Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
A technology of butenolactone and metabolites, applied in application, microorganism, biocide, etc., to achieve obvious inhibitory effect
Inactive Publication Date: 2014-06-18
YUNNAN UNIV
View PDF5 Cites 2 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
However, there is no research report on the use of ...
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Image
Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
Click on the blue label to locate the original text in one second.
Reading with bidirectional positioning of images and text.
Smart Image
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0015] The separation and preparation of compound A and compound B:
[0016] 1. Slants Culture of Streptomyces YIM 120811 Strain: Preparation 38 # Solid medium (glucose 4 g, yeast extract 4 g, malt extract 5 g, multivitamin trace 3.7 mg, trace salt 1 mL, agar 13 g, distilled water 1000 mL, pH7.2), after sterilization, make a test tube slope , picking Streptomyces YIM 120811 strains and inserting them into the slant medium, and culturing them to obtain the slant strains;
[0017] 2. Seed Culture of Streptomyces YIM 120811 Strain: Preparation 38 # Liquid medium (glucose 4 g, yeast extract 4 g, malt extract 10 g, multivitamin trace 3.7 mg, trace salt 1 mL, distilled water 1000 mL, pH 7.2), after sterilization, obtain the seed medium, and culture the above The slant strains were transferred to the seed medium, and the seed liquid was obtained after 3 days of shaking culture at 28 °C and 200 r / min;
[0021] The antibacterial activity of the butenolide metabolites of the present invention, ie, compounds A and B, was detected by a micro-double dilution method.
[0022] 1. A total of 7 plant pathogenic fungal indicator strains: Alternaria alternateta, Pyricularia oryzae, Fusarium coeruleum, Botrytis cinema, Gibberella saubinetii , apple anthracnose (Colletotrichum gloeosporioides), corn curvularia (Curvularia lunata).
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
PUM
Login to view more
Abstract
The invention relates to a butenolide metabolite and an application thereof, and belongs to the technical field of microbial pesticides. The production bacterial strain is Streptomyces YIM120811(Streptomycessp.YIM120811) derived from soil and is preserved in typical culture preservation center in China on 19th, September 2013; the preservation number is CCTCCNo:AA2013014. The bacterial strain YIM20811 is subjected to fermenting culture and extracting separation so that two new butenolide compounds A and B are obtained. An antibacterial activity test proves that a butenolide derivative has obvious plant pathogenic fungi activity resistance and has a potential use of preparing a new agricultural antibacterial agent.
Description
technical field [0001] The invention relates to a butenolide metabolite and an application thereof, belonging to the technical field of microbial pesticides. Background technique [0002] Actinomycetes are rich in biodiversity, and their natural products have a wide range of biological activities. They are important resources for discovering new biologically active secondary metabolites. At present, about 75% of the antibiotics used in clinical and agricultural use are produced by actinomycetes, and about 90% of the antibiotics produced by actinomycetes are produced by Streptomyces. Therefore, it is of great research value to discover active secondary metabolites from Streptomyces. Foreign researchers have isolated butenolide compounds from marine-derived Streptomyces (Cho, et al, J. Nat. Prod . 2001, 64 , 664; Mukku et al, J. Nat. Prod . 2000, 63 , 1570). However, there is no research report on the use of butenolide compounds as agricultural antibacterial agents. C...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to view more 
Login to view more