Method for preparing xylose and glucose from Zizania aquatica waste

A glucose and waste technology, applied in the field of glucose preparation, achieves the effects of mild reaction conditions, recyclable utilization, and reduced production costs

Active Publication Date: 2014-08-13
TAIZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there are few reports on the utilization of Zizania waste resources, and the present invention proposes a process for extracting sugar resources from Zizania waste

Method used

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  • Method for preparing xylose and glucose from Zizania aquatica waste
  • Method for preparing xylose and glucose from Zizania aquatica waste
  • Method for preparing xylose and glucose from Zizania aquatica waste

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0085] Firstly, the Zizania sheath leaves, leaves and leaf sheaths of Zizania wastes are dried overnight at 50°C, crushed with a pulverizer to particles with a particle size of 10-20mm, and leached with 90% ethanol at a temperature of 90°C for 1 hour. The solid-liquid ratio (g / ml) is 1:10, filter, and extract the obtained solid with formic acid at 70°C for 2 hours, the solid-liquid ratio (g / ml) is 1:10, filter, and separate the solid and liquid. The liquid is distilled under reduced pressure, and when the formic acid is recovered, the solid is obtained, which is the prepared xylose, and the recovery rate of xylose is 95% after detection and analysis; the obtained solid continues to use formic acid, and is leached at a temperature of 90 ° C for 5 hours , the solid-to-liquid ratio (g / ml) is 1:20, and the reaction produces glucose with a purity of 95%.

Embodiment 2

[0087] First dry the leaves, leaves and sheaths of Zizania zizania waste at 50°C overnight, crush them with a pulverizer to particles with a particle size of 10-20mm, and extract them with 80% ethanol at a temperature of 80°C for 2 hours. The solid-liquid ratio (g / ml) is 1:10, filter, and extract the obtained solid with formic acid at 75°C for 2 hours, the solid-liquid ratio (g / ml) is 1:20, filter, and separate the solid and liquid. The liquid is distilled under reduced pressure, and when the formic acid is recovered, the solid is obtained, which is the prepared xylose, and the recovery rate of xylose is 93% after detection and analysis; the obtained solid continues to use formic acid, and is leached for 4 hours at a temperature of 80°C , the solid-liquid ratio=1:10, the reaction produces glucose, and the purity of the glucose reaches 94%.

Embodiment 3

[0089] First, dry the leaves, leaves and leaf sheaths of Zizania zizania waste at 50°C overnight, crush them with a pulverizer to particles with a particle size of 10-20mm, and extract them with 70% ethanol at a temperature of 90°C for 1 hour. The solid-liquid ratio (g / ml) is 1:5, filter, and extract the obtained solid with formic acid at 70°C for 1 hour, the solid-liquid ratio (g / ml) is 1:10, filter, and separate the solid and liquid. The liquid is distilled under reduced pressure, and when the formic acid is recovered, the solid is obtained, which is the prepared xylose, and the recovery rate of xylose is 92% after detection and analysis; the obtained solid continues to use formic acid, and is leached for 4 hours at a temperature of 90°C , the solid-to-liquid ratio (g / ml) is 1:20, and the reaction produces glucose with a purity of 92%.

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Abstract

The invention discloses a method for preparing xylose and glucose from Zizania aquatica waste, which comprises the following steps: 1) pulverizing Zizania aquatica waste, leaching in 70-90% ethanol at 80-90 DEG C for 1-2 hours, and filtering to obtain a solid; 2) leaching the solid in formic acid at 60-75 DEG C for 1-2 hours, filtering, carrying out solid-liquid separation, distilling the obtained liquid under reduced pressure, and recovering the formic acid, thereby obtaining the solid xylose; and 3) continuing leaching the solid obtained in the step 2) in formic acid at 80-90 DEG C for 4-5 hours to obtain the glucose. By using the Zizania aquatica waste as the raw material, the method implements regeneration and reutilization of the Zizania aquatica waste, and has the advantages of mild reaction conditions, lower energy consumption, high recovery rate of xylose and glucose, and safe and nontoxic product.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for preparing xylose for filamentous fungal cell culture and glucose for lactic acid and fumaric acid fermentation. Background technique [0002] With the increasing oil crisis, the raw materials of the world chemical industry are gradually changing from petroleum resources to biomass resources. At present, most of the raw materials of biochemical industry are starch and sugar prepared from grain, which has led to an increasingly serious situation of "competing with humans (animals) for grain and land with grain". As the main raw material of biochemical industry, biomass monosaccharide has become a bottleneck restricting the development of China's biochemical industry. [0003] Lignocellulose includes agricultural production waste and residues (such as crop straw, chaff, bran, bagasse, etc.), forest wood (softwood and hardwood) and forestry processing waste, gra...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C13K13/00C13K1/02C12N1/14C12P7/56C12R1/845
CPCY02P20/59
Inventor 付永前蒋茹尹龙飞朱华跃李鑫
Owner TAIZHOU UNIV
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