Application of vaccarin for resisting oxidation and high-glucose damage

A technology of flavonoid glycosides and anti-oxidation of Wang Bu Liuxing, which is applied in the directions of medical preparations containing active ingredients, applications, organic active ingredients, etc.

Active Publication Date: 2014-07-30
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] After investigation, there is no relevant literature report (from CNKI, Weipu China Journal Network) that Chinese scholars have found that flavonoid glycosides o

Method used

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  • Application of vaccarin for resisting oxidation and high-glucose damage
  • Application of vaccarin for resisting oxidation and high-glucose damage
  • Application of vaccarin for resisting oxidation and high-glucose damage

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0008] Example 1: Wang Buliu Xing flavonoid glycosides on H 2 O 2 Induces improvement of EA·hy926 cell damage

[0009] Select the cells in the logarithmic growth phase, trypsinize to prepare a cell suspension, adjust the cell concentration, inoculate 8000 cells per well in a 96-well plate, inoculate 160 μL per well, set 4 multiple wells in each group, and randomly group after 24 hours : The normal group and the model group were added with 20μL serum-free medium, and the three treatment groups were added with the corresponding concentration of the same volume of flavonoid glycosides to make the final concentration of the drug 13.76, 6.88, 3.44μmol / L, respectively. After pre-protection at 37℃ for 12h, add 20μL of H to each well in the model group and Wangbuliuxing flavonoid glycoside group 2 O 2 The final concentration is 1000μmol / L. The normal control group is added with 20μL serum-free medium and incubated at 37℃ for 2h. Cell viability is detected by SRB method. See Table 1:

[00...

Example Embodiment

[0019] Example 2: Wangbuliu Xing's flavone glycosides improve the damage of EA·hy926 cells induced by high glucose

[0020] Select the cells in the logarithmic growth phase, trypsinize to prepare a cell suspension, adjust the cell concentration, inoculate 8000 cells per well in a 96-well plate, inoculate 160 μL per well, and randomly group after 24 hours: normal group and model group add 20 μL Serum-free medium, the three treatment groups were added with the corresponding concentration of the same volume of Wangbuluxing flavonoid glycosides to make the final drug concentrations 13.76, 6.88, and 3.44 μmol / L respectively. After pre-protection at 37°C for 12 hours, In the model group and Wang Buliu Xing flavonoid glycoside group, 20μL glucose (final concentration 180mmol / L) was added to each well, and 20μL serum-free medium was added to the normal control group. Each group had 4 multiple wells. After incubating at 37°C for 24 hours, SRB detection Cell viability. See Table 3:

[0021...

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Abstract

The invention provides application of vaccarin for resisting oxidation and high-glucose damage, and belongs to the technical field of traditional Chinese medicament application. The application first discovers that vaccarin has the activity of protecting oxidative stress injury and high-glucose damage of endothelial cells and can be used for treating a series of diseases induced by atherosclerosis, cataract, aged-related macular degeneration and other endogenous or exogenous active oxygen invasions, and preventing and treating diabetes mellitus, hypertension and related diseases caused by high glucose. The vaccarin can be applied to medicinal preparations, nourishment, healthy products, cosmetics and the like.

Description

Technical field: [0001] The invention relates to an application of bulicurin flavonoid glycosides capable of resisting oxidation damage and high glucose damage, and belongs to the technical field of traditional Chinese medicine application. The invention relates to a flavonoid glycoside with anti-oxidation and anti-high sugar damage activity, and clarifies its new application in medicine, food and cosmetics. Background technique: [0002] Wangbuliu is the dry mature seed of Vaccaria segetalis (Neck.) Garcke, a plant of Caryophyllaceae. Buliuxing mainly contains triterpenoid saponins, flavonoid glycosides, cyclic peptides, lipids, fatty acids, monosaccharides and other components [1, Li Fan, Liang Jingyu. Research progress on blanc blanc[J]. Strait Pharmacy, 2007, 19(3):1-5], the flavonoid glycosides of blancalis are light yellow granular crystals, easily soluble in methanol, methanol-water, ethanol, ethanol-water, n-butanol, etc., insoluble in chloroform, ethyl acetate Est...

Claims

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Application Information

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IPC IPC(8): A61K31/7048A23L1/09A61K8/60A61P9/10A61P3/10A61P27/12A61P27/02A61P9/12
Inventor 邱丽颖谢凤珊冯磊杜斌蔡维维李曰金坚
Owner JIANGNAN UNIV
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