A 18o on-line labeled protein quantitative analysis platform and its operation method

A quantitative analysis and operation method technology, applied to the 18O online labeling protein quantitative analysis platform and its operation field, can solve the problems of low labeling efficiency, cumbersome operation and time-consuming, etc., and achieve high labeling time, improved analysis throughput, and pretreatment The effect of step reduction
CN103884807BActive Publication Date: 2015-11-18DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
Publication Date
2015-11-18

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Abstract

The invention relates to a <18>O on-line marked protein quantitative analysis platform, and an operation method thereof. The <18>O on-line marked protein quantitative analysis platform is a system integrating protein on-line enzymolysis, <18>O on-line marking and polypeptide separation and detection. The <18>O on-line marked protein quantitative analysis platform comprises an injection pump, a sampling needle, a column oven, an enzyme reactor, a switch valve, a peptide fragment capture column, a liquid phase chromatography pump, a polypeptide separation column and a mass spectrometry detector. A protein sample is firstly loaded to the enzyme reactor through the injection pump to carry out incubation; <18>O marking can be carried out at the same time with enzymolysis; and the <18>O marked peptide fragment after enzymolysis is enriched on-line and transferred into the polypeptide separation column for further separation, and finally is detected by the mass spectrometry detector. The <18>O on-line marked protein quantitative analysis platform integrates the protein on-line enzymolysis, <18>O on-line marking and polypeptide separation.
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Description

Technical field:

[0001] The present invention relates to a 18 O on-line labeled protein quantitative analysis platform and operation method thereof, 18 O On-line labeled protein quantitative analysis platform is an online enzymatic hydrolysis of proteins, 18 O On-line labeling, peptide separation and detection system. Background technique:

[0002] The main separation technique of protein mixture in the initial stage of proteomics is two-dimensional gel electrophoresis (2DE). The protein mixture is fully separated by first-dimensional isoelectric focusing electrophoresis and second-dimensional size exclusion electrophoresis, and then stained by Coomassie brilliant blue or silver. Staining allows the protein separated on the gel to develop color, and the color intensity represents the corresponding protein content in the sample. The relative content of corresponding proteins in the two samples can be obtained by comparing the color intensity of the proteins at the same pos...

Claims

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