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Method and system for fast targeted batch screening of biodiesel microalgae

A biodiesel, batch technology, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of high product price, high processing cost, low algal autotrophic culture density, etc. Harm to the human body, avoid cross-contamination, improve the effect of targeting

Inactive Publication Date: 2014-07-30
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Although microalgae biodiesel is currently technically feasible, compared with fossil diesel, the production of microalgae biodiesel faces two "bottlenecks", which seriously hinder the production of microalgae biodiesel
One is that the production cost of microalgae biodiesel is high, so the product price is also high, which cannot meet the current market demand; the other is that the current algal autotrophic culture density is low, resulting in high cost of downstream processing technology

Method used

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  • Method and system for fast targeted batch screening of biodiesel microalgae
  • Method and system for fast targeted batch screening of biodiesel microalgae
  • Method and system for fast targeted batch screening of biodiesel microalgae

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0070] 1. Experimental method

[0071] 1) Sample processing

[0072] 40 samples of acidic wastewater containing algae (pH=3.0-6.5) were collected from coal-fired power plants, cement plants, and smelters near industrial flue gases in Hunan, Shaanxi, Gansu, and Inner Mongolia, etc., which have been in operation for more than 30 years. Water samples were stored in a refrigerator at 4°C.

[0073] Transfer 100ml of the water sample containing algae collected for enrichment culture, the culture temperature is 28°C, the light intensity is 7500lux, the ventilation rate is 0.33vvm, and CO2 with a volume concentration of 15% is passed through. 2 As a screening pressure, the incubation time was 14 days.

[0074] Cannot tolerate 15% CO through the above enrichment culture 2 Microalgae are eliminated or become vulnerable populations, which can tolerate 15% CO 2 The microalgae are enriched and become the dominant population, and the enriched water sample is obtained.

[0075] Prepare ...

Embodiment 2

[0082] 1. Experimental method

[0083] Select any four of the six strains of microalgae cells screened in Example 1 to cultivate, and detect the content of oil in the microalgae cells, the specific method is as follows:

[0084] 1.1 Algae strain a

[0085] Pick up on solid plates under sterile conditions image 3 The single algae from the source shown in a (the deposit number is CCTCC M2013608, the date of deposit: November 27, 2013; the name of the depository unit: China Center for Type Culture Collection; the abbreviation of the depository unit: CCTCC) fell into the container containing 30ml of sterile culture solution. In a 100ml Erlenmeyer flask, it was cultured statically in a light culture rack at a temperature of 28°C, a light intensity of 7500 lux, a light-to-dark ratio of 12:12, and a culture time of 12 days. It grew to the end of the index to obtain seed liquid.

[0086] Prepare 500mL of BG-11 medium, and insert it into the expanded cell culture medium according to...

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Abstract

The invention discloses a method and system for fast targeted batch screening of dominant target microalgae. The method includes the steps of: collecting samples containing algae from typical industrial sites; conducting restricted enrichment culture on the sample; conducting solid plate dilution dipping cloning of the algae strains subjected to enrichment culture; conducting in situ staining lipid droplets of daughter cells of algae colony; comparing and analyzing the fat content of the screening algae strains to be screened after the staining; and conducting flat transfer of dominant strains. The invention realizes strain fast efficient targeted batch screening of the target microalgae, and the microalgae obtained by using the invention has application potential in production of the raw material for high concentration carbon dioxide fixation coupling of biodiesel.

Description

technical field [0001] The invention relates to the technical field of biomass energy, in particular to a method and a system for rapid batch targeted screening of microalgae strains that can be used to convert industrial flue gas carbon dioxide into triacylglycerides. Background technique [0002] The energy crisis and the increasingly serious greenhouse effect brought about by the burning of fossil fuels directly affect a country's international image and restrict the sustainable development of a country's economy. Therefore, under the situation that the world is facing global warming and promoting sustainable economic and social development, the development of renewable clean energy and CO 2 Emission reduction has become the focus of governments around the world. [0003] Among many renewable energy sources (wind energy, geothermal energy, water energy, solar energy, biomass energy, etc.), biodiesel has a high similarity with petrochemical diesel in terms of energy densi...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12P7/64C12R1/89
CPCY02E50/10
Inventor 缪晓玲巩三强
Owner SHANGHAI JIAO TONG UNIV
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