Preparation method for autologous-serum antigen-sensitized DC-CIK cells
A DC-CIK, autologous serum technology, applied in the fields of tumor immunotherapy and cell biology, can solve problems such as unfavorable promotion, high cost, serum pollution, etc., to avoid changes in antigen structure, low equipment requirements, and avoid resistance. Effect
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[0029] The following examples illustrate the present invention, but the present invention is not limited thereto. In the following examples, all experimental methods without specific conditions are carried out in accordance with conventional methods and manufacturer's operating instructions.
[0030] Routinely collect 50-100ml of peripheral blood of patients with advanced lung cancer, 5-15IU / ml of heparin sodium for anticoagulation, rotate at 800g, centrifuge for 10 minutes, collect the upper plasma part (about 1 / 3 of the liquid layer), and add the final concentration of 0.1-1.0 mg / ml leupeptin inhibitor, mix well, then store in a sterile centrifuge tube and freeze for 24-48h in an environment below -20°C (see figure 1 ), after taking it out, put it in a 0-4℃ environment for 10-16h (see figure 2 ); Inactivated at 56°C for 30 minutes, filtered with a 0.22μm microporous membrane to obtain autologous serum tumor antigens.
[0031] Density gradient centrifugation is used to separate P...
PUM
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