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Method for preparing environment-friendly high-purity isomaltooligosaccharide

A high-purity isomalto-oligosaccharide technology is applied in the field of preparation of environmentally friendly high-purity isomalto-oligosaccharides, which can solve the problems of waste of isomalto-oligosaccharides and environmental pollution, and can solve the problem of low product purity and simplify the production process. , avoid wasteful effects

Active Publication Date: 2014-07-30
BAOLINGBAO BIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The above literatures mostly focus on the improvement of isomaltooligosaccharide production, but there is little research on how to avoid resource waste and environmental pollution in the production process of isomaltooligosaccharide

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] A preparation method of environment-friendly high-purity isomaltooligosaccharide, characterized in that, the steps are as follows:

[0046] (1) cornstarch is added into deionized water and mixed into starch milk, the concentration of starch milk is adjusted to 16°Bé, pH5.5, and slurry is prepared by pulping;

[0047] (2) According to the ratio of adding 0.35L α-high temperature amylase per ton of starch, add α-high temperature amylase to the slurry whose temperature has dropped to 33°C, and spray once at a temperature of 109°C to obtain liquefaction at a temperature of 95°C liquid;

[0048] (3) Add 0.7L transglucosidase and 0.6L fungal amylase per ton of starch to the liquefied liquid prepared in step (2) Add transglucosidase and fungal enzyme to the liquefied liquid, at a temperature of 62°C 1. Under the condition of pH 6.0, heat preservation for 48h, heat up to 85°C, and heat preservation for 15min to obtain a crude sugar solution with DE≥45%;

[0049] (4) Add gac t...

Embodiment 2

[0056] A preparation method of environment-friendly high-purity isomaltooligosaccharide, characterized in that, the steps are as follows:

[0057] (1) cornstarch is added into deionized water and mixed into starch milk, the concentration of starch milk is adjusted to 19°Bé, pH6.0, and slurry is prepared by pulping;

[0058] (2) Add 0.23L α-high temperature amylase per ton of starch to the slurry whose temperature drops to 35°C, add α-high temperature amylase, and spray once at a temperature of 105°C to obtain liquefaction at a temperature of 97°C liquid;

[0059] (3) Add 0.55L transglucosidase and 0.4L fungal amylase to the liquefied liquid prepared in step (2) at a ratio of 0.55L transglucosidase and 0.4L fungal amylase per ton of starch. 1. Under the condition of pH 5.5, heat preservation for 72 hours, heat up to 70°C, and heat preservation for 15 minutes to obtain a crude sugar solution with DE≥45%;

[0060] (4) Add activated carbon to the rough sugar solution prepared by...

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Abstract

The invention relates to a method for preparing environment-friendly high-purity isomaltooligosaccharide. The method comprises the following steps of (1) adding starch into deionized water, mixing and pulping to obtain pulp; (2) liquefying the pulp with alpha-high temperature amylase to obtain a liquefied solution; (3) saccharifying the liquefied solution to obtain a crude saccharified solution; (4) adsorbing the crude saccharified solution and filtering to obtain a saccharified solution; (5) carrying out ion exchange on the saccharified solution by an ion exchange system to obtain a refined saccharified solution; (6) concentrating the refined saccharified solution to obtain a concentrated solution and then enabling the concentrated solution to pass through a molecular exclusion chromatographic separation system to obtain isomaltooligosaccharide liquid and isomaltooligosaccharide separation mother liquor; (7) concentrating the isomaltooligosaccharide liquid and drying. According to the invention, the isomaltooligosaccharide is separated from the glucose successfully, and impurity molecules such as macromolecular protein are removed at the same time. The existing production process is simplified, the full use of the raw material is made, high-purity glucose solution is obtained, and the economic benefits are obvious.

Description

technical field [0001] The invention relates to an environment-friendly preparation method of high-purity isomaltooligosaccharide, which belongs to the technical field of functional sugar preparation. Background technique [0002] At present, most of the isomaltooligosaccharides use cornstarch as the raw material, and use biological enzyme technology to enzymatically hydrolyze to produce isomaltooligosaccharides, etc., and then use the yeast fermentation method to decompose the glucose into carbon dioxide and ethanol, thereby reducing the glucose content in the product. The highest content of trisaccharides (isomaltose+panose+isomaltotriose) only reaches 45-50%, and the total amount of isomalto-oligosaccharides is between 90-92%. Using this method to produce isomaltooligosaccharide, the glucose produced after enzymatic hydrolysis is an unnecessary component, which must be decomposed and discharged by yeast, resulting in waste of energy and resources; at the same time, the pr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/18C12P19/14
Inventor 刘宗利杨远志王乃强刘峰高义国李克文杨凯琦
Owner BAOLINGBAO BIOLOGY
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