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Cellulomonas bacterial strain, green alga hydrolysis method, health food preparation method and prepared health food

A cellulomonas and green algae technology, applied in microorganism-based methods, food preparation, biochemical equipment and methods, etc., can solve the problems that algae are difficult for human nutrition supplementation and medical application, and the human small intestine cannot digest and absorb. , to achieve the effect of simple and convenient hydrolysis and nutrient retention

Inactive Publication Date: 2014-08-13
NUGEN BIOSIENCE TAIWAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, all green algae, such as Chlorella and Scenedesmus, cannot be digested and absorbed by the human small intestine due to their hard cell walls, so that algae are difficult to be used for human nutritional supplements and medical applications

Method used

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  • Cellulomonas bacterial strain, green alga hydrolysis method, health food preparation method and prepared health food
  • Cellulomonas bacterial strain, green alga hydrolysis method, health food preparation method and prepared health food
  • Cellulomonas bacterial strain, green alga hydrolysis method, health food preparation method and prepared health food

Examples

Experimental program
Comparison scheme
Effect test

preparation example

[0040] Materials and Methods

[0041] 1. Cellulase activity analysis

[0042] Put 100 microliters (μL) of extracellular cellulase into 20 millimolar concentration (mM) phosphate buffer (pH 7.0) containing 0.1% 900 μL carboxymethyl cellulose (CMC) and mix evenly. After reacting at 50°C for 30 minutes, 1 milliliter (mL) of dinitrosalicylic acid (DNS) was added, and heated in boiling water for 5 minutes to stop the reaction. After cooling to room temperature, the absorbance value was Measured at 540 nanometers (nm), the definition of one unit of extracellular cellulase activity: how much enzyme can hydrolyze carboxymethyl cellulose and release 1 microgram (μg) of glucose at 50°C and 30 minutes ) (Miller, Anal Chem 31:426-8, 1959).

[0043] The activity of neutral protease is measured according to the method described in (Anson, J Gen Physiol 22:77-89, 1938): 12 mL of crude enzyme (crude enzyme) is added to 48 mL of azotyrosine at a concentration of 2% protein solution (azocase...

Embodiment 1

[0074] The chlorophyll change that embodiment 1 hydrolyzes green algae cell (thermotolerant chlorella) gained

[0075] In this example, 20% green algae suspension and 150 U / mL cellulase extracellular cellulase obtained by the method of "decomposing the cell wall of microalgae" were measured at the absorbance value of 675nm to 430nm. According to (Raychaudhuri B, APPL Phys B91:545-50, 2008), all chlorophyll can form two obvious absorption bands under blue light (402nm to 441nm) and red light (640nm to 680nm), such as figure 1 As shown, when the absorbance value is 750nm, the chlorophyll increases from 0.46 to 0.86 after being hydrolyzed by crude enzyme 150U / mL for 60 minutes.

Embodiment 2

[0076] Embodiment 2 Cellulase hydrolyzes the influence of green algae cell (thermotolerant chlorella)

[0077] This example is the 10% suspension of microalgae obtained by "decomposing the cell wall of microalgae" described in the preparation example, and the microalgae was mixed with different concentrations (0U / mL, 150U / mL, 200U / mL, 250U / mL, 300U / mL and 350U / mL) of cellulase, after hydrolysis at 50°C for 180 minutes, the methods described above in "Soluble Protein", "Chlorophyll Release" and "Peptide Content" were carried out to evaluate the effect of cellulose hydrolysis on green algae cells.

[0078] Such as Figure 2A As shown, after 30 minutes of hydrolysis, the protein content increased from 2.03 mg / mL to a range between 15.39 mg / mL and 20.87 mg / mL, and after 180 minutes of hydrolysis, the protein content increased to 25.37 mg / mL to 27.25 mg / mL Between mL; such as Figure 2B As shown, when hydrolyzing for 30 minutes, the chlorophyll content increased from 1.87 mg / mL t...

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Abstract

The invention provides a cellulomonas bacterial strain, a green alga hydrolysis method, a health food preparation method and prepared health food, and particularly provides the cellulomonas bacterial strain (Cellulomonas sp.YJ5); the cellulomonas bacterial strain has the ability of green algae hydrolysis; the invention also provides the green alga hydrolysis method using the cellulomonas bacterial strain, and the green alga hydrolysis method comprises cultivating of the cellulomonas bacterial strain and mixing of the cellulomonas bacterial strain with a green algae suspension liquid for the green algae hydrolysis; the invention also provides the health food preparation method using the green algae hydrolyzed by the cellulomonas bacterial strain, wherein the cellulomonas bacterial strain can promote the release of soluble proteins, chlorophyll, peptides and reducing sugar in the green algae, and can enhance the ferrous chelating ability, Trolox equivalent antioxidant capacity and reducing sugar ability.

Description

technical field [0001] A cellulomonas strain, especially a cellulomonas strain capable of hydrolyzing green algae, and a method and application of using the strain to hydrolyze green algae. Background technique [0002] Cellulase plays an important role in effective biological hydrolysis (effective biological hydrolysis), and through endo-β-1,4-glucanase (endo-β-1,4-glucanase) (EC3.2.1.4, EG), cellobiohydrolase (cellobiohydrolase) (EC3.2.1.91, CBH) and β-D-glucosidase (β-D-glucosidase) (EC3.2.1.21) synergistic action (synergistic action) to make cellulose broken down into simple sugars. In the prior art, cellulase is used in many industrial applications such as animal feed (animal feed), textile (textile), waste water (waste water), brewing (brewing) and wine-making (wine-making), these cellulase is It consists of independently folding, discrete units called domains or modules, each with structure and function, which constitute the unit of cellulase. The action mode of th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/06A23L1/29C12R1/01A23L33/00
Inventor 殷俪容江善宗
Owner NUGEN BIOSIENCE TAIWAN
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