Streptomyces oxysporum and its application in the control of cruciferous clubroot
A technology of Streptomyces oxysporum, cruciferous family, applied in the directions of application, bacteria, fungicides, etc., can solve problems such as pesticide registration of Streptomyces sp.
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Embodiment 1
[0050] Example 1 Isolation and screening of Streptomyces scabiei WS-24926 and Streptomyces cuspidosporus WS-29246 strains
[0051] Soil samples were taken for separation, purification and preservation by conventional methods. We isolated 30,000 strains of actinomycetes from the soil. Using the method of tracking the activity of the lead compounds, combined with the HPLC-MS natural product compound database, we selected 109 strains of actinomycetes with relatively low toxicity, easy cultivation and industrialization trend. Swelling disease pot experiment and field experiment, the experimental results are shown in Table 1. Finally, it was determined that WS-24926 and WS-29246 were the best, with the smallest disease index, and the corrected preventive effects were 95% and 79%, respectively.
[0052] The implementation steps of the pot experiment: after activating the strains of the tested strains, adopt the secondary shake flask fermentation method, the primary shake flask ferm...
Embodiment 2
[0068] Example 2 Identification of Streptomyces scabiei WS-24926 and Streptomyces cuspidosporus WS-29246
[0069]After morphological observation, physiological and biochemical experiments and molecular biology identification of the screened strains, it was finally determined that WS-24926 was Streptomyces scabbies, and WS-29246 was Streptomyces sp.
[0070] The colony morphology of the Streptomyces scabiei WS-24926 and Streptomyces cuspidosporus WS-29246 strains on the ISP-2 plate of the present invention is shown in the attached figure 1 , figure 2 .
[0071] 16S rDNA sequence analysis: The genomic DNAs of Streptomyces scabiei WS-24926 and Streptomyces cuspidosporus WS-29246 were extracted and amplified by PCR in a 50 μL system. Use primers:
[0072] PrimerA: 5'-AGAGTTTGATCCTGGCTCAG-3';
[0073] PrimerB: 5'-TACGGCTACCTTGTTACGACTT-3'.
[0074] Reaction conditions: pre-denaturation at 94°C for 5 min, 94°C for 1 min, 55.5°C for 1 min, 72°C for 3 min, 35 cycles, and extensi...
Embodiment 3
[0077] Get 4kg xanthan gum and add the water of 112kg to fast shear and mix thoroughly, then add the 800kg described standby fermented bacteria liquid (take each of the three standby fermented bacteria liquids once, the present embodiment can prepare three kinds of Streptomyces Biocontrol suspending agent), 4kg sodium lignin sulfonate, 20kg alkylbenzene sulfonate, 30kg sorbate, 20kg potassium sorbate, 10kg propylene glycol, obtain the mixture material, stir and disperse uniformly, pass through the horizontal sand mill grade one After the secondary grinding, the particle size test shows that more than 90% of the materials have a fineness of less than 5 μm, stop grinding and stir evenly, and package the product as a finished product, that is, the biocontrol suspending agent is obtained.
[0078] Aiming at off-season Chinese cabbage clubroot in high mountains, the root irrigation method was adopted. 100 mL of roots were irrigated at the time of transplanting, and then once every 1...
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