Igg-binding peptide and method for detecting and purifying igg using same

A residue and amino acid technology, applied in the field of human IgG-binding peptides, can solve problems such as utilization limitations

Active Publication Date: 2014-06-25
OTSUKA CHEM CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

For example, non-peptidic affinity ligands based on Protein A mimetic peptides (Non-Patent Documents 3 and 4), or protein A and IgG antibody Fc X-ray crystallographic structures are reported (Non-Patent Document 5) , but in view of their binding ability and specificity, there are limitations in utilization

Method used

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  • Igg-binding peptide and method for detecting and purifying igg using same
  • Igg-binding peptide and method for detecting and purifying igg using same
  • Igg-binding peptide and method for detecting and purifying igg using same

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Embodiment

[0349] Hereinafter, the present invention will be described more specifically with reference to examples, but the scope of the present invention is not limited by these examples.

[0350] In order to isolate human IgG-specific phage from a random peptide library having a circular structure formed by two Cys constructed by the T7 phage display method, the following biopanning method was used.

[0351] That is, 5 × 10 10 pfu T7 phage library (X 3 CX 8 CX 3 、X 3 CX 9 CX 3 x 3 CX 10 CX 3 ) solution was added to a 96-well microplate coated with human IgG-Fc (from human plasma, Athens Research & Technology, Athens, GA, USA) (1 μg / 100 μl / well) and blocked with 0.5% BSA (Nunc, Maxisorp ) wells and allowed to react for 1 hour. After removing the supernatant phage solution, the wells were washed 10 times with PBS containing 0.1% Tween. Escherichia coli BLT5615 (Novagen) culture solution (300 μl) was added to infect it, and cultured together with 3 ml of Escherichia coli cultu...

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Abstract

Provided is a peptide capable of binding to human IgG specifically or selectively. A peptide characterized by comprising an amino acid sequence represented by formula (I): (X1-3)-C-(X2)-H-R-G-(Xaa1)-L-V-W-C-(X1-3) (wherein Xs independently represent an arbitrary amino acid residue other than a cysteine residue; C represents a cysteine residue; H represents a histidine residue; R represents an arginine residue; G represents a glycine residue; Xaa1 represents a glutamic acid residue or an asparagine residue; L represents a leucine residue; V represents a valine residue; and W represents a tryptophan residue) and composed of 13-17 amino acid residues, and also characterized by being capable of binding to human IgG.

Description

technical field [0001] The present invention relates to a human IgG-binding peptide obtained from a random peptide library and a method for detecting and purifying IgG using the peptide. Background technique [0002] At present, antibody drugs are attracting attention as the most reliable molecular targeted drugs, and the field of new drugs is rapidly expanding. Antibody drugs currently under development or in use mostly use antibodies belonging to the immunoglobulin G (hereinafter referred to as "IgG") class. [0003] Conventionally, protein A or protein G derived from Staphylococcus aureus has been used for purification of IgG antibodies (Non-Patent Documents 1 and 2). Since these proteins also bind to the IgG of mice and rabbits, they are mostly used for the purification of IgG at the level of research reagents. However, in recent years, antibody drugs centered on human IgG1 have begun to be used in the field of medicine, and are important in industrial and pharmaceutica...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/09C07K1/16C07K7/08C07K17/00C07K19/00G01N30/88G01N33/53
CPCG01N33/6854C07K1/22C07K17/00C07K17/06C07K7/08G01N2030/8831C07K2319/00
Inventor 伊东祐二
Owner OTSUKA CHEM CO LTD
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