Single root stock bud fast propagation expanding method for miscanthus sacchariflorus

Abstract

The invention discloses a single root stock bud fast propagation expanding method for miscanthus sacchariflorus. The method comprises the steps of (1) field material selecting, (2) bud body selecting, (3) substrate preparing, (4) seedbed preparing, (5) seed sowing, (6) cultivating and (7) transplanting. A miscanthus sacchariflorus community of wetlands is selected in the step of field material selecting. In the step of bud body selecting, rootstocks taken back from the field is washed through run water to remove soil, and the rootstocks are maintained moist. In the step of seed sowing, selected rootstock sections with buds are sown in a furrow horizontally, and the buds are upward. In the step of cultivating, watering is carried out every one or two days to maintain the moist of the substrates, watering is carried out by spraying, the buds start to germinate after one week, and seedlings can reach 15 cm in two weeks. In the step of transplanting, when the seedlings reach 40 cm after four weeks, four to six leaves are grown on each seedling, and new roots are grown on the rootstocks, plants are dug out from the substrates and transplanted into soil. The method is easy to achieve and easy and convenient to operate, the propagation expanding period is short, the germination rate reaches 80%, and the survival rate of transplanting reaches 98%. The cost is low, a great amount of high-quality seedlings of the miscanthus sacchariflorus can be obtained in a short period of time, and the needs of wetland vegetation recovery and the construction of constructed wetlands and industrial raw material bases are met.

Description

technical field [0001] The invention belongs to the field of biotechnology, and more specifically relates to a method for rapid multiplication of a single stem bud of Nandi, which is suitable for the demand for a large number of high-quality Nandi seedlings in wetland restoration, artificial wetland and industrial raw material base construction. Background technique [0002] Nan Di ( Triarrhena lutarioriparia ) are produced in the provinces south of the middle and lower reaches of the Yangtze River in my country, and are mainly distributed in rivers, lakes, and coastal tidal flats, such as Dongting Lake in Hunan and Poyang Lake in Jiangxi. Nandi is a perennial bamboo-like herb with a height of 3-7 meters. Its shape is very similar to that of reed, and it is often mistaken for reed. The main differences between Nandi and reed are: reed has thin stalk wall and large pituitary cavity, while Nandi has thick stalk wall and small pituitary cavity; reed leaves are banded and alter...

AI Technical Summary

Problems solved by technology

[0003] Searched through China Patent Network and related paper websites, and found that there are some reports about the breeding technology of Nandi grass, such as Gao Handong et al. in "Cultivation and Utilization of Digrass" [ China Wild Plant Resources, 2009, 28: (3) 65-67] mentioned underground stem burial propagation, aboveground stem cutting propagation, and aboveground stem layering propagation techniques, but the multiplication coefficient is low, which is not conducive to transplanting; Wang Yuzhen mentioned the seedling pier propagation, rhizome propagation and pressing green method in "Gi's propagation technology" [Special economic animals and plants, 2007, (6): 33], but there is expansion Low coefficient, large damage to the original vegetation, inconvenient transplanting and other problems; Guo Xiayu et al. in "Nandi Tissue Culture and Rapid Propagation Technology" [Ja Plant Physiology, 2011, 47 (10): 987-990] with young panicles as explants, high callus induction, differentiation and rooting rates were obtained, but the cycle was long and the materials were limited by time

Yi Zili et al. established a set of regeneration system in "Rapid Propagation Method of Grass" (application publication number CN 101711504 A) with stems of grass as explants, but hormones and chemicals are needed, and the operation is complicated. Difficult to master