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A kind of compound probiotics and preparation method thereof

A compound microecology and preparation technology, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of insignificant effect of compound microecological preparations, poor strain activity, and increased production costs. Improved average daily gain and immune performance, improved immunity, low cost effects

Active Publication Date: 2016-10-19
六安恒佳生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In order to improve the resistance and store for a long time, it can be microencapsulated during the production process, but the production cost is increased, so the application in the feed industry is limited
[0007] The mixed culture of Lactobacillus casei and Bacillus in the prior art, because Lactobacillus casei is facultative anaerobic bacterium, and the nutrient substance needed for its growth is more complicated than Bacillus, if directly mixed culture, each bacterial strain fails to absorb more. Good nutrition, the activity of the strain is not very good, and the effect of the obtained compound microecological preparation is not significant

Method used

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  • A kind of compound probiotics and preparation method thereof
  • A kind of compound probiotics and preparation method thereof
  • A kind of compound probiotics and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] 1. Culture medium preparation

[0032] YPD medium: including 10g / L yeast powder, 20g / L peptone B and 20g / L D-anhydrous glucose, sterilized at 115°C for 15 minutes;

[0033] MRS medium: including 10.0g / L peptone, 8.0g / L beef powder, 4.0g / L yeast powder, 20.0g / L glucose, 2.0g / L dipotassium hydrogen phosphate, 2.0g / L Diamine hydrogen citrate, 5.0g / L sodium acetate, 0.2g / L magnesium sulfate, 0.04g / L manganese sulfate and 1.0g / L Tween~80, adjust the pH value to 5.7±0.2 at 25°C , sterilized at 118°C for 15 minutes;

[0034] LB medium: including 10.0g / L tryptone, 5.0g / L yeast extract powder and 10.0g / L sodium chloride, adjust the pH value to 7.0±0.1 at 25°C, and sterilize at 121°C for 15 minutes.

[0035] Add 2g / L agar to the above culture medium to prepare a solid culture medium.

[0036] 2. Preparation method

[0037] (1) Cultivation of Lactobacillus casei

[0038] Under sterile conditions, take the freeze-dried strain of Lactobacillus casei, spread it on the MRS solid me...

Embodiment 2

[0048] In this embodiment, the preparation of the culture medium is the same as in Example 1, and the preparation method includes the following steps:

[0049] (1) Cultivation of Lactobacillus casei

[0050] Under sterile conditions, take the freeze-dried strain of Lactobacillus casei, spread it on the MRS solid medium, and culture it statically at 37°C for 20 hours, pick a single colony, and further streak and purify it on the MRS solid medium, 37 After culturing at ℃ for 20 hours, pick a single colony, inoculate it into 100 mL of MRS liquid medium, and culture it statically for 24 hours under anaerobic conditions. At this time, Lactobacillus casei enters the late stage of exponential growth or late stationary phase. Inoculate into MRS culture medium, anaerobic static culture at 37°C for 24 hours to obtain Lactobacillus casei culture fluid;

[0051] (2) Cultivation of Bacillus

[0052] Under sterile conditions, take 0.5g of chicken cecum content, add 4.5mL of sterile saline...

Embodiment 3

[0057] In this example, the bacterial concentration ratio of Bacillus cereus and Bacillus subtilis in step (3) is 1:3; in the mixed culture solution in step (4), Bacillus cereus, Bacillus subtilis and cheese milk The bacterial concentration ratio of bacilli is 1:3:2.5, and the rest of the steps and medium preparation are the same as in Example 2.

[0058] The compound probiotics prepared in Examples 1 to 3 were fed to 1-day-old broiler chicks respectively, and the chicks were divided into four groups, 15 in each group, and the average daily gain was measured within 1 to 42 days, and the broiler chicks were measured in 1-42 days. For the immune organ index at 42 days, in the blank control group, the probiotics were replaced with water, and the positive control was a composite probiotic prepared by lactic acid bacteria, bacillus, yeast zinc, and yeast selenium. The measurement results are shown in Table 1 below:

[0059] Table 1: Analysis of the influence of compound probiotics ...

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Abstract

The invention discloses a composite micro-ecologic preparation and a preparation method thereof. The composite micro-ecologic preparation comprises bacillus cereus, bacillus subtilis and lactobacillus casei, wherein the bacterial concentration ratio of bacillus cereus, the bacillus subtilis and the lactobacillus casei is 1:(1-3):(1-2.5). Compared with the prior art, the composite micro-ecologic preparation has the advantages that as the lactobacillus casei and the bacillus subtilis are mixed and cultured, the synergistic effect of the strains is sufficiently brought into play, protection is provided for aerobic metabolism of lactic acid bacteria, and the utilization rate of equipment is improved.

Description

technical field [0001] The invention relates to a probiotic preparation, in particular to a compound probiotic preparation and a preparation method thereof. Background technique [0002] In recent years, with the widespread use of additives such as antibiotics and chemical drugs in livestock and poultry farming, people have gradually realized the various negative effects brought about by antibiotics and chemical drugs, such as antibiotics leading to animal normal flora imbalance, a large number of Drug-resistant strains are produced; at the same time, toxic and side effects such as drug residues have brought serious harm to animals, humans and the environment, and have reduced the competitiveness of livestock and poultry products in the market. Therefore, many countries have successively banned or restricted the use of antibiotics in feed in the application. In view of the adverse effects of antibiotics, researchers at home and abroad have been committed to developing some ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23K10/18A61K35/747A61P31/04C12R1/125C12R1/085C12R1/245A61K35/742
Inventor 祁克宗谢玲玲涂健汪雪雁薛秀恒付瑞燕张明
Owner 六安恒佳生物科技有限公司
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