Method for detecting olefin components in orange juice by applying on-line solid-phase microextraction method and comprehensive two-dimensional gas chromatography-quadrupole mass spectrography

Abstract

The invention discloses a method for authenticating and analyzing olefin components in orange juice by applying an on-line solid-phase microextraction (online-SPME) method and a comprehensive two-dimensional gas chromatography-quadrupole mass spectrography (Comprehensive2DGC / qMS). The olefin components in an orange juice sample are enriched by on-line solid-phase microextraction and are detected by adopting a comprehensive two-dimensional gas chromatography-quadrupole mass spectrography; detected compounds are qualitatively analyzed in a mode of combining a retention index and an NIST spectrum library, and overlapped chromatographic peaks in the components which cannot be separated by adopting the comprehensive two-dimensional gas chromatography are accurately quantified by adopting section convolution pairs; the olefin components are subjected to orthogonal separation by adopting two chromatographic columns with different polarities by the comprehensive two-dimensional gas chromatography; under the action of a modulator-demodulator, the olefin components are detected by adopting the quadrupole mass spectrography; the qualitative analysis is performed in a mode of combining a retention time index and the NIST spectrum library; the overlapped chromatographic peaks are accurately quantified by adopting a section convolution technology. The method disclosed by the invention has the characteristics of high flux, high sensitivity and high accuracy; the problems of difficulty in analysis and separation of the olefin components in the orange juice and difficulty in qualification can be solved.

Description

technical field [0001] The invention belongs to the technical field of food safety, and relates to a method for detecting olefin components in orange juice by applying online solid-phase microextraction and comprehensive two-dimensional gas chromatography-quadrupole mass spectrometry. Background technique [0002] Orange juice is rich in nutrition and has a pleasant aroma. It is a popular and widely consumed beverage in the world. Among them, orange juice, as the main orange juice drink, ranks first in the world in consumption. In recent years, orange juice and its beverage industry have achieved great development with the improvement of people's recognition. However, due to the lack of corresponding quality control system, the phenomenon of quality adulteration, deception, and harmful substances exceeding the standard still occurs from time to time. [0003] The current national standard for the monitoring of orange juice safety mainly includes basic physical and chemical ...

AI Technical Summary

Problems solved by technology

[0005] The existing method for detecting olefins in fruit juice adopts the traditional one-dimensional gas chromatography-mass spectrometry. The separation ability of one-dimensional gas chromatography is limited, and it is impossible to completely separate all components. A single analysis may only analyze dozens of compounds. However, comprehensive two-dimensional gas chromatography-quadrupole mass spectrometry can detect hundreds of compounds, and its peak capacity is hundreds to thousands of times that of one-dimensional liquid chromatography-mass spectrometry.

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