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Method for extracting oil in microalgae

An oil and microalgae technology, applied in the direction of oil/fat production, fat production, etc., can solve the problems of cumbersome extraction steps, solvent toxicity environment, high cost, and achieve the effects of short extraction time, simple extraction method and low cost.

Inactive Publication Date: 2014-07-23
BEIJING UNIV OF CHEM TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The present invention designs a method for extracting oil from microalgae, which solves the problems of cumbersome extraction steps, high cost, certain toxicity of solvent and environmental pollution in the method of extracting oil in the prior art

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Weigh 2g of dried algae, dissolve the dried algae in a solvent in a closed reactor, and extract it in a water bath at 70°C for 1 hour to obtain an extract (leaching once in this process for 1 hour, and the following examples also However), the solvent is a mixed solvent of 40ml methanol and ethanol (volume ratio 2:1);

[0039] Centrifuge the extract obtained above at a speed of 5000rpm for 5 minutes to obtain a supernatant, then use the corresponding solvent, that is, a mixture of methanol and ethanol (volume ratio 2:1) to wash the remaining residue twice and centrifuge , combining the liquid obtained by each separation with the supernatant to obtain a supernatant confluence;

[0040] Rotary evaporation of the supernatants pooled together above to remove the mixed solvent of methanol and ethanol in the supernatant pools to obtain crude oil;

[0041] Dissolve the obtained crude oil in n-hexane solvent to obtain a crude oil solution, move the crude oil solution to a sepa...

Embodiment 2

[0044] Weigh 2g of dried algae, dissolve the dried algae in a solvent in a closed reactor, and extract in a water bath at 70°C for 1 hour to obtain an extract. The solvent is 40ml of methanol and isopropanol (volume ratio 2:1) mixed solution;

[0045] Centrifuge the extract obtained above at a speed of 5000rpm for 5 minutes to obtain a supernatant, then use the corresponding solvent, that is, a mixture of methanol and isopropanol (volume ratio 2:1) to wash the remaining residue twice and centrifuge , combining the liquid obtained by each separation with the supernatant to obtain a supernatant confluence;

[0046]Rotary evaporation of the above-mentioned confluent supernatants to remove the mixed solvent of methanol and isopropanol in the supernatants to obtain crude oil;

[0047] Dissolve the obtained crude oil in n-hexane solvent to obtain a crude oil solution, move the crude oil solution to a separatory funnel, and add an equal volume of 1% sodium sulfate solution to wash t...

Embodiment 3

[0050] Weigh 2g of dried algae, dissolve the dried algae in a solvent in a closed reactor, and extract it in a water bath at 70°C for 1 hour to obtain an extract. The solvent is a mixture of 40ml of methanol and ethanol (volume ratio 1:2) solvent;

[0051] The extract obtained above was centrifuged at a speed of 5000rpm for 5 minutes to obtain a supernatant, and then the remaining dregs were washed twice with a corresponding solvent, that is, a mixture of methanol and ethanol (volume ratio 1:2) and centrifuged. The liquid obtained from each separation is combined with the supernatant to obtain a supernatant confluence;

[0052] Rotary evaporation of the supernatants pooled together above to remove the mixed solvent of methanol and ethanol in the supernatant pools to obtain crude oil;

[0053] Dissolve the obtained crude oil in n-hexane solvent to obtain a crude oil solution, move the crude oil solution to a separatory funnel, and add an equal volume of 1% sodium sulfate solut...

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Abstract

The invention relates to a method for extracting oil in microalgae. The method comprises the following steps: 1, dissolving microalgae in a mixed alcohol solvent in a sealed reactor, oscillating, heating and extracting to obtain an extracting liquid; 2, centrifugally separating the extracting liquid obtained in the step 1 to obtain supernatant, repeatedly washing and centrifugally separating the residual dregs by using the same mixed alcohol solvent in the step 1, and merging the liquid separated each time and the supernatant to obtain a supernatant merged liquid; and 3, rotating and evaporating the supernatant merged liquid obtained in the step 2 to remove the mixed alcohol solvent so as to obtain crude oil; and 4, dissolving the crude oil obtained in the step 3 in a normal hexane solvent to obtain a crude oil liquid, transferring the crude oil liquid into a separating funnel, adding deionized water or a neutral salt liquid for washing for several times so as to remove water-soluble substances in the crude oil liquid, collecting the upper organic phase in the washed crude oil liquid, and performing rotary evaporation to remove the normal hexane solvent, thus obtaining total oil.

Description

technical field [0001] The invention belongs to the field of microalgae biotechnology, and in particular relates to a method for extracting oil from microalgae. Background technique [0002] The increasing depletion of fossil fuels and the resulting environmental pollution have made people pay more attention to bio-renewable energy. And food bioenergy will exacerbate the crisis of food shortage, so microalgae as a non-food crop has gained people's favor. Microalgae can grow in various water environments, especially in salt water and sewage waters. Not only does it not compete with food crops for land, but it can also purify the environment. Microalgae grows fast, has high oil content, and also contains Rich in protein and sugar, it can be used as an ideal raw material for animal feed. [0003] Oil extraction from microalgae is a key step in the production of biodiesel. Most of the methods currently used are organic solvent extractions, and most solvent extractions requir...

Claims

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Application Information

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IPC IPC(8): C11B1/10
Inventor 冯嵬黄俊远
Owner BEIJING UNIV OF CHEM TECH
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