Specific primers and probe used for detecting mutation of MP (mycoplasma pneumoniae) 23S rRNA (ribonucleic acid)

Abstract

The invention relates to the technology of primers and probes and aims to provide specific primers and a probe used for detecting mutation of MP (mycoplasma pneumoniae) 23S rRNA (ribonucleic acid). The primers used for detecting mutation of an MP 23S rRNA2617 locus C:A specifically comprise an upstream primer MPF with a sequence shown in SEQ NO.1 and a downstream primer MPR with a sequence shown in SEQ NO.2. The detection sensitivity of the primers and probe provided by the invention can reach 1000 copies / mL, thereby indicating good sensitivity of the primers and the probe. The primers and the probe do not have detection signals for samples without chlamydia trachomatis, thereby indicating strong specificity of the primers and the probe and avoiding false negative and false positive results. Utilized to carry out fluorescent PCR (polymerase chain reaction) treatment, the primers and probe provided by the invention are simple and convenient to operate, are quick in detection and have high detection rates. Compared with the traditional isolated culture detection technology, the primers and the probe improve the detection efficiency and reduce the omission rate. The primers and the probe can efficiently and reliably screen various clinical samples, monitor clinical conditions and provide a guide in rational drug use.

Description

technical field [0001] The invention relates to primer and probe technology, in particular to a specific primer and probe for detecting 23S rRNA2617 site C:A mutation of Mycoplasma pneumoniae. Background technique [0002] Mycoplasma pneumoniae (Mycoplasma pneumoniae, MP) is a kind of prokaryotic cell-type organism without cell wall between bacteria and viruses. Can survive outside the body without relying on living cells and can replicate itself. It is mainly transmitted through the respiratory tract, and children and young people are susceptible. It is generally sporadic or a small epidemic. It can occur all year round, and it often breaks out in autumn. The epidemic trend is that it will spread globally every 3-7 years, and it will occur in densely populated places such as schools. The rate is high, and it is easy to infect each other among family members. From the popular reports in recent years, it can be seen that the incidence of MP pneumonia is on the rise, account...

AI Technical Summary

Problems solved by technology

[0003] At present, the clinical detection of drug resistance of Mycoplasma pneumoniae mainly relies on the isolation of Mycoplasma pneumoniae and drug sensitivity test. Because the separation of Mycoplasma pneumoniae is difficult, the sensitivity is low, and it is time-consuming and labor-intensive, which is not conducive to timely guidance of medication

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