Method for preparing basimethrin
A kind of macrobacterin and bacillus technology, applied in the field of small molecule compound preparation
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Embodiment 1
[0024] The separation method of the marine bacillus amyloliquefaciens B5 of embodiment 1 producing macrobactin:
[0025] ⑴Sample collection and enrichment Use sterile sampling bags and sterile sampling tools to collect samples of seawater, sea mud, kelp, enteromorpha, wakame, asparagus, and seaweed in Lianyungang sea area. Indicate the name of the sample, date of collection, and location of collection.
[0026] (2) The sample is processed as follows: the seawater collected from the seawater sample is mixed and 10mL is added to a 500ml Erlenmeyer flask containing 100mL of sterile seawater TSB liquid enrichment medium, and the shaker enrichment culture is carried out at 28°C and 180r / min for 2 days. . Sea mud sample collection The sea mud mixed with the five-point method is used to sample and mix again. Take 10g of sea mud and put it into a 500ml Erlenmeyer flask with 100ml of seawater TSB liquid enrichment medium sterilized in advance at 28°C, 180r / The shaker enrichment cult...
Embodiment 2
[0030] The research on the identification and phenotype of the marine Bacillus amyloliquefaciens B5 of embodiment 2 producing macrobactin:
[0031] The biological form of the above-mentioned marine bacterium B5 is described as follows: On the seawater TSB medium, the colony form: round protrusions, irregular edges, white, dry and dull, the strain’s body form: rod-shaped, with blunt round ends, Gram-positive, spores are ellipsoid, with periosteum flagella. Bacterial morphology: rod-shaped, blunt rounded ends, Gram-positive, spore-shaped, oval, with perinatal flagella, and the size is (0.5-0.8)×(1.5.-4.5) μm. Such as figure 1 , figure 2 , image 3 shown.
[0032] The physiological and biochemical indicators of the above-mentioned marine bacteria B5: the physiological and biochemical test results show that the physiological and biochemical test results of the bacterial strain B5 are consistent with the characteristics of the relevant marine bacillus amyloliquefaciens in the ...
Embodiment 3
[0048] Embodiment 3 marine bacillus amyloliquefaciens B5 produces the fermentation method of gigantin:
[0049] Strain activation: Marine Bacillus amyloliquefaciens B5 was inoculated on the seawater TSB solid slant medium by streaking method, cultured at 28°C for 18 hours, and the slant culture in logarithmic growth phase was obtained.
[0050] The configuration method of the seawater TSB solid slant medium is as follows: Weigh 17g of tryptone, 3g of soybean peptone, 2.5g of dipotassium hydrogen phosphate, 2.5g of glucose, and 5g of agar powder, and use aged seawater to heat and stir to dissolve, then add aged seawater to constant volume. 1000ml, use NaOH to adjust the pH=7.2-7.5, and then sterilize at 121°C for 15min to obtain seawater TSB solid slant medium.
[0051] Seed liquid preparation process: Under sterile conditions, pick 2-3 loops of the solid slant culture of Bacillus amyloliquefaciens B5 and inoculate it into a 500ml Erlenmeyer flask filled with 100mL sterile seaw...
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