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A kind of detection method of authentic pinellia

A detection method and technology of Pinellia, applied in measurement devices, instruments, scientific instruments, etc., can solve the problems of the safety and effectiveness of Pinellia medicinal materials, and it is difficult to distinguish between mixed and counterfeit Pinellia species, and achieve good application prospects, The effect of high accuracy and simple detection method

Active Publication Date: 2015-09-30
CHENGDU UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, in recent years, the market of pinellia medicinal materials has been relatively good, and there have been many counterfeit and confused products in its circulation, which has brought a great impact on the quality, safety and effectiveness of the medicinal materials of Pinellia.
Through research on the market of Chinese herbal medicines, it is found that the counterfeit product of Pinellia is mainly Shui Pinellia, and the main confused products include Arachnium chinensis, Araceae, and Pinellia shanzhu. However, the current Chinese Pharmacopoeia method is difficult to distinguish pinellia and its counterfeit products.

Method used

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  • A kind of detection method of authentic pinellia
  • A kind of detection method of authentic pinellia
  • A kind of detection method of authentic pinellia

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] Embodiment 1 Detection method parameter optimization

[0067] 1. Screening of sample preparation methods

[0068] 1. Experimental method

[0069] Sample preparation method 1: Take 2g of pinellia medicinal material (through a 40-mesh sieve), add 20ml of petroleum ether, ultrasonically extract for 30min, discard the petroleum ether, add 20ml of 95% ethanol, ultrasonically extract for 3 times, combine the extracts and evaporate to dryness, set Make up to 2ml.

[0070] Sample preparation method 2: Take 2g of pinellia medicinal material (passed through a 40-mesh sieve), add 20ml of water for ultrasonic extraction for 30min, take the supernatant, evaporate to dryness, and dilute to 2ml with water.

[0071] Sample preparation method 3: Take 2 g of pinellia herb (through a 40-mesh sieve), add 20 ml of methanol for ultrasonic extraction for 30 min, take the supernatant, evaporate to dryness, and dilute to 2 ml with methanol.

[0072] Sample preparation method 4: Take 2g of pine...

Embodiment 2

[0107] Embodiment 2 Methodological investigation of the method of the present invention

[0108] Adopt the preferred method of embodiment 2, carry out methodological investigation:

[0109] 1. Precision test

[0110] Take the same pinellia sample powder, pass the powder through a No. 4 sieve, prepare samples according to the above method, inject 5 times of samples, 10 μl each time, measure according to the above gradient conditions, investigate the consistency of the retention time of each chromatographic peak, the retention time of each main chromatographic peak RSD Figure 20 .

[0111] 2. Reproducibility test

[0112] Take 5 parts of the same pinellia medicinal material powder, pass the powder through No. 4 sieve, measure according to the above sample preparation method and chromatographic conditions, record the chromatogram, investigate the consistency of the retention time of each chromatographic peak, the RSD of the retention time of each main chromatographic peak Figur...

Embodiment 3

[0115] Embodiment 3 The method of the present invention detects the authentic pinellia

[0116] 1. Experimental method

[0117] Adopt the preferred method of embodiment 2 to prepare need testing solution, accurately draw 10 μ l, inject liquid chromatograph respectively, measure by the chromatographic condition after screening, record the HPLC figure of 45min, adopt " Chinese medicine chromatographic fingerprint spectrum similarity evaluation of State Pharmacopoeia Committee " System (2004A)" for data analysis and processing, matching the spectra of Pinellia, Shui Pinxia, ​​Aconite, and Nanxing samples respectively to generate a comparison spectrum, and then automatically matching the chromatographic peaks measured by the samples with the reference spectrum to generate Pinellia, Shui Pinxia, ​​Baifuzi, and Huzhang Nanxing fingerprints share patterns.

[0118] The standard substances adenosine and guanosine were used for calibration, and the results showed that in the common pa...

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Abstract

The invention discloses a detection method for qualified pinellia tubers. According to the method, determination is performed through HPLC (high performance liquid chromatography) fingerprints and the method comprises the operation steps as follows: (1) taking a to-be-detected sample, crushing the sample, performing extraction with water or methanol serving as a solvent, and preparing a test solution; (2) injecting the test solution into a high performance liquid chromatograph to be determined. Chromatographic conditions are as follows: the detection wavelength is in a range of 254-265 nm; a chromatographic column takes octadecylsilane as filler; and a mobile phase is eluted by a methanol-water system. The detection method for the qualified pinellia tubers is high in precision, good and accurate in reproducibility, convenient to operate, low in cost and broad in market application prospect.

Description

technical field [0001] The invention relates to a detection method for authentic pinellia, belonging to the field of traditional Chinese medicines. Background technique [0002] Pinellia comes from the dried tubers of Pinellia ternata (Thunb.) breit., a plant of the family Araceae. Pinellia resources are widely distributed in my country, except Jilin, Heilongjiang, Inner Mongolia, Xinjiang, Qinghai, and Tibet, and other provinces and regions are distributed. [0003] In recent years, the market demand of pinellia has continued to increase, while wild resources have decreased year by year, coupled with the slow development of artificial cultivation of pinellia, resulting in a sharp decline in the reserves and output of pinellia resources. In addition, in recent years, the market of pinellia medicinal materials has been good, and there have been many counterfeit and confused products in its circulation, which has brought a great impact on the quality, safety and effectiveness...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06
Inventor 李敏卢道会杨小艳敬勇
Owner CHENGDU UNIV OF TRADITIONAL CHINESE MEDICINE