a butenolide metabolite and an application thereof, and belongs to the technical field of microbial pesticides. The production bacterial strain is Streptomyces YIM120811(Streptomycessp.YIM120811) derived from soil and is preserved in typical culture preservation center in China on 19th, September 2013; the preservation number is CCTCCNo:AA2013014. The bacterial strain YIM20811 is subjected to fermenting culture and extracting separation so that two new butenolide compounds A and B are obtained. An antibacterial activity test proves that a butenolide derivative has obvious plant pathogenic fungi activity resistance and has a potential use of preparing a new agricultural antibacterial agent.

Description

technical field [0001] The invention relates to a butenolide metabolite and an application thereof, belonging to the technical field of microbial pesticides. Background technique [0002] Actinomycetes are rich in biodiversity, and their natural products have a wide range of biological activities. They are important resources for discovering new biologically active secondary metabolites. At present, about 75% of the antibiotics used in clinical and agricultural use are produced by actinomycetes, and about 90% of the antibiotics produced by actinomycetes are produced by Streptomyces. Therefore, it is of great research value to discover active secondary metabolites from Streptomyces. Foreign researchers have isolated butenolide compounds from marine-derived Streptomyces (Cho, et al, J. Nat. Prod . 2001, 64 , 664; Mukku et al, J. Nat. Prod . 2000, 63 , 1570). However, there is no research report on the use of butenolide compounds as agricultural antibacterial agents. C...

Claims

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Application Information

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IPC IPC(8): C07D307/58A01N43/08A01P3/00C12P17/04C12R1/465
CPCA01N43/08C07D307/58C12P17/04
Inventor 吴少华姜怡王唐
Owner YUNNAN UNIV
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