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Method for purifying oligomeric proanthocyanidin, method for controlling degree of polymerization, hyaluronidase inhibitor, and collagenase inhibitor

A technology of oligomeric proanthocyanidin and purification method, which is applied in the fields of purification of oligomeric proanthocyanidin, adjustment of polymerization degree, hyaluronidase inhibitor and collagenase inhibitor, to achieve high yield, excellent wrinkles, excellent Effects of Hyaluronidase Inhibition

Active Publication Date: 2014-08-20
SUNTORY HLDG LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since a mixture of proanthocyanidins that has nothing to do with the degree of polymerization is also extracted during the extraction, it is difficult to separate and purify OPC with target physiological activity from the obtained extract with high purity.

Method used

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  • Method for purifying oligomeric proanthocyanidin, method for controlling degree of polymerization, hyaluronidase inhibitor, and collagenase inhibitor
  • Method for purifying oligomeric proanthocyanidin, method for controlling degree of polymerization, hyaluronidase inhibitor, and collagenase inhibitor
  • Method for purifying oligomeric proanthocyanidin, method for controlling degree of polymerization, hyaluronidase inhibitor, and collagenase inhibitor

Examples

Experimental program
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Embodiment

[0121] The following examples are given to illustrate the present invention in more detail, but these do not limit the present invention in any way.

[0122] In the examples, alcohol concentration (%) is volume % (v / v %) unless otherwise specified.

[0123] In the following preparation examples, the grape press obtained in the white wine production process, that is, the squeezed pulp (peel and seed of grapes) obtained by squeezing juice from grape fruit was used as the grape press for white wine. As grape presses for red wine, squeezed juices obtained by fermenting grape presses obtained in the red wine production process, that is, crushed liquid of crushed grape fruit (juice including pulp, peel, and seeds), and pressing the fermented liquid Meal (peel and seeds).

preparation example 1

[0125] Preparation of proanthocyanidins from white wine grape press (Chardonnay)

[0126] (1) Extraction by hot water

[0127] Add 10 times the amount of water as the extraction solvent to the pressed product of Chardonnay (grape variety) obtained in the white wine manufacturing process (Chardonnay peel and seeds, 250 g), and extract by heating at 80°C for 1 hour . The obtained hot water extract was roughly filtered through a nylon mesh (manufactured by RIKEN KK, trade name: NRS-200) to obtain Extract 1. Furthermore, the filtered residue was also extracted and filtered again under the same conditions, and the obtained extract 2 was combined with the above-mentioned extract 1, and concentrated under reduced pressure to 400 mL. The obtained concentrated solution was filtered with celite, and the filtrate was introduced into a column filled with an acrylic resin Amberlite XAD (registered trademark)-7HP (ORGANO Co., Ltd., 100 mL), and then passed through with water (200 mL). Re...

preparation example 2

[0131] Preparation of proanthocyanidins from red wine grape press (Cabernet Sauvignon)

[0132] (1) Extraction by hot water

[0133] Add 10 times the amount of water as the extraction solvent to the pressed product of Cabernet Sauvignon (grape variety) obtained in the red wine production process (Cabernet Sauvignon peel and seeds, 30 g), and extract by heating at 80°C for 1 hour . The obtained hot water extract was roughly filtered through a nylon mesh (manufactured by RIKEN KK, trade name: NRS-200) to obtain an extract. The obtained extract was concentrated to 10 mL under reduced pressure, mixed with water to 45 mL, and left to stand in a refrigerator for 3 days. Then the extract was used for centrifugation (3000rpm, 5 minutes), and its supernatant was introduced into a chromatographic column filled with acrylic resin Amberlite XAD (registered trademark)-7HP (ORGANO company, 20mL). Water (40mL) was passed through to remove excess sugars and organic acids (elution of sugars...

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Abstract

A method for purifying an oligomeric proanthocyanidin, comprising an extraction step of extracting a raw material containing multiple types of oligomeric proanthocyanidins having different degrees of polymerization with an extraction solvent selected from water and an aqueous alcohol solution, wherein the alcohol concentration in the extraction solvent is preset in accordance with the degree of polymerization of a desired oligomeric proanthocyanidin in the extraction step and the oligomeric proanthocyanidin having a desired degree of polymerization is extracted using the extraction solvent.

Description

technical field [0001] The present invention relates to a purification method for obtaining oligomeric proanthocyanidins having a good whitening effect from raw materials containing oligomeric proanthocyanidins with different degrees of polymerization, a method for adjusting the degree of polymerization of oligomeric proanthocyanidins, and Hyaluronidase inhibitors, collagenase inhibitors, etc., in which anthocyanins are active ingredients. Background technique [0002] External skin preparations and cosmetics are widely used for the purpose of obtaining cosmetic effects such as whitening and improvement of rough skin. Although various substances having whitening effects are known, synthetic products often have safety problems when used on human skin for a long time, so products with good effects derived from natural ingredients are desired. [0003] Oligomeric proanthocyanidins (hereinafter also simply referred to as "proanthocyanidins" or "OPC") are a type of polyphenols t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/62A61K8/97A61K31/353A61K36/00A61K36/18A61P17/16A61Q19/00A61Q19/08B01D11/02
CPCA61K8/97A61K31/353A61K36/87C07D311/62B01D11/0288A61Q19/08A61K2236/333A61Q19/02A61K8/9789A61P17/16A61P43/00
Inventor 笠岛直树吉本祐子福井祐子
Owner SUNTORY HLDG LTD
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