Treatment method of scanning electron microscope samples of insect tentacles and appendages

A scanning electron microscope and sample processing technology, applied in the field of scanning electron microscope sample processing, can solve the problems of inability to remove insect antennae and appendage wax layer, incomplete removal of antennae and appendage scales, incomplete cleaning of sample surface, etc., and achieve a balance. The osmotic pressure is unstable, the effect of overcoming the deformation effect, and the low price

Active Publication Date: 2014-06-18
INST OF PLANT PROTECTION HENAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The object of the present invention is to provide a scanning electron microscope sample processing method for insect antennae and appendages, which solves the problem of incomplete removal of scales of antennae and appendages in the pre-processing of insect antennae and appendages, and incomplete cleaning of the sample surface, which cannot be removed. The waxy layer of insect antennae and appendages, it is difficult to penetrate the fixative solution, and the sample becomes brittle and easy to break, etc.

Method used

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  • Treatment method of scanning electron microscope samples of insect tentacles and appendages
  • Treatment method of scanning electron microscope samples of insect tentacles and appendages
  • Treatment method of scanning electron microscope samples of insect tentacles and appendages

Examples

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Effect test

Embodiment 1

[0040] The steps of scanning electron microscope sample preparation for the adult antennae of Spodoptera ervata are as follows:

[0041] (1) Preparation of sample fixing solution, cleaning solution and anesthesia solution

[0042] Prepare phosphate buffer: dissolve potassium dihydrogen phosphate KH in 800 mL of distilled water 2 PO 4 1.4g, disodium hydrogen phosphate dihydrate Na 2 HPO 4 2H 2 O 1.8g, sodium chloride 0.58g and potassium chloride 0.7g, then add 1mol / L hydrochloric acid to adjust the pH to 7.4, and store in a refrigerator at 4°C after high-temperature and high-pressure sterilization.

[0043] The preparation of sample fixing solution: use above-mentioned phosphate buffer solution and the glutaraldehyde solution of commercially available mass concentration 25% and the formaldehyde solution of commercially available mass concentration 38% to be configured into glutaraldehyde-formaldehyde phosphate buffer saline, make glutaraldehyde The mass percentage content...

Embodiment 2

[0055] The steps of scanning electron microscope sample preparation for the adult antennae of Spodoptera ervata are as follows:

[0056] (1) Preparation of sample fixing solution, cleaning solution and anesthesia solution

[0057] Prepare phosphate buffer: dissolve potassium dihydrogen phosphate KH in 500 mL of distilled water 2 PO 4 4.2g, disodium hydrogen phosphate dihydrate Na 2 HPO 4 2H 2 O 5.4g, sodium chloride 1.74g and potassium chloride 2.1g, then add 1 mol / L hydrochloric acid to adjust the pH to 7.2, sterilize at high temperature and high pressure and store in a refrigerator at 4°C.

[0058] The preparation of sample fixing solution: use above-mentioned phosphate buffer solution and the glutaraldehyde solution of commercially available mass concentration 25% and the formaldehyde solution of commercially available mass concentration 38% to be configured into glutaraldehyde-formaldehyde phosphate buffer saline, make glutaraldehyde The mass percentage content is 3%...

Embodiment 3

[0070] The steps of scanning electron microscope sample preparation for the adult antennae of Spodoptera ervata are as follows:

[0071] (1) Preparation of sample fixing solution, cleaning solution and anesthesia solution

[0072] Prepare phosphate buffer: dissolve potassium dihydrogen phosphate KH in 700 mL of distilled water 2 PO 4 7.0g, disodium hydrogen phosphate dihydrate Na 2 HPO 4 2H 2 O 9.0g, sodium chloride 2.9g and potassium chloride 3.5g, then add 1mol / L hydrochloric acid to adjust the pH to 7.0, put it in a refrigerator at 4°C after high-temperature and high-pressure sterilization.

[0073] The preparation of sample fixing solution: use above-mentioned phosphate buffer solution and the glutaraldehyde solution of commercially available mass concentration 25% and the formaldehyde solution of commercially available mass concentration 38% to be configured into glutaraldehyde-formaldehyde phosphate buffer saline, make glutaraldehyde The mass percentage content is ...

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Abstract

The invention relates to a treatment method of scanning electron microscope samples of insect tentacles and appendages, and belongs to the technical field of treatment of experimental samples. The treatment method provided by the invention comprises the steps of anaesthetizing and dissecting insects, fixing a sample through a glutaraldehyde-formaldehyde phosphate buffer, cleaning the sample and performing gradient dehydration with ethyl alcohol on the sample so as to obtain a scanning electron microscope sample before loading. The treatment method provided by the invention has the advantages that the problems that in the earlier stage treatment of the scanning electron microscope samples of the tentacles and appendages, scales of tentacles and appendages are not fully removed, the surface of the sample is not fully cleaned, the sample is hard to soak in a fixing solution thoroughly, and the sample is crisp and is easy to snap are solved, the surface structure of the treated sample is good, the deformation and pollution are not generated, a tissue surface is fully revealed, the sample has a good conductive property, and the real condition of the surface of the sample is effectively reflected.

Description

technical field [0001] The invention relates to a scanning electron microscope sample processing method for insect antennae and appendages, belonging to the technical field of experimental sample processing. Background technique [0002] Antennas and appendages are important sensory organs of insects, on which there are many different types of receptors, and insect receptors are specialized epidermis parts, which are the most basic structural units of insect receptors and insect organisms to perceive the external environment and carry out chemical Communication information receiving device. Through insect receptors, insects can sense volatile compounds in host plants and use this as information for mating, foraging and finding reproductive sites. According to this characteristic, they can be used to monitor and trap insects. The use of insect pheromones to regulate pest behavior is a pollution-free pest control technology. Insects often use chemical odorants to transmit inf...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28G01N1/34
Inventor 田彩红封洪强王瑛胡永敏高丽娜邱峰李国平黄建荣
Owner INST OF PLANT PROTECTION HENAN ACAD OF AGRI SCI
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