Coaxial electrostatic spinning fibrous scaffold and preparation method thereof

[0044] Example 1

[0045] The embodiment of the present invention provides a coaxial electrospinning fiber support 5 which includes an aliphatic polyester shell layer 51 and a polyvinylpyrrolidone core layer 52.

[0046] The embodiment of the present invention provides a coaxial electrospun fiber support 5 including an aliphatic polyester shell layer 51 and a polyvinylpyrrolidone core layer 52. Because polyvinylpyrrolidone is a safe and non-toxic water-soluble pharmaceutical intermediate and pharmaceutical adjuvant, it can be miscible or compounded with a variety of substances, making the coaxial electrospun fiber stent 5 safe, non-toxic and has excellent biocompatibility ; Because the NH or OH bond of polyvinylpyrrolidone can form an intermolecular association with a variety of drugs and/or biologically active factors, and through this association, the release time and strength of the drugs and/or biologically active factors are controlled, Extend the release and absorption time of the drug and/or biologically active factor in the body, and endow the coaxial electrospun fiber scaffold 5 with the function of loading and slow releasing the drug and/or biologically active factor. It can be seen that the safe and non-toxic coaxial electrospun fiber scaffold 5 provided by the embodiment of the present invention can be widely used in the field of biological tissue engineering.

[0047] Example 2

[0048] The present invention provides a coaxial electrospinning fiber support 5, the coaxial electrospinning fiber support 5 includes: an aliphatic polyester shell layer 51 and a polyvinylpyrrolidone core layer 52 loaded with drugs and/or biologically active factors .

[0049] By adding drugs and/or biologically active factors to the polyvinylpyrrolidone core layer 52, the prepared coaxial electrospun fiber scaffold 5 has a medicinal function and can provide targeted treatment to patients.

[0050] Specifically, the aliphatic polyester is selected from at least one of polycaprolactone, polylactic acid, polylactic acid-glycolic acid copolymer, and poly(lactic acid-hexanol) copolymer, preferably polycaprolactone.

[0051] Based on the requirements for the biodegradability, mechanical properties, and processing properties of the shell, the shell material of the fiber scaffold in the embodiment of the present invention is selected from polycaprolactone, polylactic acid, polylactic acid-glycolic acid copolymer, poly(lactic acid-hexanol) ) At least one of copolymers. Since polycaprolactone has good biocompatibility and biodegradability, good compatibility with other high molecular polymers, good solvent solubility, etc., polycaprolactone is preferred as the shell layer in the embodiments of the present invention. Material, thereby giving the prepared coaxial electrospinning fiber scaffold 5 excellent performance.

[0052] The NH or OH bond based on polyvinylpyrrolidone can form an intermolecular association with a variety of drugs and/or biologically active factors, and through the association, the release time and strength of the drugs and/or biologically active factors are controlled and prolonged The release and absorption time of the drug and/or biologically active factor in the body. It can be seen that the polyvinylpyrrolidone core layer 52 can not only serve as a carrier for drugs and/or biologically active factors, but also can effectively release the loaded drugs and/or biologically active factors, which is widely used in the field of biological tissue engineering.

[0053] Preferably, the drug is bovine serum albumin 6.

[0054] Since bovine serum albumin 6 is a widely used carrier in biochemical experiments, the drug is preferably bovine serum albumin 6 in the embodiments of the present invention. It is understandable that the drug can also be selected from other drugs such as antibiotics, vitamins, proteins and nucleic acids.

[0055] Specifically, the mass ratio of the aliphatic polyester in the aliphatic polyester shell layer, the polyvinylpyrrolidone in the polyvinylpyrrolidone core layer and the drug and/or biologically active factor is 1-1.5:0.35-0.55:0.01-0.015.

[0056] In order to better control the structure of the prepared coaxial electrospinning fiber scaffold 5, make it have a higher specific surface area and porosity, and at the same time make the drug and/or biologically active factor loading and slow release ability at a better In the embodiment of the present invention, the mass ratio of the aliphatic polyester in the aliphatic polyester shell layer, the polyvinylpyrrolidone in the polyvinylpyrrolidone core layer and the drug and/or biologically active factor is controlled within 1-1.5: 0.35-0.55. : 0.01-0.015. Preferably, the mass ratio of the aliphatic polyester in the aliphatic polyester shell layer, the polyvinylpyrrolidone in the polyvinylpyrrolidone core layer and the drug and/or biologically active factor is 1:0.5:0.013.

[0057] Example 3

[0058] As attached figure 1 As shown, the embodiment of the present invention provides a method for preparing a coaxial electrospinning fiber support, including:

[0059] Step 101: preparing an aliphatic polyester shell solution: dissolving the aliphatic polyester in an organic solvent and stirring until completely dissolved to obtain an aliphatic polyester shell solution.

[0060] Step 102: Prepare a polyvinylpyrrolidone core layer solution: dissolve the polyvinylpyrrolidone in an organic solvent, and stir until completely dissolved to obtain a polyvinylpyrrolidone core layer solution.

[0061] Step 103: In a windless environment, inject the aliphatic polyester shell solution and the polyvinylpyrrolidone core solution into the shell solution injector 1 and the core solution injector 2, respectively, and perform coaxial electrospinning to prepare a coaxial Electrospinning fiber support 5.

[0062] In the method for preparing the coaxial electrospinning fiber support 5 provided by the embodiment of the present invention, the nanometer can be prepared by coaxial electrospinning the aliphatic polyester shell solution and the polyvinylpyrrolidone core solution in a windless environment. Class electrospinning fiber scaffold, the nano electrospinning fiber scaffold is safe and non-toxic, and has a high specific surface area and porosity, so that it has a good slow-release function as a carrier of drugs and/or biologically active factors. The method of the invention is simple, easy to control and strong in practicability.