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Preparation method, application, device and detection method of disposable microbial film sensor capable of rapidly detecting water biotoxicity

A technology of microbial film and biological toxicity, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problem of insensitivity of acute toxicity detection of poisons, and achieve good biocompatibility, simple and convenient operation, and rapid field analysis.

Inactive Publication Date: 2016-08-17
TECHNICAL INST OF PHYSICS & CHEMISTRY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are not many reports on using yeast as the subject of acute toxicity detection, mainly because the cell wall of yeast has a strong tolerance to the external adverse environment, which leads to the insensitivity of acute toxicity detection of poisons

Method used

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  • Preparation method, application, device and detection method of disposable microbial film sensor capable of rapidly detecting water biotoxicity
  • Preparation method, application, device and detection method of disposable microbial film sensor capable of rapidly detecting water biotoxicity
  • Preparation method, application, device and detection method of disposable microbial film sensor capable of rapidly detecting water biotoxicity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] A disposable microbial film sensor for rapid detection of water biotoxicity:

[0055] 1. Preparation of disposable microbial film

[0056] Using yeast as the test microorganism, inoculate Saccharomyces cerevisiae (S.cerevisiae) in the liquid medium, the composition of the liquid medium is (mass concentration): yeast extract (1%), peptone (2%), glucose (2%) , grown for 24 hours, centrifuged to obtain wet bacteria, suspended in phosphate buffer solution and stored at 4°C until use.

[0057] In order to improve the analytical sensitivity, the cell wall of the yeast can be pretreated with 30% methanol or ethanol for 12-24 hours to achieve the effect of partially removing the cell wall while maintaining the normal life activities of the microorganism.

[0058] Take 100 μL of the treated yeast liquid and add it to 1 mL of dissolved mixed sol (the mixing volume ratio of polyvinyl alcohol sol (5%) and sodium alginate sol (1%) is 1:0.2), mix well, and drop-coat on On a clean p...

Embodiment 2

[0067] The operation method and steps are the same as those in Example 1 to evaluate the biological toxicity of heavy metal ions. The only change is: the test solution 3,5-dichlorophenol (DCP) solution with different concentrations is configured as the test solution to carry out the water quality acute biological toxicity test , DCP is often used as a reference in international toxicity tests. The results showed that the DCP current decreased in varying degrees after adding toxic substances, which indicated that DCP had obvious toxic effects on yeast. The dose-effect curve is calculated by fitting the hyperbolic function based on the Miman equation, such as Figure 4 As shown, the EC50 value of DCP was 11.45 μg / mL. Compared with the results of the mixed bacteria inspection in Example 5, the sensitivity was significantly improved.

Embodiment 3

[0069] The operation method and steps are the same as those in Example 1 for evaluating the biotoxicity of heavy metal ions, the only change being: preparing organic matter solutions of different concentrations such as the pesticide acephate as the tested solution for water quality acute biotoxicity testing. The results showed that the current decreased to varying degrees after adding the toxic substance acephate, which indicated that the pesticide acephate had obvious toxic effects on yeast. The dose-effect curve is calculated by fitting the hyperbolic function based on the Miman equation, such as Figure 5 Shown, the EC50 value of pesticide acephate is 52.58 μ g / mL. Compared with the results of the E. coli test in Example 4, the sensitivity was remarkably improved.

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Abstract

The invention discloses a preparation method of a one-time microbial film sensor for rapid detection of acute biotoxicity of water, and the preparation method is as follows: mixing evenly a microbial bacterial liquid and mixed sol, then coating a substrate, dropping a curing liquid onto the substrate to obtain a microbial film, taking the microbial film from the substrate to coat the surface of an electrode to obtain the one-time microbial film sensor. By use of the one-time microbial film sensor, the cost of a microbial film sensor in the prior art is reduced greatly, and the operation is more simple. The invention also discloses the application of the one-time microbial film sensor, an electrochemical detection device comprising the one-time microbial film sensor and a detection method. The electrochemical detection device has the characteristics of being quick and sensitive in detection of the biotoxicity of the water, convenient in operation, capable of detecting the biotoxicity of the water in real time and on line, low in cost and the like.

Description

technical field [0001] The invention belongs to the field of microbial sensor technology and environmental monitoring, and in particular relates to a preparation method, application, device and detection method of a disposable microbial film sensor capable of rapidly detecting water body biological toxicity. Background technique [0002] With the rapid development of industrial and agricultural production, more and more toxic substances such as heavy metals and pesticides enter the water body through the process of production and use, causing serious pollution to the water environment. These substances undergo complex physical, chemical and biological transformation processes in the environment, and will form new pollutants. Some pollutants may also enter the food chain and accumulate in organisms, eventually producing various toxic effects on organisms. [0003] Traditional physical and chemical analysis methods can quantitatively analyze the content of a certain type or t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/403G01N27/327G01N27/416
Inventor 只金芳钱俊李久铭
Owner TECHNICAL INST OF PHYSICS & CHEMISTRY - CHINESE ACAD OF SCI
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