Hydroxyapatite-silk fibroin composite reinforced calcium phosphate bone cement and preparation method thereof

A technology of calcium phosphate bone cement and hydroxyapatite, applied in the field of medicine, can solve the problems of difficult CPC mechanical strength, exothermic damage to surrounding tissues, toxic polymer biocompatibility, etc. Collapse resistance and the effect of improving mechanical properties

Inactive Publication Date: 2014-09-17
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, PMMA has many disadvantages: the monomer used for polymerization has certain toxicity and the polymer has poor biocompatibility. During the polymerization reaction, a large amount of heat will damage the surrounding tissue. At the same time, its mechanical strength is much higher than that of the surrounding natural bone, which is easy to form stress shielding.
However, due to the poor interfacial compatibility between the inorganic phase and the organic phase and the poor binding force between the two phases, it is difficult to greatly improve the mechanical strength of CPC.

Method used

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  • Hydroxyapatite-silk fibroin composite reinforced calcium phosphate bone cement and preparation method thereof
  • Hydroxyapatite-silk fibroin composite reinforced calcium phosphate bone cement and preparation method thereof
  • Hydroxyapatite-silk fibroin composite reinforced calcium phosphate bone cement and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] (1) Preparation of silk fibroin

[0033] Add silk to 0.02mol / L Na 2 CO 3 Boil in the solution for 20 minutes, and scrub with deionized water several times to remove sericin. After drying, dissolve in 9.3mol / L LiBr solution, dissolve at 60°C for 4 hours, dialyze with deionized water for 48 hours, remove silk fibroin aggregates by high-speed centrifugation, and obtain concentrations of 5wt.% and 20wt.% after concentration or dilution silk fibroin solution.

[0034] (2) Preparation of hydroxyapatite-silk fibroin composite

[0035] Weigh 7.37g Ca(OH) 2 Dissolved in 38mL water to prepare 16wt.% Ca(OH) 2 solution, after it dissolves, add 12.5mL of 20wt.% SF solution and mix well. Measure 4mLH 3 PO 4 Dilute slightly with 6mL of water, and slowly drop it into the aforementioned mixed solution while stirring at high speed, and find that the solution gradually becomes cloudy. Finally with NH 3 ·H 2 O adjust the pH to 9.0 and continue stirring for 3h. The product is fi...

Embodiment 2

[0039] (1) Preparation of silk fibroin

[0040]Add silk to 0.02mol / L Na 2 CO 3 Boil in the solution for 40 minutes, and scrub with deionized water several times to remove sericin. After drying, it was dissolved in 9.3mol / L LiBr solution, dissolved at 60°C for 4 hours, dialyzed in deionized water for 48 hours, and centrifuged at high speed to remove silk fibroin aggregates to obtain a silk fibroin solution with a concentration of 5wt.%.

[0041] (2) Preparation of hydroxyapatite-silk fibroin composite

[0042] Weigh 5.16g Ca(OH) 2 Dissolve in 30mL water to make Ca(OH) 2 solution, after it dissolves, add 60mL of 5wt.% SF solution and mix well. Measure 3.0mL H 3 PO 4 Slightly diluted with 5 mL of water, and slowly dropped into the aforementioned mixed solution while stirring at high speed, it was found that the solution gradually became cloudy. Finally with NH 3 ·H 2 O adjust the pH to 9.0 and continue stirring for 3h. The product is filtered, washed, dried and ground ...

Embodiment 3

[0046] (1) Preparation of silk fibroin

[0047] Add mulberry silk to 0.02mol / L Na 2 CO 3 Boil in the solution for 30 minutes, and scrub with deionized water several times to remove sericin. After drying, dissolve in 9.3mol / L LiBr solution, dissolve at 60°C for 4 hours, dialyze with deionized water for 48 hours, and centrifuge at high speed to remove silk fibroin aggregates, and prepare silk fibroin solutions with a concentration of 5wt.% and 10wt.%. .

[0048] (2) Preparation of hydroxyapatite-silk fibroin composite

[0049] Weigh 4.45g Ca(OH) 2 Dissolve in 25mL water to make Ca(OH) 2 solution, after it dissolves, add 40mL of 10wt.% SF solution and mix well. Measure 2.5mL H 3 PO 4 Slightly diluted with 5 mL of water, and slowly dropped into the aforementioned mixed solution while stirring at high speed, it was found that the solution gradually became cloudy. Finally with NH 3 ·H 2 O adjust the pH to 9.0 and continue stirring for 3h. The product is filtered, washed,...

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Abstract

The invention discloses a hydroxyapatite-silk fibroin (HA-SP) composite reinforced calcium phosphate bone cement and a preparation method thereof. The preparation method comprises the following steps: doping the calcium phosphate bone cement powder with 1-4wt.% of hydroxyapatite-silk fibroin composite, taking a silk fibroin solution as a blending solution and carrying out blending in a liquid-solid ratio L / P=0.33-0.4mL / g, and solidifying to obtain the hydroxyapatite-silk fibroin composite reinforced calcium phosphate bone cement. The hydroxyapatite-silk fibroin composite adopted in the preparation of the calcium phosphate bone cement is capable of effectively improving the interface compatibility of organic and inorganic phases and increasing the interface bonding force in the calcium phosphate bone cement / silk fibroin (CPC / SF) system, and thus capable of improving the mechanical properties of the calcium phosphate bone cement; the silk fibroin solution is taken as the solidifying solution so that the collapse resistance of the calcium phosphate bone cement can be effectively improved; the mechanical properties of the bone cement are improved to meet the basic requirements of clinical application.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to the most commonly used filling material in operations, in particular to a hydroxyapatite-silk fibroin reinforced calcium phosphate bone cement and a preparation method thereof. Background technique [0002] Osteoporotic or traumatic fractures are very common. In the United States, there are about 1.5 million cases of osteoporotic fractures each year, of which 750,000 cases occur in the spine, which is much higher than other diseases such as heart disease. The cost of medical care exceeded 13.8 billion US dollars in 1995, and it is expected that in 2030 it will exceed $60 billion. Vertebral Compression Fractures (Vertebral Compression Fractures, VCF) not only has a high incidence rate, but also has serious consequences. Quality of life has declined dramatically, with reported mortality rates of 34% within 5 years. [0003] The increasingly popular minimally invasive treatments...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/42
Inventor 李斌杨惠林曹呈斌李浩
Owner SUZHOU UNIV
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