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Method for preparing tripterygium wilfordii extract by using triterpenes as main components and pharmaceutical application of tripterygium wilfordii extract

A technology of Tripterygium wilfordii and triterpenoids, applied in the treatment of rheumatoid arthritis drugs, the field of extraction optimization of Tripterygium wilfordii extract powder, can solve the problem that the content of triterpenoids is only 15%, and achieve improved bioavailability degree, toxicity reduction, and the effect of solving waste

Active Publication Date: 2014-07-16
ZHEJIANG CHINESE MEDICAL UNIVERSITY +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

201110139090.9 introduced a preparation method of tripterygium wilfordii effective part powder, but its total triterpene content is only 15%

Method used

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  • Method for preparing tripterygium wilfordii extract by using triterpenes as main components and pharmaceutical application of tripterygium wilfordii extract
  • Method for preparing tripterygium wilfordii extract by using triterpenes as main components and pharmaceutical application of tripterygium wilfordii extract
  • Method for preparing tripterygium wilfordii extract by using triterpenes as main components and pharmaceutical application of tripterygium wilfordii extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment 1: prepare the method for the tripterygium officinalis extract powder containing high proportion total triterpenes:

[0024] (1) Take the whole root of Tripterygium wilfordii, crush it, pass through a 40-mesh sieve, and weigh 150g;

[0025] (2) Extract once with 2400mL ethanol ultrasonic (ultrasonic power 250W, speed 750rpm, temperature 20°C), extract for 2 hours, filter the extract, recover the solvent from the filtrate under reduced pressure, obtain a concentrated solution, and dry it in vacuum at 75°C to obtain Tripterygium wilfordii extract Crude powder 10.30g;

[0026] (3) Add 103 mL of ethyl acetate to the crude extract powder to redissolve, filter, and recover the solvent from the filtrate under reduced pressure to obtain 9.28 g of Tripterygium wilfordii extract powder. The total triterpene content is 54.7% determined by UV method, and determined by HPLC Among them, the content of norzera wood aldehyde is 6.2%.

Embodiment 2

[0027] Embodiment 2: prepare the method for the tripterygium twig extract powder containing high proportion of total triterpenes:

[0028] (1) Take the whole root of Tripterygium wilfordii, crush it, pass through a 10-mesh sieve, and weigh 200g;

[0029] (2) Use 1600mL ethyl acetate ultrasonic extraction (ultrasonic power 500W, rotation speed 1250rpm, temperature 50°C) to extract 3 times, 0.5h each time, combine the extracts, filter, and recover the solvent from the filtrate under reduced pressure to obtain a concentrated solution, vacuum at 55°C Dried to obtain 6.46g of the crude product of Tripterygium wilfordii extract powder;

[0030] (3) Add 85 mL of absolute ethanol to the above-mentioned crude extract powder to redissolve, filter, and recover the solvent from the filtrate under reduced pressure to obtain 5.89 g of Tripterygium wilfordii extract powder. The total triterpene content is 72.8% determined by UV method, and determined by HPLC Among them, the content of norze...

Embodiment 3

[0031] Embodiment 3: the method for preparing the tripterygium twig extract powder containing high proportion of total triterpenes:

[0032] (1) Take the whole root of Tripterygium wilfordii, crush it, pass through a 24-mesh sieve, and weigh 200g;

[0033] (2) Ultrasonic extraction with 2000mL acetone (ultrasonic power 400W, rotation speed 1000rpm, temperature 40°C) was used to extract twice, each time for 1.0h, the extracts were combined, filtered, and the filtrate was decompressed to recover the solvent to obtain a concentrated solution, which was vacuum-dried at 70°C. Obtain 5.98g of the crude product of Tripterygium wilfordii extract powder;

[0034] (3) Add 55 mL of acidic ethanol (containing 0.1% hydrochloric acid) to the crude product of the above-mentioned extract powder to redissolve with hydrochloric acid, filter, and recover the solvent from the filtrate under reduced pressure to obtain 5.32 g of tripterygium twig extract powder. The content of triterpene is 68.4%,...

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Abstract

The invention discloses a method for preparing a tripterygium wilfordii extract by using triterpenes as main components and a pharmaceutical application of the triterpenes as main components. The method for preparing the tripterygium wilfordii extract by using triterpenes as main components comprises the following steps: (1) crushing a dried tripterygium wilfordii root medicinal material, screening through a screen of 10-40 meshes, and weighing 150-200g of powder; (2) performing ultrasonic extraction with an extracting solvent, wherein the usage ratio of the extracting solvent to the medicinal materials is (8-16)ml:1g; filtering the extract, reclaiming the filtrate at reduced pressure to extract the solvent to obtain concentrate, and drying to obtain a coarse product of tripterygium wilfordii extract; and (3) adding 5.0-15.0g of the coarse product of tripterygium wilfordii extract into a solvent for re-dissolving, wherein the usage ratio of the solvent to the coarse product of tripterygium wilfordii extract is (10-15)ml:1g, filtering, and reclaiming the solvent at reduced pressure to obtain the tripterygium wilfordii extract. The invention also discloses a medicament prepared from the tripterygium wilfordii extract for treating rheumatoid arthritis. The content of total triterpenes of the prepared tripterygium wilfordii extract is 50-75 percent, so that the utilization of a tripterygium wilfordii medicinal material is improved, and the toxicity of the tripterygium wilfordii medicinal material is reduced.

Description

technical field [0001] The invention belongs to the technical field of extraction of traditional Chinese medicine components, and in particular relates to an extraction optimization method of Tripterygium wilfordii extract powder with total triterpenoids as the main component and its application in medicine for treating rheumatoid arthritis. Background technique [0002] Tripterygium wilfordii Hook.f is a plant of the genus Tripterygium wilfordii Hook.f in the family Euonymus, mainly produced in Zhejiang, Anhui, Jiangxi, Hunan, Fujian and Yunnan. Bitter in the mouth, pungent, cool in nature, very poisonous. Return liver, kidney channel. Efficacy Expelling wind and dampness, dredging collaterals and relieving pain, reducing swelling and pain, detoxifying and killing insects. It is used for damp-heat nodules and cancerous tumors, and it is used clinically to treat leprosy reactions and rheumatoid arthritis. It is often used in Chinese patent medicines such as Tripterygium w...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/37A61P29/00A61P19/02
Inventor 方剑乔马凤森楼芳芳方芳方多凤徐晓勇
Owner ZHEJIANG CHINESE MEDICAL UNIVERSITY
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