Eureka AIR delivers breakthrough ideas for toughest innovation challenges, trusted by R&D personnel around the world.

Protein ST2 inhibitor polypeptide and application thereof

A protein inhibitor, 1.ST2 technology, applied in the direction of peptide/protein components, peptides, medical preparations containing active ingredients, etc., can solve problems such as drugs for uterine cancer that have not yet been developed, and achieve the effect of increasing the survival rate

Active Publication Date: 2014-09-03
国科戎安生物科技(北京)有限公司
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no drug for the treatment of uterine cancer that has developed a mature ST2 protein inhibitor polypeptide

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Protein ST2 inhibitor polypeptide and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] The effect of the ST2 protein inhibitor polypeptide on the expression of ST2 protein in the tumor was detected by the tumor model.

[0019] The human uterine cancer cell Hela tumor model was established, and the positive control drug was vincristine; the same volume of solvent was added to the blank group, and three doses were set to the experimental group: 5, 10, and 20 mg / Kg. After 21 days, they were executed, and tumor tissues were taken for ST2 protein immunoassay. Using Motic Images Advanced 3.2 image analyzer, five visual fields were continuously observed under a 200-fold lens for detection, and the gray value was measured to indicate the positive expression of ST2 protein. The smaller the gray value, the lower the positive expression of ST2 protein; the larger the gray value, the higher the positive expression of ST2 protein. As a result, ST2 protein inhibitor polypeptide 2 has the effect of inhibiting the expression of tumor ST2 protein.

[0020]

Embodiment 2

[0022] The effect of ST2 protein inhibitor polypeptide on the proliferation of human uterine cancer cell Hela.

[0023] The MTT colorimetric method was used. The logarithmic growth of Hela cells was added to a 96-well culture plate at 1.0×105, and cultured for 24 hours. Different concentrations of the experimental drug ST2 protein inhibitor polypeptide 2 and the positive control drug vincristine were added to the experimental wells and positive drug control wells, respectively; The same volume of solvent was added to the blank group. Set five duplicate wells in each well, culture for 48h, add MTT to each well at 0h, 2h, 8h, 14h, 20h, 24h, 36h, 48h respectively, after 4h of action, add DMSO, incubate for 30min, and measure at 620nm in a microplate reader Absorbance A value, according to the formula tumor cell growth inhibition rate = (1-absorbance value of the experimental group / absorbance value of the control group) × 100%. The maximum proliferation inhibition rate for Hela ...

Embodiment 3

[0026] Inhibitory test of ST2 protein inhibitor polypeptide on human cervical cancer HeLa xenograft tumor growth in nude mice

[0027] Take the human cervical cancer HeLa cell line in the logarithmic growth phase, and prepare 5 × 10 cells under sterile conditions. 7 / ml cell suspension, inoculated subcutaneously in the right armpit of nude mice with 0.1ml. Use a vernier caliper to measure the diameter of the transplanted tumor in nude mice, and wait until the tumor grows to 100-200mm 3 Animals were then randomly grouped. Using the method of measuring tumor diameter, dynamically observe the anti-tumor effect of the tested polypeptide. Tumor diameter was measured every 2 days. The method of administration was by tail vein injection. The negative control group was injected with the same amount of normal saline once a day; the paclitaxel group was given 10 mg / kg once a week; the endostar group was given 2.5 mg / kg once a day; 10mg / kg, 5mg / kg, administered once a day. After th...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the field of drugs and particularly relates to a polypeptide capable of inhibiting the expression of protein ST2 and treating uterine cancer. The sequence of the polypeptide is RNLHGLRRHTVR and is a bran-new sequence; the polypeptide can be used for inhibiting the proliferation of uterine cancer cells Hela in an in-vitro manner and treating the uterine cancer; shown by in-vivo tumor-bearing model experiments, the expression of tumor protein ST2 can be inhibited, and the survival rate of mice can be increased, so that the polypeptide has a potential value of new drug development.

Description

technical field [0001] The invention relates to ST2 protein inhibitor polypeptide and application thereof, in particular to a polypeptide capable of inhibiting ST2 protein expression and treating uterine cancer. Background technique [0002] Uterine cancer Cervical cancer is one of the most common malignant tumors in gynecology. Treatment for half a year is mainly supplemented by surgery, supplemented by radiation therapy, chemotherapy, progesterone anti-estrogen and other drugs. However, surgery is difficult to completely remove cancerous tissue and subclinical lesions. The second is radiotherapy and chemotherapy, but both of these two treatment methods have obvious side effects on normal tissues and organs. Further improving the curative effect and reducing damage to normal tissues is a topic worthy of research. At present, biological targeted therapy for tumors is very promising. Targeted therapy is aimed at blocking one or more signaling pathways in the process of tumo...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/08A61K38/10A61P35/00
Inventor 罗瑞雪
Owner 国科戎安生物科技(北京)有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com