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ACF detection method

A detection method and a technology of the area to be tested, which are applied in the field of detection of ACF, and can solve problems such as failure to realize

Inactive Publication Date: 2014-08-27
OLYMPUS CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there have been no reports concerning the expression changes of these molecules in the ACF lesions, and the molecules whose expression levels fluctuate are analyzed at the stage of minimal lesions represented by micro ACF (that is, the early stage) to capture the molecular biology of the future colon Rectal cancer risk and easy assessment not yet available

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Examples

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Embodiment 1

[0086] Phosphorylation of SLC2a1, SLC7a7, TRIM29, SLC2a4, CD24, ADAM17, PTGER2, CDK4 (cyclin-dependent kinase IV, cyclin-dependent kinase IV), EPHB3, C-KIT (v-kit Hardy-Zuckerman4feline sarcoma viral oncogene homolog, Proto-oncogene c-kit antibody), and GPX2, all 11 candidate molecules, compared gene expression levels in surrounding normal tissues and suspected ACF regions in the same individual, and identified gene expression levels in these candidate molecule groups that were specific in ACF up-regulated molecules.

[0087] Specifically, only samples of the area confirmed as the ACF site under the microscope were collected from the colon biopsy samples collected from 9 patients who underwent lower endoscopy, and the samples collected under the lower endoscope were prepared from the patients. For normal tissue samples, the expression levels of each molecule in each sample were measured and compared. It is shown in more detail below.

[0088] The collected large intestine mu...

Embodiment 2

[0097] According to the results of Example 1, high expression of GLUT1 was observed at both the mRNA level and the protein level in human large intestine ACF. Therefore, using the commercially available GLUT1 fluorescent probe (2-NBDG) described in Non-Patent Document 10, the probe reaction in a surgical resection specimen of human large intestine was studied.

[0098] Specifically, after spraying the GLUT1 fluorescent probe solution on the human large intestine surgical resection specimen, the fluorescence of the ACF lesion was observed under a microscope. More specifically, first, for patients diagnosed with colorectal cancer or ulcerative colitis who underwent extirpation surgery, lower endoscopy was performed before the operation, and the position of the ACF lesion was confirmed by methylene blue staining. Next, the surgical resection specimen just after the extirpation operation was washed with warm PBS, cut longitudinally, sprayed with GLUT1 fluorescent probe solution, a...

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Abstract

The present invention provides a method for detecting ACF by analyzing a test region of large intestine tissue at the molecular level. Namely, the present invention relates to a method for detecting aberrant crypt foci (ACF) that comprises detecting an ACF detection marker in a test region of large intestine tissue, by using one or more types of molecules for which ACF-specific expression increases as the ACF detection marker, the molecule being selected from the group consisting of SLC2a1, and SLC7a7; an ACF detection marker for detecting the ACF in human-derived large intestine tissue, that is SLC2a1, or SLC7a7; and, a method for evaluating risk of colorectal cancer and colorectal adenoma in subjects based on the results of detecting ACF in a test region of large intestine tissue of the subjects using the aforementioned ACF detection method.

Description

technical field [0001] The invention relates to a method for detecting ACF by using the highly expressed molecule of ACF (Aberrant Crypt Focus) as an index. [0002] this application claims priority based on Japanese Patent Application No. 2011-285215 for which it applied to Japan on December 27, 2011, and uses the content here. Background technique [0003] Colorectal cancer is the leading cause of death in Japan and the second leading cause of death from cancer in the United States. In the United States, about 150,000 people are newly diagnosed with colorectal cancer every year, and more than 50,000 people die every year (assessed by the American Cancer Society). On the other hand, since it often takes several decades for colorectal cancer to progress from a benign tumor to a malignant tumor, early risk assessment and discovery are expected to contribute to good prognosis and prevention. [0004] As the screening examination methods for colorectal adenomas and tumors com...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/02C12Q1/68G01N33/574C12N15/09
CPCG01N33/5088A61B5/0071C12Q2600/158C12Q1/6886G01N33/57419G01N33/582G01N2800/50
Inventor 小野芙美子堀野叶子高山哲治六车直树冈本耕一
Owner OLYMPUS CORP
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