Nitrobacteria and culturing method for nitrobacteria

A technology of nitrifying bacteria and culture methods, applied in the direction of bacteria, biochemical equipment and methods, and methods based on microorganisms, can solve problems such as high production costs, long industrial production cycles, and average generation time, and achieve reduced production costs and sustainable The effect of reducing aeration culture method and shortening culture time

Inactive Publication Date: 2014-08-27
HUAZHONG AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Since nitrifying bacteria are chemoautotrophic bacteria, they have the physiological characteristics of ammonia nitrogen as the only energy source, strict aerobicity, slow growth rate, and long average generation time, resulti

Method used

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  • Nitrobacteria and culturing method for nitrobacteria

Examples

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Embodiment 1

[0032] Example 1: It is the embodiment of a kind of nitrifying bacteria of the present invention, and described nitrifying bacteria, its taxonomic designation is Nitrosomonas european AT7 ( Nitrosomonas europaea AT7), the preservation date is: May 14, 2014, the preservation unit is: China Center for Type Culture Collection (CCTCC), address: Wuhan University, Luojia Mountain, Wuchang, Wuhan, and the preservation number of the preservation center is: CCTCC NO: M 2014202 The morphological and 16SrRNA gene characteristics of N. europeanum AT7 include: the scanning electron microscope of the bacteria is oval, with a length of 0.8-1.2um and a width of 0.4-0.6um; the 16S rRNA of N. The gene sequence contains sequences with greater than 98% similarity with Seq NO:1. The pH adaptation range of the nitrifying bacteria is pH=6.5-8.5; the ammonia nitrogen tolerance range is 0.1-800 mg / L, and it has a good removal effect on low-concentration ammonia nitrogen, and the low-concentration am...

Embodiment 2

[0033] Example 2: It is the basic embodiment of a kind of nitrifying bacteria culture method of the present invention: described nitrifying bacteria, its classification name is Nitrosomonas european AT7 ( Nitrosomonas europaea AT7), the nitrifying bacteria culture method comprises the following steps:

[0034] A, prepare nitrifying bacteria basal medium;

[0035] B. Preparation of nitrifying bacteria culture medium containing suspended filler:

[0036] 1) Add suspended solid adsorbents to the basal medium of nitrifying bacteria to promote the attachment and suspension growth of nitrifying bacteria;

[0037] 2) Add mixed metal salt promoter to the basal medium of nitrifying bacteria to promote the growth of nitrifying bacteria;

[0038] C. Inoculate nitrifying bacteria: Inoculate nitrifying bacteria into the nitrifying bacteria medium prepared in step B by 5% to 20% in v / v;

[0039] D. Cultivation: Cultivate the nitrifying bacteria inoculated in step C, add sodium carbonat...

Embodiment 3

[0042] Example 3: It is an example of further optimization of the nitrifying bacteria culture method based on Example 2: the amount of solid adsorbate added in the medium is 0.1% to 2% of the weight of the basic medium; the particle size of the solid adsorbate is less than 74 μm, and the density is 0.98~1.02cm 3 / g, equivalent to water, stand in water for 12 hours without complete settlement, aeration or vibration can quickly and completely suspend. The solid adsorbate is selected from straw powder, sawdust powder, corn cob powder, polycaprolactone, and is one or a mixture of two or more of them; the straw powder is rice straw, and / or wheat stalks, and / or powder made from corn stalks. The amount of the mixed metal salt promoter added in the basal medium is 0.5-10mL / L. The main components and concentrations of the mixed metal salt accelerator are calculated as w / v:

[0043] Concentration of 0.1% ~ 1% with MnCl 2 4H 2 O; Na 2 B 4 o 7 10H 2 O; ZnSO 4 ·7H 2 O; EDTA-Fe...

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Abstract

The invention relates to nitrobacteria and a culturing method for the nitrobacteria. The nitrobacteria are classified and named to be Nitrosomonas europaea AT7. The culturing method for the nitrobacteria includes the following steps: (1) preparing nitrobacteria basal culturing media, (2) preparing nitrobacteria culturing media containing suspended fillers, (3) seeding the nitrobacteria, and (4) carrying out culturing, and finally obtaining the cultured nitrobacteria. The nitrobacteria and the culturing method have the advantages that compared with the prior art, the culturing time is shortened by 30%-58%, the culturing object bacteria concentration is higher than 1*109 per milliliter and is increased by more than 10 times, intermittent aeration culturing is achieved, and the culturing cost is reduced; in addition, due to the use of the suspended adsorptive objects, when used in still water, the nitrobacteria can stay in an upper-layer water body for a long time, and the effect is better than the effect of dissociative bacteria or other adsorption materials large in density.

Description

technical field [0001] The invention relates to a nitrifying bacterium and a culture method thereof, in particular to a medium and culture conditions capable of increasing the growth rate of the nitrifying bacteria, increasing the concentration of the bacteria, and saving production costs, and is a fast and efficient culture method for the nitrifying bacteria. Background technique [0002] At present, the problem of ammonia nitrogen pollution in water body is getting more and more attention. Many application fields, such as aquaculture, eutrophic lake treatment, high ammonia nitrogen industrial wastewater treatment, etc., urgently need to remove ammonia nitrogen. [0003] There are two types of removal of ammonia nitrogen in water: physicochemical method and biological method. Physicochemical methods such as ammonia nitrogen stripping method, this method requires the use of a large amount of strong alkali, and the final ammonia nitrogen is discharged into the air, not only t...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12R1/01
Inventor 梁运祥吴定心周闯赵述淼
Owner HUAZHONG AGRI UNIV
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