Culture medium and method for producing multi-component high-activity ganoderma lucidum powder by using same

A technology of Ganoderma lucidum powder and culture medium, which is applied in the field of preparation of multi-component high-activity Ganoderma lucidum and Ganoderma lucidum powder, can solve the problems of inability to meet industrialized fermentation production, unfavorable industrialized production, and low fermentation level, and achieves low cost and environmental protection. Controllable, simple process effects

Active Publication Date: 2014-08-06
DALIAN JIATENG BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the research on Ganoderma lucidum at home and abroad is mainly focused on the fermentation conditions and the separation of active components, and the overall fermentation level is not high. For example, the patent (application number 00111953.2) discloses a liquid fermentation method for simultaneous production of Ganoderma polysaccharides and Ganoderma acid. , the content of ganoderma acid can reach 2.8 mg / 100 mg, and the total amount of intracellular and extracellular polysaccharides can reach as high as 2.34 g / L, but this method is inefficient and cannot meet the needs of industrial fermentation production; and the patent (application number 201210110969.5) has disclosed a A method for increasing the production of total flavonoids in Ganoderma lucidum mycelia by using expansins, that is, by adding expansins to the liquid fermentation medium to increase the production of total flavones, but the method for preparing the expansins liquid used in this patent is complicated, which is not conducive to factories chemical production

Method used

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  • Culture medium and method for producing multi-component high-activity ganoderma lucidum powder by using same
  • Culture medium and method for producing multi-component high-activity ganoderma lucidum powder by using same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] 1) Preparation medium: each liter of medium contains 40g potato juice, 5g rice flour, 6g grape seed powder, 2g ginseng powder, 3g almond powder, 3g wolfberry fruit, 4g hawthorn juice, 5g ginkgo leaf, 0.5g potassium dihydrogen phosphate g, magnesium sulfate 0.5g, glucose 5g, the rest are water, PH is natural;

[0031] 2) Inoculate black Ganoderma (Ganoderma) on the slanted PDA medium for cultivation to obtain slanted strains at a temperature of 25°C for 7 days;

[0032] 3) Inoculate the slanted strain into the PDA liquid seed medium, carry out expansion culture, and cultivate for 5 days at 25°C to obtain liquid seeds;

[0033] 4) Inoculate the liquid seeds into the first-class seed tank at 0.5% of the inoculation amount and cultivate for 48 hours at 27°C to obtain the first-class seeds;

[0034] 5) Inoculate the first-grade seed liquid into the medium described in step 1) according to the inoculum amount of 10%, under the conditions of temperature 27°C, pressure 0.05MPa...

Embodiment 2

[0036] Example 2: The same conditions as Example 1, the species of Ganoderma Lucidum is Ganoderma Lucidum.

Embodiment 3

[0037] Example 3: The same conditions as Example 1, the species of Ganoderma lucidum is Ganoderma neo-japonicum.

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Abstract

The invention discloses culture medium for producing multi-component high-activity ganoderma lucidum by submerged fermentation. Per liter of culture medium contains 1-5g of rice meal, 1-40g of potato juice, 1-6g of amur grape seed powder, 1-2g of ginseng powder, 1-3g of almond meal, 1-3g of barbary wolfberry fruit powder, 1-4g of hawthorn juice, 1-5g of ginkgo leaf, 0.1-0.5g of monopotassium phosphate, 0.1-0.5g of magnesium sulfate, 1-5g of glucose, and the balance of water. The PH value is natural. The multi-component high-activity ganoderma lucidum powder is prepared from the ganoderma lucidum with natural active pharmaceutical ingredients by submerged liquid fermentation by using the culture medium disclosed by the invention. The obtained ganoderma lucidum powder also contains flavonoids compounds and saponin active substances besides active components of the ganoderma lucidum, is pure biological ganoderma lucidum powder which does not contain any hazardous substance, and the culture medium is wide in source, available, and low in cost.

Description

technical field [0001] The invention belongs to the technical field of biological submerged fermentation, and in particular relates to a preparation method of multi-component high-activity Ganoderma lucidum and Ganoderma lucidum fungus powder. Background technique [0002] Ganoderma lucidum: cures all diseases, its functions are fulfilled, it has magical effects, so it is named Ganoderma lucidum, also known as "immortal medicine", commonly known as "Ganoderma lucidum grass", polyporaceae, annual fungi, Ganoderma lucidum as a medicine has been officially included in the National Pharmacopoeia, and it is also known as "Ganoderma lucidum". It is also a new resource food approved by the state. It has no toxic side effects and can be used as medicine and food. Scientific research has shown that Ganoderma lucidum is very rich in pharmacological components, and its active ingredients can be divided into ten categories, including Ganoderma lucidum polysaccharides, Ganoderma lucidum ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A61K36/074C12R1/645
Inventor 王晓坤王玉顺王仁岐
Owner DALIAN JIATENG BIOLOGICAL TECH
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