Method for preparation of tobacco flavor by using compound enzymes to catalyze abandoned tobacco waste

A tobacco flavoring and compound enzyme technology, which is applied in the fields of tobacco, application, and tobacco treatment, can solve the problems of complicated processing equipment, environmental pollution, waste of resources, etc., and achieve the effects of natural processing, cost reduction, and high safety

Active Publication Date: 2014-07-16
SHANGHAI TOBACCO GRP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0002] In the production process of cigarettes, especially in each silk-making process section, the processing methods and processing equipment are relatively complicated, and various wastes will inevitably be generated i

Method used

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  • Method for preparation of tobacco flavor by using compound enzymes to catalyze abandoned tobacco waste

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037](1) Reaction: Weigh 10g of waste tobacco powder and a glass reaction bottle with a stopper, add 150mL mass concentration of 1% mannanase, 1% ficinase, 1% glucoamylase, 1% α-amylase, 1% xylanase, 1% pectinase and 1% lipase, 5% pectinase (the mannanase activity ≥ 1000000MNU / g, ficinase activity ≥ 500000MCU / g, glucoamylase activity ≥ 40000μ / g, amylase activity≥40000μ / g, xylanase activity≥1000000μ / g, lipase activity≥1000000LUN / g, pectinase activity≥124000μ / g, all provided by Shanghai Baofeng Biochemical Co., Ltd.) Composite enzyme buffer aqueous solution (pH 5.05), react at 50°C for 24 hours;

[0038] (2) Extraction: After the reaction, filter with non-woven fabric, extract the aqueous solution with 50 mL of ether, extract twice, and extract the filter residue with 50 mL of ether for 15 minutes, and combine the ether phases;

[0039] (3) Concentration: Concentrate the ether phase at 36°C and 850mbar, freeze at -80°C, remove insoluble matter such as wax and fat, and further ...

Embodiment 2

[0050] Use the same volume of water and composite enzyme buffer solution aqueous solution to extract tobacco powder simultaneously, carry out comparative experiment, experimental method is as described in embodiment 1, wherein only composite enzyme buffer solution aqueous solution is replaced by water when using water extraction, other methods are all same. GC / MS was used to compare the changes of some aroma components between the control sample (without enzyme) and the addition of compound enzymes. The results showed that after the compound enzyme post-treatment, the content of solanone was 2.7 times that of the control sample, and damascenone was 2.7 times that of the control sample. 2.2 times, 3-oxo-alpha-ionol is 2.9 times that of the control, benzyl alcohol is 7.1 times that of the control, phenylethyl alcohol is 6.2 times that of the control, 3-hydroxy-beta-damascone is 1.7 times, and giant soybean three Enone 1, 2, 3 and 4 are 1.2 times, 1.4 times, 1.4 and 1.5 times res...

Embodiment 3

[0052] (1) Reaction: Weigh 10g of waste tobacco powder and a glass reaction bottle with a stopper, add 200mL mass concentration of 0.5% mannanase, 2% ficinase, 2% glucoamylase, 2% α-amylase, 0.5% xylanase, 0.5% pectinase and 2% lipase, 3% pectinase (the mannanase activity ≥ 1000000MNU / g, ficinase activity ≥ 500000MCU / g, glucoamylase activity ≥ 40000μ / g, amylase activity≥40000μ / g, xylanase activity≥1000000μ / g, lipase activity≥1000000LUN / g, pectinase activity≥124000μ / g, all provided by Shanghai Baofeng Biochemical Co., Ltd.) Composite enzyme buffer aqueous solution (pH 4.2), react at 45°C for 28h;

[0053] (2) Extraction: After the reaction, filter with non-woven fabric, extract the aqueous solution with 50mL ether, extract twice, extract the filter residue with 40mL ether ultrasonically for 25min, and combine the ether phases;

[0054] (3) Concentration: Concentrate the ether phase at 36°C and 850mbar, freeze at -80°C, remove insoluble matter such as wax and fat, and further c...

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Abstract

The invention relates to the field of tobacco flavor preparation, in particular to a method for preparation of tobacco flavor by using compound enzymes to catalyze abandoned tobacco waste. The method comprises the following steps that (1) a compound enzyme buffer solution aqueous solution is added to the abandoned tobacco waste, and then a reaction is fully carried out; (2) a reaction product obtained through the step (1) is filtered to obtain an aqueous solution, and the obtained aqueous solution is extracted through organic solvent; (3) the organic solvent obtained through the step (2) is concentrated; (4) ethyl alcohol is added to a product obtained through the step (3) to carry out redissolution, the mixture is fully concentrated again to remove residual organic matter, and the obtained product passes through a membrane so that the tobacco flavor can be obtained. According to the method for preparation of the tobacco flavor by using the compound enzymes to catalyze the abandoned tobacco waste, the abandoned tobacco waste generated in the production process is recycled, the cost is reduced, and the economic benefit is increased.

Description

technical field [0001] The invention relates to the field of preparation of tobacco spices, in particular to a method for preparing tobacco spices from waste tobacco powder catalyzed by a composite enzyme. Background technique [0002] In the production process of cigarettes, especially in each silk-making process section, the processing methods and processing equipment are relatively complicated, and various wastes will inevitably be generated in each link, and the waste below a certain mesh number cannot be used for making thin sheets. In the end, it can only be digested by incineration, which not only wastes resources, but also causes environmental pollution. According to statistics, in the United States in 1974, 30 million pounds of tobacco powder waste was produced during the cigarette production process. If these wastes are "resourced", not only can natural chemical raw materials that meet green and hygienic standards be obtained at low cost, but also be able to Prote...

Claims

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Application Information

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IPC IPC(8): A24B15/24
Inventor 杨菁顾文博张元杰王亮孙谢坤刘百战
Owner SHANGHAI TOBACCO GRP CO LTD
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