A kind of extraction method of chicken embryo egg yolk phosphoprotein phosphopeptide

Abstract

The invention discloses an extraction method of embryonated egg phosvitin phosphopeptides, which belongs to the technical field of food additives. According to the invention, by collecting embryonated eggs incubated for a certain number of days, and through the approaches of tissue homogenate, centrifugal sedimentation, degreasing and filtering, and the like, phosvitin is extracted; then, under ultrahigh pressure aided conditions, carrying out hydrolysis on the phosvitin; and carrying out ultrafiltration on the obtained product so as to obtain a micromolecule combination, and drying the obtained product so as to obtain a phosphopeptide product. Compared with traditional processes for preparing phosphopeptides from ordinary eggs, the method fully uses the enzymolysis and dephosphorization of phosvitin by embryonated egg endogenous enzymes, thereby eliminating the steps of artificial dephosphorization and the like, so that the process time is greatly shortened, and the cost is saved. The obtained phosphopeptides product has an effect of promoting mineral absorption, therefore, the phosphopeptides product can be developed as a novel functional food, or as a natural food additive for promoting mineral absorption, added into functional foods.

Description

technical field

[0001] The invention relates to a method for extracting phosvitin phosphopeptide from chicken embryo egg yolk, in particular to a method obtained by partially dephosphorizing phosvitin by using endogenous enzyme of chicken embryo egg, and then enzymatically hydrolyzing it with exogenous protease. The invention discloses a method for extracting phosphopeptides for promoting mineral absorption, belonging to the technical field of food additives. Background technique

[0002] In recent years, bioactive peptides have become a hot spot in the research and development of the health care industry. Japan, Europe and the United States have launched various functional foods and food additives containing active peptides. This new type of functional food contributes to the reduction and control of diet-related chronic diseases and has become an industry with great commercial prospects.

[0003] Phosphopeptide is the earliest functional peptide to be researched and devel...

Examples

Embodiment 1

[0022] Such as Figure 4 Shown, a kind of method for extracting the phosphoprotein phosphopeptide of egg yolk of chicken embryo, the steps are as follows:

[0023] (1) Incubation of fertilized eggs: select fertilized eggs that are not harmful to health, and put them in the incubator for incubation; after 12-15 days of incubation, take out the embryonic eggs, put them in a low temperature environment to end their life and save them for processing ;

[0024] (2) Extract phosvitin: Disinfect the surface of chicken embryo eggs, remove the eggshell and take out the chicken embryos, crush the chicken embryos with a tissue homogenizer, dilute with sterile water, adjust the pH to 3~6, ​​and fully stir; Afterwards, the homogenate was centrifuged at 3000g for 20min, the precipitate was collected, and the mixed solution formed after mixing with n-hexane and ethanol in a volume ratio of 3:1 was fully degreased and filtered; the filtered retentate was washed with NaCl solution (1.74 M) D...

Embodiment 2

[0029] The prepared phosphopeptides were tested for promoting calcium absorption, promoting iron absorption and inhibiting linoleic acid oxidation. The specific experimental method is as follows:

[0030] Promote calcium absorption: different concentrations of phosphopeptide solutions (0 mg / L, 200 mg / L, 400 mg / L and 600 mg / L) with 5mM CaCl 2 Mix well with 20mM, 500mL sodium phosphate buffer (pH 8.0). React at 20°C for 30 min, titrate with NaOH to maintain the pH at 7.8, and then determine the soluble calcium content in the supernatant by atomic absorption chromatography. see attached results figure 1 .

[0031] To promote iron absorption: different concentrations of phosphopeptide solutions (0 mg / L, 200 mg / L, 400 mg / L and 600 mg / L) with 5mM FeCl 2 Mix well with 20mM, 500mL sodium phosphate buffer (pH 8.0). React at 20 °C for 30 min, titrate with NaOH to maintain the pH at 7.8, and then determine the soluble iron content in the supernatant by atomic absorption chromatograp...

technical field

[0001] The invention relates to a method for extracting phosvitin phosphopeptide from chicken embryo egg yolk, in particular to a method obtained by partially dephosphorizing phosvitin by using endogenous enzyme of chicken embryo egg, and then enzymatically hydrolyzing it with exogenous protease. The invention discloses a method for extracting phosphopeptides for promoting mineral absorption, belonging to the technical field of food additives. Background technique

[0002] In recent years, bioactive peptides have become a hot spot in the research and development of the health care industry. Japan, Europe and the United States have launched various functional foods and food additives containing active peptides. This new type of functional food contributes to the reduction and control of diet-related chronic diseases and has become an industry with great commercial prospects.

[0003] Phosphopeptide is the earliest functional peptide to be researched and devel...

Examples

Embodiment 1

[0022] Such as Figure 4 Shown, a kind of method for extracting the phosphoprotein phosphopeptide of egg yolk of chicken embryo, the steps are as follows:

[0023] (1) Incubation of fertilized eggs: select fertilized eggs that are not harmful to health, and put them in the incubator for incubation; after 12-15 days of incubation, take out the embryonic eggs, put them in a low temperature environment to end their life and save them for processing ;

[0024] (2) Extract phosvitin: Disinfect the surface of chicken embryo eggs, remove the eggshell and take out the chicken embryos, crush the chicken embryos with a tissue homogenizer, dilute with sterile water, adjust the pH to 3~6, ​​and fully stir; Afterwards, the homogenate was centrifuged at 3000g for 20min, the precipitate was collected, and the mixed solution formed after mixing with n-hexane and ethanol in a volume ratio of 3:1 was fully degreased and filtered; the filtered retentate was washed with NaCl solution (1.74 M) D...

Embodiment 2

[0029] The prepared phosphopeptides were tested for promoting calcium absorption, promoting iron absorption and inhibiting linoleic acid oxidation. The specific experimental method is as follows:

[0030] Promote calcium absorption: different concentrations of phosphopeptide solutions (0 mg / L, 200 mg / L, 400 mg / L and 600 mg / L) with 5mM CaCl 2 Mix well with 20mM, 500mL sodium phosphate buffer (pH 8.0). React at 20°C for 30 min, titrate with NaOH to maintain the pH at 7.8, and then determine the soluble calcium content in the supernatant by atomic absorption chromatography. see attached results figure 1 .

[0031] To promote iron absorption: different concentrations of phosphopeptide solutions (0 mg / L, 200 mg / L, 400 mg / L and 600 mg / L) with 5mM FeCl 2 Mix well with 20mM, 500mL sodium phosphate buffer (pH 8.0). React at 20 °C for 30 min, titrate with NaOH to maintain the pH at 7.8, and then determine the soluble iron content in the supernatant by atomic absorption chromatograp...

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