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Lentinan, extraction and purification method thereof and application of lentinan as tobacco humectant

A technology of lentinan and a purification method, which is applied in the directions of tobacco, application, and treatment of tobacco, to achieve the effects of reducing impurities, obvious moisturizing effect, and broad application prospect.

Inactive Publication Date: 2014-08-13
CHINA TOBACCO HENAN IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are no relevant reports on the application of lentinan as a tobacco humectant in cigarettes at home and abroad

Method used

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  • Lentinan, extraction and purification method thereof and application of lentinan as tobacco humectant
  • Lentinan, extraction and purification method thereof and application of lentinan as tobacco humectant

Examples

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Embodiment 1

[0018] A method for extracting and purifying lentinan, comprising the following steps: extracting 300 g of dried shiitake mushrooms with 1800 ml of ether Soxhlet for 2 hours to remove oil, and evaporating the residue to dryness. The residue after evaporating to dryness was extracted three times with 750ml boiling water, combined the boiling water extracts obtained three times, concentrated, centrifuged, and the supernatant was taken, and the supernatant was added with absolute ethanol 3 times the volume of the clear liquid for alcohol precipitation, and stood overnight (12h), centrifuge again to collect the precipitate. The precipitate was washed three times with ethanol and acetone in turn, then dissolved in distilled water, then papain with a weight of 1‰ of the precipitate was added and placed in a water bath at 50°C for 2 hours, and the protein was removed by the Sevage method. Add 8g of activated carbon and place it in a 70°C water bath to heat for 3 hours, remove the act...

Embodiment 2

[0021] A method for extracting and purifying lentinan, comprising the following steps: extracting 300 g of dried shiitake mushrooms with 2000 ml of ether Soxhlet for 4 hours to remove grease, and evaporating the residue to dryness. The residue after evaporating to dryness was extracted with 750ml of boiling water for 3 times, and the boiling water extracts obtained three times were combined, concentrated, centrifuged, and the supernatant was taken, and anhydrous ethanol with 3 times the volume of the clear liquid was added for alcohol precipitation, and stood for 12 hours , and centrifuge again to collect the precipitate. The precipitate was washed three times with ethanol and acetone in turn, then dissolved in distilled water, then papain with a weight of 2‰ of the precipitate was added, placed in a water bath at 50°C for 4 hours, and the protein was removed by the Sevage method. Add 8g of activated carbon and place it in a 70°C water bath to heat for 4 hours, remove the acti...

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Abstract

The invention relates to a lentinan as well as an extraction and purification method thereof. The extraction and purification method concretely comprises the steps of carrying out Soxhlet extraction on the smashed dry mushrooms by using diethyl ether, then, volatizing residues, and next, extracting the residues by using boiling water; centrifuging and centrifuging a boiling water extracting solution, carrying out ethanol precipitation on a supernatant, and standing; centrifuging again, collecting a sediment, and dissolving by using distilled water after washing; then, adding 0.1-0.2% of papain into a water bath with the temperature of 50+ / -5 DEG C for 2-4h; removing proteins by using a Sevage method, and carrying out vacuum concentration after activated carbon decoloration; dialyzing flowing water after cooling; after dialysis is ended, carrying out ethanol precipitation again and standing, and drying the sediment at the temperature of 60-70 DEG C to obtain an earthy yellow crude polysaccharide. A test proves that the lentinan provided by the invention has favorable physical and sensory humectant effects, can be used for reducing the irritation and miscellaneous odor of cigarettes and improving the mellow, full and comfort levels of smoke of the cigarettes, and can be used as a performance-excellent tobacco humectant to be applied to the cigarettes.

Description

technical field [0001] The invention belongs to the technical field of moisturizers, and in particular relates to a lentinan, an extraction and purification method and its application as a moisturizer for tobacco. Background technique [0002] At present, glycerin, propylene glycol, sorbitol and other polyhydroxy substances are mainly used as humectants in the cigarette industry in my country. The main purpose of using such humectants is to maintain the moisture content of shredded tobacco during processing and improve the processing resistance of shredded tobacco. The above-mentioned humectant has unsatisfactory effects on maintaining the moisture content of finished cigarettes and improving smoking comfort. [0003] Polysaccharides are composed of a large number of monosaccharides, and a large number of hydroxyl groups form hydrogen bonds with water molecules, which can make them have strong water holding capacity. At present, there are no relevant reports on the applicati...

Claims

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Application Information

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IPC IPC(8): C08B37/00A24B15/40
Inventor 陈芝飞马宇平芦昶彤孙志涛郝辉宋金勇
Owner CHINA TOBACCO HENAN IND
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