Method for preparing GABA from yellow serofluid through fermention by microorganisms

A technology of microbial fermentation and yellow pulp water, applied in the direction of fermentation, can solve the problem of no GABA enrichment and recovery research, and achieve the effects of shortening fermentation time, improving fermentation efficiency, and simple process equipment

Inactive Publication Date: 2014-09-10
NORTHEAST AGRICULTURAL UNIVERSITY
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Problems solved by technology

All the above methods have utilized the yellow pulp water to a certain extent,

Method used

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  • Method for preparing GABA from yellow serofluid through fermention by microorganisms

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Effect test

Embodiment 1

[0018] After the soybeans are soaked, the germination temperature is 28°C for 72 hours. The germinated soybeans are refined to obtain soy milk. The soybean milk is heated, brained, and squatted to obtain yellow water. The yeast and lactic acid bacteria are separated. The compound bacteria were mixed at a compound ratio of 1:3, and the compound bacteria were inoculated into the sterilized yellow syrup water under the condition that the compound bacteria inoculation amount was 2% and the fermentation temperature was 36°C, and the fermentation broth was obtained for 20 hours. Carry out ceramic membrane ultrafiltration treatment. After ultrafiltration, the retentate and filtrate are obtained. The retentate is dehydrated by hydraulic pressure and then microwave-dried to obtain bacterial protein. The filtrate is subjected to ultrasonic treatment for 30 minutes under the conditions of ultrasonic power of 120W and ultrasonic temperature of 50℃. After ultrasonic treatment, decolorization...

Embodiment 2

[0020] After soaking the soybeans, carry out a germination treatment at a germination temperature of 26°C for 80 hours. The germinated soybeans are refined to obtain soy milk. The soybean milk is heated, brained, and squatted to obtain yellow water. The yeast and lactic acid bacteria are separated. The compound bacteria were mixed at a compound ratio of 1:2, and the compound bacteria were inoculated into the sterilized yellow syrup water under the condition of 3% compound bacteria inoculation amount and 38°C fermentation temperature to obtain fermentation broth. Perform ceramic membrane ultrafiltration treatment. After ultrafiltration, the retentate and filtrate are obtained. The retentate is dehydrated by hydraulic pressure and then microwave-dried to obtain bacterial protein. The filtrate is ultrasonicated for 40 minutes under the conditions of ultrasonic power of 100W and ultrasonic temperature of 60℃ After ultrasonic treatment, decolorization of D101 macroporous adsorption r...

Embodiment 3

[0022] After soaking the soybeans, carry out a germination treatment at a germination temperature of 30°C for 64 hours. The germinated soybeans are refined to obtain soy milk, and the soy milk is heated, brained, and squatted to obtain yellow water. The yeast and lactic acid bacteria are separated The compound bacteria were mixed in a compound ratio of 1:4, and the compound bacteria were inoculated into the sterilized yellow syrup water under the condition that the compound bacteria inoculation amount was 2% and the fermentation temperature was 34°C. The fermentation broth was obtained for 25 hours. Carry out ceramic membrane ultrafiltration treatment. After ultrafiltration, the retentate and filtrate are obtained. The retentate is dehydrated by hydraulic pressure and then microwave-dried to obtain bacterial protein. The filtrate is subjected to ultrasonic treatment for 20 minutes under the conditions of ultrasonic power of 120W and ultrasonic temperature of 40℃. After ultrasoni...

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Abstract

The invention discloses a method for preparing GABA from yellow serofluid through fermention by microorganisms, and belongs to the technical field of biological fermentation. The method comprises the following steps: (1) carrying out germination treatment on soybeans after soaking, grinding the germinated soybeans to obtain soybean milk, heating, agitating and solidifying the soybean milk, and then separating to obtain yellow serofluid; (2) inoculating and fermenting the yellow serofluid after sterilizing, so as to obtain a fermentation liquor; (3) carrying out ultrafiltration on the fermentation liquor to obtain an intercepted object and filtrate, carrying out micro-wave drying on the intercepted object to obtain mycoprotein after hydraulically pressing and dewatering, carrying out macroporous adsorption resin decolorization and ion exchange resin separation after carrying out ultrasonic treatment on the filtrate, and then carrying out vacuum concentration, cooling and crystallizing, and vacuum filtering to obtain GABA. GABA is prepared by adopting the yellow serofluid through fermentation by compound bacteria, the product is high in yield, the yield is equivalent to that of the product obtained through fermentation by adding sodium glutamate, the product purity can be over 95%, the mycoprotein also can be recovered when GABA is obtained, and industrial continuous production of GABA is facilitated.

Description

Technical field [0001] The invention belongs to the technical field of biological fermentation, and mainly relates to a method for preparing GABA by microbial fermentation of yellow pulp water. Background technique [0002] With the increasing recognition of soy products, the soy products industry has developed rapidly. During the production of soy products, a large amount of wastewater with yellow pulp water as the main body will be produced, and the total amount can reach 5.5-7 times that of bean seeds. As soy products companies are developing from small industrial scale, insufficient funds and technical strength to industrial concentration, technological upgrading, etc., if the yellow slurry water is directly discharged, it will cause serious organic pollution in the water body, so the industrialization of yellow slurry water Handling issues and comprehensive utilization issues have received widespread attention. At present, domestic research has been done on the treatment o...

Claims

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Application Information

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IPC IPC(8): C12P39/00C12P13/00
Inventor 吴非赵城彬孙楚姚子鹏周媛媛李秋刘瑶董兴叶刘金阳盛慧
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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