Method for simultaneously detecting main components of compound Danlou tablet in plasma

A technology of plasma and tanshinone, which is applied in the field of medicine, can solve problems that have not been reported, and achieve the effect of strong specificity and good sensitivity of the method

Active Publication Date: 2014-08-27
吉林康乃尔药业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no literature reporting on the pharmacokinetics of these ingredients at the same time, and there are no reports on the basic research on the pharmacodynamic substances of Compound Danlou Tablets.

Method used

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  • Method for simultaneously detecting main components of compound Danlou tablet in plasma
  • Method for simultaneously detecting main components of compound Danlou tablet in plasma
  • Method for simultaneously detecting main components of compound Danlou tablet in plasma

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0072] Example 1. Establishment of an analysis method for the main components of Fufang Danlou Tablets in plasma samples

[0073] LC-MS / MS conditions:

[0074] The column is Agilent Eclipse Plus C 18 Column (4.6mm×100mm, 1.8μm); guard column is Agilent C18 column (2.1×12.5mm, 5μm); mobile phase: A: acetonitrile, B: water (containing 0.1% formic acid by volume fraction); flow rate is 400μL / min; column temperature is 50°C; injection volume is 5 μL; time is 0-24min;

[0075] Gradient elution was adopted, and the elution program is shown in Table 2.

[0076] Table 2 Mobile phase gradient elution program

[0077]

[0078]

[0079] Mass spectrometry conditions: using API3200 TM LC / MS / MS liquid mass analysis system; Ion source, positive and negative ion fast switching analysis mode, MRM scanning mode;

[0080] Positive ion mode: curtain gas (CUR): 30psi; collision gas (CAD): 10psi; ion spray voltage (IS): 5500V; temperature (TEM): 450°C; airflow 1 (GS1): 30psi; airflow ...

Embodiment 2

[0133] Embodiment 2, the pharmacokinetics of the main component of Compound Danlou Tablets after intragastric administration to rats

[0134] Contents of Five Compounds in Compound Danlou Tablets

[0135] LC-MS / MS was used to determine the contents of puerarin, paeoniflorin, formononetin, cryptotanshinone and tanshinone Ⅱ A in Fufangdanlan Tablets. The results showed that puerarin, paeoniflorin, formononetin and cryptotanshinone and tanshinone Ⅱ A content were 70.95mg / g, 21.07mg / g, 0.28mg / g, 1.33mg / g and 1.26mg / g.

[0136] Collection of plasma samples

[0137] The male SD rats under test were pre-adapted for one week, fasted without food and water for 12 hours the night before the test, and on the second day, the experimental animals were randomly divided into two groups, with 8 rats in each group, and 1 g·kg -1 and 6g·kg -1 Dose administered by intragastric administration, blood was collected from the fundus venous plexus at 0.083, 0.167, 0.25, 0.5, 0.75, 1, 1.5, 2, 3, 4, ...

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Abstract

The invention provides a method for simultaneously detecting main components, including puerarin, formononetin, paeoniflorin, tanshinone II A and cryptotanshinone, of a compound Danlou tablet in a plasma sample with the HPLC-MS/MS method. In a liquid chromatogram, a flow phase is composed of acetonitrile and a formic acid aqueous solution 0.1% in volume fraction, and gradient elution is adopted. A positive ion-negative ion rapidly-switching analysis mode and an MRM scanning mode are adopted for a mass spectrum. After the Danlou tablet is dosed, change conditions of the blood concentrations of the main components in the rat plasma are simultaneously detected. The methodology inspection result shows that the built method meets inner-body biological sample detecting requirements and is good in sensitivity, high in specificity, stable, reliable and suitable for detecting substance low in content.

Description

technical field [0001] The invention belongs to the field of medicine, and in particular relates to an analysis method for simultaneously detecting main components of Compound Danlou Tablets in blood plasma and its application in pharmacokinetics. Background technique [0002] Compound Danlou Tablets is a listed Chinese patent medicine composed of Gualoupi, Xiebai, Gegen, Chuanxiong, Salvia, Radix Paeoniae Rubra, Alisma, Astragalus, Rhizoma Drynariae, Curcuma, and ten traditional Chinese medicines. Clinically, it is mainly used for widening the chest and unblocking yang, dispelling phlegm and dispelling stagnation, promoting blood circulation and removing blood stasis. It is used for chest pain and heartache caused by intertwined phlegm and blood stasis. See above syndrome. It is reported in the literature that its main components are flavonoids, phenolic acids, tanshinones, triterpenes, lactones and paeoniflorin, etc., and the contents of puerarin, paeoniflorin and tanshin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88
Inventor 常艳旭吴思丹庞玉华张鹏高秀梅李晋马文芳
Owner 吉林康乃尔药业有限公司
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