A kind of Ganzhou bradyrhizobium and its application

A technology of slow rhizobium and inoculum, which is applied in the field of microorganisms to achieve broad application prospects and strong resistance to stress.

Inactive Publication Date: 2017-01-18
RES INST OF TROPICAL FORESTRY CHINESE ACAD OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the bottleneck restricting the development of my country's microbial fertilizer industry is the selection of excellent strains

Method used

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  • A kind of Ganzhou bradyrhizobium and its application
  • A kind of Ganzhou bradyrhizobium and its application
  • A kind of Ganzhou bradyrhizobium and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1: Isolation and Identification of Bradyrhizobium Ganzhou RITF806, RITF807, RITF211

[0026] 1. Isolation of Rhizobium

[0027] The strain was isolated from the root nodules of 12-year-old Acacia blackwood in Ganzhou, Jiangxi Province. Rinse the surface of the root nodule with tap water to clean the sediment, soak it in 95% alcohol for 15-30 seconds, put it into 0.1% mercuric chloride solution for surface disinfection for 3-5 minutes, and rinse it with sterile water for 7-8 times. Put the root nodules after surface disinfection between two sterile glass slides, squeeze them hard to break the root nodules, use a sterile inoculation needle to dip the root nodule liquid, draw a line on the YMA plate, and culture in a constant temperature incubator at 28-30°C When colonies appear, typical colonies are picked for purification and identification. Rhizobia RITF806, RITF807 and RITF211 were thus purified.

[0028] 2. Identification of Rhizobia

[0029] Rhizobium RIT...

Embodiment 2

[0065] Embodiment 2, Ganzhou Bradyrhizobium ganzhouense (Bradyrhizobium ganzhouense) RITF806 inoculation effect

[0066] 1. Bacteria culture

[0067] The Bradyrhizobium ganzhou strain RITF806 was activated on the YMA slant and transferred to the YMA liquid medium, and cultured on a shaking table at 28-30°C (150 rpm) to the late logarithmic growth stage.

[0068] 2. Inoculation

[0069] The seedlings are made of Acacia acacia tissue culture seedlings, the substrate is non-sterilized yellow heart soil, a plastic pot with a diameter of 12 cm is selected, double-layer filter paper is placed on the bottom of the pot, and yellow heart soil is added to the surface of the pot 1.5 cm. Acacia tissue culture bottle seedlings with similar growth were taken, the root agar was cleaned with distilled water, and then planted in plastic pots filled with yellow core soil. One seedling was transplanted in each pot, and the seedlings were managed according to conventional seedling raising method...

Embodiment 3

[0079] Example 3. Bradyrhizobium ganzhouense RITF806, RITF211 and RITF807 strains were used to inoculate Acacia black wood, Acacia ganzhouense and Acacia ash.

[0080] 1. Bacteria culture

[0081]Bradyrhizobium ganzhou strains RITF806, RITF211 and RITF807 were activated on YMA slant and transferred to YMA liquid medium, and cultured on a shaking table at 28-30°C (150 rpm) until late logarithmic growth.

[0082] 2. Seed treatment and germination

[0083] First, soak the seeds in concentrated sulfuric acid for half an hour, then pour out the concentrated sulfuric acid, wash with sterile water for 7-8 times, and soak in sterile water until the seeds swell. The treated seeds were placed in a sterile petri dish (inner lined with sterile filter paper and sterile water for moisturizing), and placed in a 30°C incubator to germinate.

[0084] 3. Inoculation and seedling cultivation

[0085] After the seeds germinated, the seeds were planted in test tubes filled with sterile vermicul...

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Abstract

The invention discloses bradyrhizobium ganzhouense sp.nov. and application thereof. Bradyrhizobium ganzhouense sp.nov. RITF806 is preserved in China Center for Type Culture Collection (CCTCC), Wuhan University, Wuhan, China since 26th Feburary, 2014, and the preservation number is CCTCC NO: M2014044. Bradyrhizobium ganzhouense sp.nov. is separated and screened out from acacia melanoxylon rootnodule, is capable of realizing nodulation symbiosis with acacia melanoxylon, acacia aneura, acacia implexa and the like, has high stress resistance, and has wide application prospect for azotification microbe inocula and bio-organic fertilizers.

Description

[0001] Technical field: [0002] The invention belongs to the field of microorganisms, and in particular relates to Bradyrhizobium ganzhou and its application. [0003] Background technique: [0004] The atmosphere contains 78% nitrogen, which cannot be directly used by animals and plants. Only some prokaryotic microorganisms in nature have the ability to directly use nitrogen in the atmosphere and reduce it to ammonia, which is nitrogen fixation. The world's population, resources, and environment have become global issues today. The biological nitrogen fixation known as the "green revolution" has been paid more and more attention, especially in the application of forestry. Because forestry has the characteristics of long production cycle and complex site conditions, the fertilization of forestry is not as convenient and effective as agriculture. Therefore, biological nitrogen fixation has become an important source of nitrogen required by forest trees. [0005] The forestry ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C05F11/08C12R1/01
Inventor 康丽华陆俊锟窦雅静朱亚杰王胜坤隋新华
Owner RES INST OF TROPICAL FORESTRY CHINESE ACAD OF FORESTRY
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