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A rapid detector for residues of β-lactam antibiotics

A technology of antibiotics and detectors, which is applied in the field of rapid detectors for β-lactam antibiotic residues, can solve the problems of low detection limit, time-consuming, and high technical level requirements of analysts, so as to save detection costs and achieve high accuracy. Effect

Active Publication Date: 2016-01-13
SHANDONG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although these methods have good selectivity, high sensitivity, high accuracy, low detection limit, and can detect multiple elements or compounds at the same time, they require expensive instruments and equipment, and the sample pretreatment process is cumbersome, time-consuming, and requires analysts' skills. High level requirements, not suitable for on-site rapid detection

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  • A rapid detector for residues of β-lactam antibiotics
  • A rapid detector for residues of β-lactam antibiotics

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Embodiment 1

[0032] The structural composition of embodiment 1 detector

[0033]Firstly, the structural block diagram of the present invention is described; secondly, the constant potential circuit and the A / D conversion pre-processing circuit included in the signal detection and processing system are described in detail.

[0034] figure 1 It is a structural block diagram of a rapid detector for β-lactam antibiotic residues of the present invention. Such as figure 1 As shown, the present invention consists of a three-electrode system, a signal detection and processing system, a display and printing storage system, and a power supply system. The three-electrode system collects weak current signals through enzyme sensors and immune sensors; the signal detection and processing system performs I / V conversion, amplification, filtering, and A / D conversion on the weak current signals collected by the three electrodes, and finally sends digital signals to The single-chip microcomputer performs ...

Embodiment 2

[0036] Embodiment 2 detection process

[0037] The detection process of a rapid detector for residues of β-lactam antibiotics is as follows: first, put the sensor immobilized β-lactamase into the test bottom solution without antibiotics, and measure the current I when the antibiotics are not included. 1 , that is, the current before inhibition (the current before inhibition can also use the default value set in the system); then take out the working electrode of the enzyme sensor, wash it with distilled water, and put the working electrode immobilized with β-lactamase into the sample solution Incubated, waited for 10 minutes, took it out and washed it with distilled water, put it into the substrate solution again, and measured the inhibited current I 2 ; Finally, the inhibition rate is automatically calculated by the detection program running on the microcontroller, and the calculation formula is as follows:

[0038] I% =( I 1 -I 2 ) / I 1 ×100%.

[0039] If the inhibition...

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Abstract

The invention discloses a rapid detector for beta-lactam antibiotic residues, which belongs to the technical field of animal product safety detection. The β-lactam antibiotic residue rapid detector provided by the present invention is composed of a three-electrode system, a signal detection and processing system, a display and printing storage system and a power supply system. The three-electrode system first uses the enzyme sensor to measure the change of the current before and after contacting the sample, so as to know whether the sample contains β-lactam antibiotics and whether it exceeds the standard. If there are indeed antibiotics exceeding the standard, then use the immunosensor to measure the change of the current before and after contacting the sample, and obtain information such as the β-lactam antibiotic and the concentration of the antibiotic contained in the sample. The detector provided by the invention has the advantages of simple operation, low price, high sensitivity, good repeatability, and can realize online rapid detection, and is suitable for on-site rapid detection of antibiotic residues in livestock products.

Description

technical field [0001] The invention relates to a rapid detector for beta-lactam antibiotic residues, which belongs to the technical field of animal product safety detection. technical background [0002] In recent years, with the rapid development of my country's animal husbandry industry, milk production has increased significantly, and fresh milk and dairy products have become an important part of people's (especially the elderly and children's) daily food. However, the widespread use of antibiotics in modern animal husbandry inevitably results in antibiotic residues in milk. β-lactam antibiotics are a class of broad-spectrum antibiotics derived from microorganisms, and are widely used in the prevention and treatment of livestock and poultry diseases. In order to increase the production of animal food, some are also used as feed additives, which greatly increases the risk of antibiotic residues in food and the environment, causing subsequent human allergic reactions, mic...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/26G01N33/53
Inventor 孙霞郭业民王相友刘君峰赵文苹
Owner SHANDONG UNIV OF TECH