Fermentation method capable of enhancing validamycin yield

A fermentation method, the technology of Jinggangmycin, which is applied in the field of fermentation to increase the yield of Jinggangmycin, can solve problems such as limited space for improvement, achieve high production efficiency, shorten the fermentation cycle, and improve the effect of fermentation efficiency and output

Active Publication Date: 2014-07-23
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

And these studies also show that on the basis of the existing high-efficiency fermentation, relying on these technical operations to increase the production of Jinggangmycin, the room for improvement is relatively limited

Method used

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  • Fermentation method capable of enhancing validamycin yield
  • Fermentation method capable of enhancing validamycin yield

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] The effect of the addition time of the fermentation supernatant in the fermentation medium on the production of Jinggangmycin was investigated, and three time periods were selected: 0 h before seed liquid inoculation, 12 h after the start of fermentation, and 24 h after the start of fermentation, and different concentrations of Jinggangmycin were added respectively. The supernatant was fermented for a fermentation test. The implementation steps and results are as follows:

[0032]1. Implementation steps

[0033] (1) Strain activation and plate culture

[0034] The prepared solid spore medium (ingredients: soybean cake powder 2 g, mannitol 2 g, agar press 2 g, tap water 100 mL)

[0035] Sterilize, pour plate and cool before use. Thaw the spore suspension of Streptomyces hygroscopicus 5008 stored at -80°C in a glycerol cryopreservation tube, spread it on a solid medium plate, then invert the plate, and culture it at 37°C for 5-8 days A large number of cyan spores ...

Embodiment 2

[0052] From Example 1, the addition of fermentation supernatant at 12 h in the fermentation medium can promote the increase of Jinggangmycin production. Next, the effects of different supernatant additions on Jinggangmycin production were compared in more detail. The 3 groups added relatively low concentrations of 0.01%, 0.05%, and 0.10%. The implementation steps and results are as follows:

[0053] 1. Implementation steps

[0054] (1) Strain activation and plate culture

[0055] With embodiment 1.

[0056] (2) Seed cultivation

[0057] With embodiment 1.

[0058] (3) Fermentation culture

[0059] Control group: with embodiment 1.

[0060] Fermentation broth addition group: choose a 250 mL spring-loaded Erlenmeyer flask for the fermentation shaker, and fill the bottle with 50 mL of fermentation medium. When transferring, firstly mix the three parallel seed liquids evenly, then pipette 5 mL with a sterilized 5 mL pipette, transfer the seed liquid to a fermentation shaker...

Embodiment 3

[0073] The addition of fermentation supernatant to the fermentation medium at 12 h can promote the increase of Jinggangmycin production. Next, the effects of different supernatant additions on Jinggangmycin production were compared in more detail, and three relatively high addition concentrations were selected. : 0.50%, 1.0%, 1.5%. The implementation steps and results are as follows:

[0074] 1. Implementation steps

[0075] (1) Strain activation and plate culture

[0076] With embodiment 1.

[0077] (2) Seed cultivation

[0078] With embodiment 1.

[0079] (3) Fermentation culture

[0080] Control group: with embodiment 1.

[0081] Fermentation broth addition group: choose a 250 mL spring-loaded Erlenmeyer flask for the fermentation shaker, and fill the bottle with 50 mL of fermentation medium. When transferring, firstly mix the three parallel seed liquids evenly, then pipette 5 mL with a sterilized 5 mL pipette, transfer the seed liquid to a fermentation shaker flask,...

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Abstract

The invention relates to a fermentation method capable of enhancing validamycin yield. The method comprises the following steps: carrying out strain activation and plate culture on a spore suspension to prepare a spore activated solution, inoculating the spore activated solution in a seed culture medium, carrying out primary culture to obtain a seed culture solution, inoculating the seed culture solution in a fermentation culture medium, carrying out fermentation culture for some time, taking the supernate from the fermented culture solution, sterilizing, adding into a fermentation culture medium, and implementing the fermentation and production of validamycin. On the premise of not increasing the energy consumption, the method obtains higher validamycin yield, shortens the fermentation period, lowers the production cost, and has great application value in industrial production.

Description

technical field [0001] The invention relates to a microbial fermentation method in the field of biotechnology, in particular to a fermentation method for increasing Jinggangmycin production. Background technique [0002] Jinggangmycin, also known as effective mycin, is a safe and efficient antifungal antibiotic. At present, Jinggangmycin, as a high-quality pesticide, has achieved remarkable results in the control of rice, corn and wheat sheath blight in major grain-producing areas in my country and Southeast Asia. In addition, as an important raw material for the synthesis of anti-diabetic clinical drugs acarbose (Baitangping) and voglibose (Bexin), the fermentation production of Jinggangmycin has also received extensive attention in the field of medicine. At present, the bacterial strain used in industrialized fermentation is Streptomyces hygroscopicus 5008 variant reported by Shanghai Pesticides ( Streptomyces hygroscopicus var. jinggangensis 5008), although the strai...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/46C12R1/55
Inventor 周文文李玮屠鹏程高慧刘燕郑晓冬
Owner ZHEJIANG UNIV
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