Method for efficiently extracting phlorizin from plants

A phloridzin and high-efficiency technology, applied in the field of medicine, can solve the problems of high equipment requirements, low extraction efficiency, heat-labile components, etc., and achieve the effects of simple process environmental protection, high extraction yield, and low equipment requirements.

Inactive Publication Date: 2014-06-18
江苏斯威森生物医药工程研究中心有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The constant temperature extraction method takes a long time and the extraction efficiency is low; although the heat reflux extraction method has high extraction efficiency, it still has the disadvantage of long time consumption; the microwave extraction method has the advantages of high extraction efficiency, less solvent use, and can protect heat-lab

Method used

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  • Method for efficiently extracting phlorizin from plants
  • Method for efficiently extracting phlorizin from plants

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Take 1 kg of dried litchi peel powder of 40 mesh, add 5 L of 70% ethanol, conduct ultrasonic treatment at 30 °C for 2 h, then heat and reflux the feed liquid for 3 times for 30 min each time, combine the filtrate, add the total amount of extract to the filtrate 0.2% by weight of antioxidant Vc, distilled under reduced pressure at 60°C until ethanol-free, add 0.5% of the concentrated liquid’s mass of polymerized alumina to the concentrated solution, adjust the pH to 7.5 with 0.3M sodium hydroxide solution, and keep it warm at 60°C 30 min, suction filtration, the filtrate was cooled and extracted 3 times with 1 times the amount of ethyl acetate, the combined extracts were concentrated under reduced pressure to 10:1, then dissolved in 40% methanol, suction filtration, the filtrate was allowed to stand for 24 h, suction Filtrate, dissolve the obtained crystals in water, add activated carbon with 10% of the mass of crystals, stir, filter, let the filtrate stand for 24 hours, ...

Embodiment 2

[0022] Take dried apple branches or leaves and crush them, pass through a 20-mesh sieve, add 10 L of 40% methanol, conduct ultrasonic treatment at 45 °C for 1 h, then heat the feed liquid under reflux and extract 3 times, each time for 1 h, combine the filtrates, and add to the filtrate 0.5% of the total weight of the extract was added to the antioxidant DTT, distilled under reduced pressure at 60°C until there was no ethanol, and 0.7% of the concentrated solution was added to the concentrated solution. Adjust the pH to 7.5 with 0.3M sodium hydroxide solution. Keep warm at 60°C for 30 min, filter with suction, extract the filtrate three times with 1 times the amount of ethyl acetate after cooling, combine the extracts, concentrate under reduced pressure to 10:1, dissolve with 40% methanol, filter with suction, and statically Set aside for 24 h, filter with suction, dissolve the obtained crystals in water, add activated carbon with 10% of the mass of the crystals, stir, filter, ...

Embodiment 3

[0024] The dried pear branches or leaves were crushed, passed through a 20-mesh sieve, 15 L of 40% ethanol was added, ultrasonic treatment was performed at 70 °C for 30 min, and then the material liquid was heated and refluxed for 3 times, each time for 2 h, the filtrate was combined and poured into the filtrate. Add 0.3% of the total weight of the extract to the antioxidant DTT, distill under reduced pressure at 80°C until there is no ethanol, add tartaric acid with a mass of 1% of the concentrate to the concentrated solution, adjust the pH to 7.5 with 0.3M sodium hydroxide solution, and set the temperature at 60°C Keep warm for 30 min, filter with suction, extract the filtrate with 1 times the amount of ethyl acetate three times after cooling, combine the extracts, concentrate under reduced pressure to 10:1, dissolve with 40% methanol, filter with suction, and let the filtrate stand for 24 h , suction filtration, the obtained crystals were dissolved in water, activated carbon...

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Abstract

The invention relates to a method for efficiently extracting phlorizin from plants. The method comprises the steps of crushing dry fruit peels, root barks, roots, leaves and the like of the plants such as apples, pears, litchis and the like which are used as raw materials, and then adding a solvent in a certain proportion; performing pre-treatment by adopting ultrasonic waves, and then performing heat reflux extraction; performing suction filtration, performing decompression concentration on filtrate after adding an antioxidant, adding a flocculant, adjusting the pH value, and then performing suction filtration; cooling the filtrate, concentrating, separating out a crystal, and decolorizing the crystal to obtain a white needle crystal. The method disclosed by the invention is rich in raw material resource, simple in process, high in extraction rate and easy in realization of industrial production.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a method for efficiently extracting phlorizin from plants. Background technique [0002] Phloridin and its glycosides are mainly found in the rhizomes, root barks, leaves and peels of plants such as apples, pears, and lychees, and most of them exist in the form of phloridin. Phlorizin belongs to flavonoid dihydrochalcone, which has the functions of regulating blood pressure and blood sugar, protecting the heart and scavenging free radicals, etc. It has broad application prospects in the fields of medicine and cosmetics. [0003] At present, the methods for extracting phlorizin from plants mainly include constant temperature extraction, heat reflux extraction, microwave extraction and ultrasonic extraction. The constant temperature extraction method takes a long time and the extraction efficiency is low; although the heat reflux extraction method has high extraction ...

Claims

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Application Information

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IPC IPC(8): C07H15/203C07H1/08
Inventor 彭学东张梅赵金召邱洁
Owner 江苏斯威森生物医药工程研究中心有限公司
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