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Preparation method and application of a temperature-sensitive composite gel carrier loaded with bioactive factors

The technology of bioactive factor and composite gel is applied in the field of preparation of bioactive factor sustained-release carrier, which can solve problems such as difficulty in denture restoration, influence on patients' chewing function and facial appearance, and achieve the effect of improving bioactivity

Active Publication Date: 2015-08-05
PEKING UNIV SCHOOL OF STOMATOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Alveolar bone atrophy not only makes it difficult to restore the denture, but also affects the patient's masticatory function and facial appearance

Method used

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  • Preparation method and application of a temperature-sensitive composite gel carrier loaded with bioactive factors
  • Preparation method and application of a temperature-sensitive composite gel carrier loaded with bioactive factors
  • Preparation method and application of a temperature-sensitive composite gel carrier loaded with bioactive factors

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Step 1. Dissolve sodium alginate in 100 mL of distilled water at 60°C, while mechanically stirring for 10 minutes, ultrasonicating for 15 minutes, and adjusting the pH of the colloid to 4 with acetic acid to prepare a 2wt% sodium alginate hydrosol;

[0027] Step 2. Take the sodium alginate hydrosol prepared in step 1 separately, add chitosan under stirring conditions, mechanically stir at 60°C for 10 minutes, and ultrasound for 15 minutes to prepare sodium alginate-chitosan hydrosol. The relative weight ratio of chitosan is 0.2;

[0028] Step 3. Prepare a sodium glycerophosphate aqueous solution with a mass concentration of 50%: add the sodium glycerophosphate aqueous solution to the sodium alginate-chitosan hydrosol while stirring. Since the pH of the sol is 4 to 5, control the sodium glycerophosphate / chitosan The mass ratio is 18, forming a uniform sol;

[0029] Step 4. Add 0.1% to 0.3% of adiponectin by weight of the uniform sol to the homogeneous sol prepared in step 3 un...

Embodiment 2

[0035] Step 1. Dissolve sodium alginate in 100 mL of distilled water at 60°C, while mechanically stirring for 10 minutes, ultrasonicating for 15 minutes, and adjusting the pH of the colloid to 4 with acetic acid to prepare a 2wt% sodium alginate hydrosol;

[0036] Step 2. Take the sodium alginate hydrosol prepared in step 1 separately, add chitosan under stirring conditions, mechanically stir at 60°C for 10 minutes, and ultrasound for 15 minutes to prepare sodium alginate-chitosan hydrosol. The relative weight ratio of chitosan is 0.4;

[0037] Step 3. Prepare a sodium glycerophosphate aqueous solution with a mass concentration of 50%: add the sodium glycerophosphate aqueous solution to the sodium alginate-chitosan hydrosol while stirring. Since the pH of the sol is 4 to 5, control the sodium glycerophosphate / chitosan The mass ratio is 22.4, forming a uniform sol;

[0038] Step 4. Add 0.1% to 0.3% of adiponectin by weight of the uniform sol to the homogeneous sol prepared in step 3 ...

Embodiment 3

[0043] Step 1. Dissolve sodium alginate in 100 mL of distilled water at 60°C, while mechanically stirring for 10 minutes, ultrasonicating for 15 minutes, and adjusting the pH of the colloid to 5 with hydrochloric acid to prepare a 3wt% sodium alginate hydrosol;

[0044] Step 2. Take the sodium alginate hydrosol prepared in step 1 separately, add chitosan under stirring conditions, mechanically stir at 60°C for 10 minutes, and ultrasound for 15 minutes to prepare sodium alginate-chitosan hydrosol. The relative weight ratio of chitosan is 0.8;

[0045] Step 3. Prepare a sodium glycerophosphate aqueous solution with a mass concentration of 50%: add the sodium glycerophosphate aqueous solution to the sodium alginate-chitosan hydrosol while stirring. Since the pH of the sol is 4 to 5, control the sodium glycerophosphate / chitosan The mass ratio is 15, forming a uniform sol;

[0046] Step 4. Add 0.1% to 0.3% of adiponectin by weight of the uniform sol to the homogeneous sol prepared in ste...

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Abstract

The invention relates to a preparation method and an application of a bioactive factor loaded temperature-sensitive composite gel carrier. The method comprises the following steps: 1. preparing a sodium alginate hydrosol; 2. preparing a sodium alginate-chitosan hydrosol through the hydrosol prepared by the step 1; 3. preparing a sodium glycerophosphate solution, adding the sodium alginate-chitosan hydrosol under a stirring condition to obtain a uniform sol; 4. adding adiponectin to the uniform sol prepared by the step 3, adding Ca<2+> and uniformly stirring; and 5. adding calcined bone powder to the sol prepared by the step 4 and uniformly mixing to obtain the bioactive factor loaded temperature-sensitive composite gel carrier. The preparation method, for shortcomings of the prior art of repairing missing and defects of an extraction socket wall, can greatly improve bioactivity, biocompatibility, mechanical tolerance and a slow release performance of a normal gel carrier, thus providing a novel repairing material for treatment of repairing missing and defects of the extraction socket wall.

Description

Technical field [0001] The invention belongs to the technical field of preparation of biologically active factor slow-release carriers, and in particular relates to a method for preparing a temperature-sensitive composite gel carrier carrying biologically active factors, and the composite gel carrier is used in orthopedics repair, especially tooth extraction socket alveolar bone Application of wall preservation therapy. Background technique [0002] After the tooth is extracted due to trauma, inflammation and other causes, the remaining alveolar ridge will undergo continuous bone resorption, resulting in a decrease in the height and width of the remaining alveolar bone. Alveolar bone atrophy not only makes it difficult to repair the denture, but also affects the patient's chewing function and facial appearance. Therefore, studying the mechanism and prevention and treatment of residual alveolar bone resorption after tooth loss has important clinical application value for the deve...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/17A61L27/44A61L27/52A61L27/54
Inventor 唐志辉李箐廖运茂
Owner PEKING UNIV SCHOOL OF STOMATOLOGY