A kind of clam-derived Bacillus apiary protein and its application and application method in fresh-keeping
A bacillus and honeycomb technology, which is applied in the field of bacillus alveoli from razor clams, can solve the problems of less application of biotechnology, single variety, slow development, etc. Effect
Active Publication Date: 2016-04-27
DALIAN OCEAN UNIV +1
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Problems solved by technology
First, the high price of enzyme preparations limits its popularization and application. Second, only a few natural microbial preservatives are allowed to be used, such as lactococcus and natamycin. Other biotechnology applications are rarely used, and the overall development is slow. single
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[0014] A kind of razor clam source Bacillus alvei protein of the present invention is prepared according to the following steps:
[0015] a. Inoculate the production strain Bacillus alvei110 with 40 g / L glucose and 20 g / L peptone, pH 5 liquid medium, and fill each bottle with 150 mL or 200 mL or 250 mL liquid medium, The temperature is 30°C or 35°C, the rotation speed is 150rpm, 200rpm, or 250rpm, and the shaker is cultivated for 4 days, 5 days, or 6 days. The Bacillus hives was preserved on December 31, 2012 in the General Microbiology Center (CGMCC) of the China Committee for the Collection of Microbial Cultures (CGMCC), located at No. 3, Courtyard No. 1, Beichen West Road, Chaoyang District, Beijing, with a preservation number of CGMCC No: 7079.
[0016] b. Detect the antibacterial activity of the fermentation broth by the filter paper method. Escherichia coli (EC), Proteus vulgaris (PV), Staphylococcusaureus (SA), Enterobacteraerogenes (EA) and Bacillus subtilis (BS) all ...
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A razor clam-derived Bacillus alvei protein and the application and application method of the protein in fresh-keeping of aquatic products. The protein is prepared according to the following steps: the bacterial strain is cultured with razor clam symbiotic Bacillus alvei, and the liquid medium formula is: glucose 40g / L and peptone 20g / L, pH adjusted to 5; 150-250mL liquid culture medium per bottle, temperature 30-35℃, rotation speed 150-250rpm, shaking table for 4-6 days; centrifuge at 4000r / min to remove bacteria The fermentation liquid of the body was concentrated under reduced pressure at 50°C to one-half of the original volume, ammonium sulfate was added, and the saturated final concentration was 90%, and it was left to stand overnight at 4°C, filtered to dissolve the precipitate with PBS buffer, and then the molecular weight cut-off The 3600 Dalton dialysis bag was dialyzed for 4 days, and the content of the dialysis bag was freeze-dried to obtain the protein of Bacillus alveoli derived from razor clam. The preparation process of the invention is simple and the cost is low.
Description
technical field [0001] The invention relates to the field of food preservation, in particular to a razor clam derived Bacillus alvei protein and its application and application method. Background technique [0002] Aquatic products have the characteristics of low fat and high protein, and are an indispensable and important component in a reasonable dietary structure. They have become an important source of animal protein intake for people. In addition, fish, shrimp, crab and other aquatic products are delicious in meat and unique in flavor. They have the reputation of "seafood" and are favored by consumers. The freshness of aquatic products is the most important quality index and the main factor determining its price. In addition, aquatic products are prone to spoilage. Therefore, the research on fresh-keeping technology of aquatic products should be strengthened, the shelf life and shelf life of aquatic products should be extended, and fresh aquatic products It can be tran...
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IPC IPC(8): C12P21/00A23L3/3463A23B4/20C12R1/07
Inventor 张付云苍桂璐杨阳王维才刘晔
Owner DALIAN OCEAN UNIV