Artificial culturing method of lepista sordida mycelium and culturing medium thereof

Abstract

The invention discloses an artificial culturing method of a lepista sordida mycelium and a culturing medium thereof, wherein the culturing medium comprise the following components according to parts by weight: 30-50 parts of pine needle, 5-12 parts of peptone, 6-12 parts of yeast extract powder, 2.5-10 parts of glucose, and 15-20 parts of agar; the artificial culturing method comprises cleaning and boiling the pine needle in water for 20-30 minutes, filtering to extract the juice, metering 1000ml of pine needle juice, then sequentially adding the peptone, the yeast extract powder, the glucose and the agar, boiling and mixing uniformly, subpackaging the prepared culturing medium into a test tube, covering a rubber plug, sterilizing under the temperature of 120-130 DEG C for 20-30 minutes, then taking out the culturing medium, naturally cooling to 20-30 DEG C, making an inclined plane culturing medium, inoculating a purified lepista sordida strain under the aseptic condition, putting the inoculated culturing medium in a constant temperature incubator, culturing for 5-10 days at the temperature of 20-30 DEG C until the mycelium grows fully in the test tube, and then refrigerating the mycelium. According to the artificial culturing method and the culturing medium, the operation is simple, the culturing period is shortened, no pollutants or wastes are produced, and the yield of the mycelium is high.

Description

[0001] Technical field: [0002] The invention relates to a method for artificially cultivating the mycelia of A. variegata and its culture medium, and belongs to the technical field of pharmacognosy. Background technique: [0003] Flower face mushroom belongs to Fungi Kingdom Fungi, Basidiomycota Basidiomycota, Agaricomycetes, Agaricales Agaricales, Tricholomataceae Tricholomataceae, Agaricus Lepista of large fungi. It is fresh, tender and sweet, with strong fragrance and excellent taste. It is known as "one family eats its taste, and ten families smell its fragrance". In Europe, mushrooms are as popular as matsutake and boletus. In France, they are regarded as high-quality ingredients with high food value. [0004] In addition, the flower face mushroom is not only delicious, but also contains many essential vitamins and amino acids and rich protein, such as threonine, alanine, glutamine, serine, glutamic acid, valine, leucine, Asparagine, Aspartic Acid, and Glycine ...

AI Technical Summary

Problems solved by technology

[0006] In recent years, the flower-faced mushroom has gradually been recognized by the world, and the market demand for it has been increasing, which has led to a decrease in wild resources year by year, and it is difficult to meet the market demand. It is imperative to artificially domesticate and cultivate the flower-faced mushroom. The growth rate of the mycelium of the fragrant mushroom is slow, the mycelia are sparse and not strong, which is a technical problem restricting the artificial domestication and cultivation of the fragrant mushroom

At present, most of the artificial cultivation of mushroom mycelium adopts potato dextrose agar medium (PDA); potato comprehensive medium (CPDA); bran glucose agar medium (bran 50-70 g, glucose 20 g, agar 18 -20 g); corn flour comprehensive medium (corn flour 20-30 g, glucose 20 g, potassium dihydrogen phosphate 2 g, magnesium sulfate 0.5 g, agar 18-20 g); compost leach medium [compost ( dry) 100 g, glucose 20 g, agar 18-20 g], etc., these media are used in the process of cultivating the mycelium of the fennel mushroom.