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A kind of method of pre-concentrating and breaking the wall to extract microbial oil

A technology of microorganisms and microbial cells, applied in the dissolution of microorganisms, the preparation method of peptides, the production of fat, etc., can solve the problems of inability to achieve concentration and wall breaking at the same time, large solvent consumption, and high system viscosity, reducing wall breaking. The effect of equipment investment and operating costs

Active Publication Date: 2019-03-15
WILMAR SHANGHAI BIOTECH RES & DEV CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

None of the above concentration methods can achieve concentration and wall breaking at the same time
[0007] From the above analysis, it can be seen that the traditional thermal alkali method for oil extraction by breaking the wall has the disadvantages of large solvent consumption and easy emulsification; the above problems can be solved by breaking the wall after drying, but it brings high energy consumption and low oil extraction rate. The disadvantages such as low level; the method of pre-concentrating and then breaking the wall can overcome the shortcomings of the thermal alkali method and the dry method of breaking the wall to extract oil to a certain extent, but it brings new problems, such as cumbersome process and the introduction of other Substance, high viscosity of the system after breaking the wall, etc.

Method used

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  • A kind of method of pre-concentrating and breaking the wall to extract microbial oil
  • A kind of method of pre-concentrating and breaking the wall to extract microbial oil
  • A kind of method of pre-concentrating and breaking the wall to extract microbial oil

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0072] Embodiment 1: the influence of different pH values ​​on the viscosity of fermented liquid and concentration efficiency

[0073] Weigh 500 g of fermented broth (Schizochytrium sp. self-cultivation, sterilized biomass content 15.5%, dry basis oil content 38.7%) 500g, respectively adjust the pH value of the fermented broth to pH9, pH10, pH11, pH12, and use a rotary evaporator to evaporate and concentrate ( 9000Pa, 80°C, 30min), to investigate the viscosity change and concentration efficiency. Viscosity detection: according to the method in GB / T 22427.7-2008 starch viscosity determination; dry matter concentration detection: according to GB / T 20264-2006, grain, oil moisture twice drying method. The results are shown in Table 1 below.

[0074] The influence of table 1 different pH on the viscosity of fermented liquid and concentration efficiency

[0075]

[0076] It can be seen from Table 1 that the addition of alkali to adjust the pH can reduce the viscosity of the fer...

Embodiment 2

[0077] Embodiment 2: the impact of different alkalis on the foaming phenomenon in the pre-concentration process

[0078] Weigh 500 g of fermentation broth (obtained by Schizochytrium sp. self-cultivation, sterilized biomass content of 15.5%, oil content of 38.7% on a dry basis), adjust the fermentation broth to pH 12 with different lyes, and carry out vacuum pre-concentration (9000Pa, 80°C, 30min), foaming situation.

[0079] The results are shown in Table 2.

[0080] The impact of table 2 different alkalis on the foaming phenomenon in the preconcentration process

[0081]

[0082] *: Na 2 CO 3 Adjust the sample pH to 10.42

Embodiment 3

[0083] Embodiment 3: the oil extraction rate after different alkali preconcentration

[0084] Weigh 1000g of fermentation broth (obtained by Schizochytrium sp. self-cultivation, after sterilization, biomass content 15.5%, dry basis oil content 38.7%), use different lye to adjust the pH of the fermentation broth, and then concentrate at 80°C and 44000Pa for 30min to examine the oil content recovery difference. The results are shown in Table 3.

[0085] Table 3 Oil extraction rate after different alkali preconcentration

[0086]

[0087]

[0088] *First adjust the pH to 12 with KOH, then add 0.2% Ca(OH) 2 powder

[0089] It can be seen from Table 3 that different alkali adjustments can have the effect of concentrating and breaking the wall, and the oil recovery rate of the samples adjusted by KOH and NaOH is relatively high; the pH has a great influence on the oil recovery rate, and KOH adjustment is preferred. pH11.5~pH12.5.

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Abstract

The invention provides a method for preconcentration and wall breaking of microorganism and extraction of microorganism oil, the method is as follows: adjusting the pH value of a fermentation liquid to the range of 10-14, concentrating the fermentation liquid, obtained by the adjusting step, for cell wall breaking of the microorganism, and then extracting oil and other active ingredients after the cell wall breaking. According to the method, preconcentration and wall breaking can be completed in one step, the wall breaking can be completed in the concentration step, the process is simple, the guarant viscosity after the ell wall breaking is low, the follow-up oil extraction operation is facilitated, and the oil and other active ingredients in microorganism cells can be extracted by a simple and easy method.

Description

technical field [0001] The invention belongs to the field of microbial oil extraction, and in particular relates to a method for pre-concentrating and breaking walls to extract microbial oil. Background technique [0002] The microbial cells harvested by cultivation generally exist in the aqueous phase system, with fine particles, high oil content, and tough cell walls. Therefore, the traditional vegetable oil extraction process is not suitable for the preparation of microbial oil. The preparation of general microbial grease comprises 4 steps: 1. Utilize suitable culture medium to culture microorganism in fermentor; 2. Harvest the biomass of microorganism and process (as washing / dehydration or drying); 3. Cell wall breaking, Including physical treatment (cooking, steam explosion), chemical (hot alkali, chelating agent), mechanical (pressing, homogenization, grinding) and other means; 4. Extracting microbial oil from cell debris (such as solvent extraction, direct centrifuga...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/06C07K1/14C11B1/00
Inventor 洪丰王勇姜元荣王野
Owner WILMAR SHANGHAI BIOTECH RES & DEV CENT
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