Serratia marcescens M9 and application thereof to remove heavy metal ions
A technology of Serratia marcescens and heavy metal ions, which is applied in the direction of bacteria, microorganism-based methods, and the restoration of polluted soil, etc., can solve the problem of low heavy metal tolerance, large impact, strain screening, and characteristic growth conditions of strains. Less research, etc.
Active Publication Date: 2014-08-13
润桐(苏州)技术服务有限公司
View PDF4 Cites 4 Cited by
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
In the past ten years, the research on heavy metal-resistant microorganisms in my country has mainly focused on the properties of microorganisms and the influence of heavy metals on bacterial cells, while there are few studies on strain screening, strain characteristics, and growth conditions.
At present, there are few isolated heavy metal-resistant populations, the growth and reproduction of bacteria are greatly affected by the habitat environment, and the ability to remove heavy metals is not strong, and it cannot meet the complex pollution status of the external actual environment. ability to remove
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View moreImage
Smart Image Click on the blue labels to locate them in the text.
Smart ImageViewing Examples
Examples
Experimental program
Comparison scheme
Effect test
Embodiment Construction
[0111] Remove heavy metal ions Mn 2+ strain screening
[0112] 1 Experimental materials
[0113] 1.1 Experimental strains
[0114] Sample source
[0115] Soil or water samples from different locations were collected from Weifang, Rizhao, Nanjing and other areas. The soil samples were collected from 1-4cm topsoil in multiple places in the sampling area and mixed separately. There are 27 samples in total.
[0116] 1.2 Experimental Instruments
[0117] Vertical pressure steam sterilizer (Hirayama HVE-50, Japan); air bath shaker (IKA KS 4000ic, Germany); analytical balance (Mettler Toledo PL 602-S, Switzerland); constant temperature incubator (MMM Incucell 111, Germany); gun (Eppendorf, Germany); desktop high-speed centrifuge (Eppendorf 5417R, Germany); inductively coupled plasma mass spectrometry (ICP-MS, Agilent 7700X, USA), etc.
[0118] 1.3 Experimental reagents
[0119] Manganese chloride [MnCl 2 ×4H 2 O] Chemical reagents were of analytical grade. Calculate the mas...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More PUM
Login to View More Abstract
The invention discloses serratia marcescens M9 and application thereof to remove heavy metal ions. Serratia marcescens M9 has removal effect on a heavy metal ion Mn<2+>. A method for screening out serratia marcescens with the removal effect on the heavy metal ion Mn<2+> from soil and water comprises the following steps: a, preparation of a metal selective medium; b, enrichment domestication of a heavy-metal-tolerant bacterial strain; c, screening and secondary screening on the heavy-metal-tolerant bacterial strain; and d, detection on the heavy metal removal capability of the bacterium. By employing the medium containing metal ions to perform enrichment domestication on the heavy-metal-tolerant bacterial strain and performing detection experiments on heavy metal removal capability of the purified bacterium, a reasonable effective method for screening the heavy-metal removing bacterial strain is established, and the suitable needed bacterial strain is obtained. Serratia marcescens M9 is applied to treatment of heavy-metal pollution, and has the advantages of being free of secondary pollution, high in treatment efficiency, wide in application scope, low in cost and the like.
Description
technical field [0001] The invention relates to a strain of microorganism and its application in removing heavy metal ions, in particular to a strain of Serratia marcescens and its application in removing heavy metal pollution, belonging to the technical field of detection and application of microorganisms. Background technique [0002] Heavy metals are widely distributed in the atmosphere, biosphere, lithosphere, and hydrosphere. After being discharged into the environment, they are permanent and have obvious cumulative effects. They can pollute water bodies and soils and eventually accumulate in organisms through food chain cycles, resulting in serious Harm the ecological environment and human health. [0003] The technology of microbial treatment of heavy metal pollution is an emerging field that is constantly being explored and developed in the world. The use of biosorbents prepared by microbial systems to treat heavy metal pollution is currently the most promising metho...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More Application Information
Patent Timeline
Login to View More IPC IPC(8): C12N1/20C02F3/34B09C1/10C12R1/43C02F101/20
Inventor 赵晗孙军丁葵英刘永强
Owner 润桐(苏州)技术服务有限公司