Method for producing microbial fertilizer or inoculant for planting by using restaurant-kitchen garbage subjected to enzymolysis

A microbial fertilizer and kitchen waste technology, applied in the biological field, can solve the problems of high cost of use, low efficiency, and no consideration of liquid treatment and discharge of food waste, and achieve low cost, reduced viscosity, and shortened processing time. Effect

Inactive Publication Date: 2014-09-17

深圳市南理工科技有限公司

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AI-Extracted Technical Summary

Problems solved by technology

Therefore, this technology has problems such as low efficiency and high cost in practical application.

In addition, these patents do not consider the treatment and ...

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Abstract

The invention discloses a method for producing a microbial fertilizer or an inoculant for planting by using restaurant-kitchen garbage subjected to enzymolysis. The method comprises the following steps: crushing and sorting the collected restaurant-kitchen garbage to remove inorganic matters, adding high-temperature-resistant amylase during cooking and preserving heat, pouring out grease, adding glucoamylase in the cooling process and preserving heat, adding protease and cellulase and preserving heat, then performing solid-liquid separation on the restaurant-kitchen garbage subjected to enzymolysis, pumping liquid obtained by virtue of separation into a fermentation tank to perform high-temperature sterilization, inoculating microbial seed liquid for planting after cooling, fermenting for 24-48 hours at 25-37 DEG C, sub-packaging into each sealed container after fermentation to obtain the microbial fertilizer or the inoculant for liquid planting. The method disclosed by the invention aims to reduce the restaurant-kitchen garbage and perform high-valued application on the restaurant-kitchen garbage, and can be used for truly achieving restaurant-kitchen garbage treatment without secondary pollution; meanwhile, restaurant-kitchen garbage waste liquid with rich source and low cost is converted into a composite microbial fertilizer or the inoculant for planting with wide application prospects, and high-valued products are obtained; the method disclosed by the invention has particularly significant advantages.

Application Domain

Climate change adaptationOrganic fertilisers

Technology Topic

FertilizerChemistry +7

Examples

Experimental program(3)

Example Embodiment

[0017] Example 1

[0018] The collected food wastes are crushed and sorted to remove inorganic substances. When cooking at 95°C, 0.01% high temperature resistant amylase (high temperature resistant α-amylase from Bacillus licheniformis, 40000U/g, purchased from Novozymes A/ S company) and keep it for 2 hours. After the oil is poured out, add 0.03% glucoamylase (100000 U/g, purchased from Novozymes A/S company) when the temperature drops to 55℃, keep it for 2 hours; when the temperature drops to 37℃, respectively Add 0.01% protease (100000 U/g, purchased from Novozymes A/S) and 0.01% cellulase (100000 U/g, purchased from Novozymes A/S), which accounts for the weight of food waste The kitchen waste liquid is kept for 3 hours and separated from solid and liquid. The liquid is pumped into the fermentation tank. The high temperature sterilization condition is 121℃, sterilization for 25min. When the temperature is lowered to 35℃, the inoculation rate is 5% Bacillus subtilis and lichen Microbial seed liquid for planting of Bacillus, Bacillus megaterium, Bacillus natto, Bacillus coagulans and Lactobacillus plantarum (the inoculation ratio of various seed liquids is 1:1:1:1:1:1), controlled temperature 35 Cultivate for 24 hours at ℃, and finally the cell concentrations of Bacillus subtilis, Bacillus licheniformis, Bacillus megaterium, Bacillus natto, Bacillus coagulans and Lactobacillus plantarum are 28 billion cfu/mL, 22 billion cfu/mL, 150 Billion cfu/mL, 16 billion cfu/mL, 20.5 billion cfu/mL and 2.8 billion cfu/mL.

[0019] In this example, Bacillus subtilis, Bacillus licheniformis, Paenibacillus larvael, Bacillus natto and Lactobacillus plantarum were purchased from common Chinese microorganisms. The General Microbiology Center of the Preservation Management Committee is located at No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing, zip code 100101, and the preservation numbers are CGMCC No1.106, No1.941, No1.1086, No1.1630, No1.124. The preservation number of Bacillus coagulans is CGMCC No. 7646, and the preservation time is May 27, 2013 (the preservation certificate has been submitted in the invention patent application with application number 2013102643608).

[0020] The culture medium of each Bacillus seed liquid is 100 mL of water, 1.0-2.5 g of sugars, 0.1-0.3 g of yeast extract, 10 mL of bran filtered water, and pH 5.5.

[0021] The culture medium of Lactobacillus plantarum seed liquid is: peptone 1% (mass percentage), sodium chloride 0.5%, beef extract 0.3%.

Example Embodiment

[0022] Example 2

[0023] After the collected food waste is crushed and sorted to remove inorganic matter, add 0.05% high-temperature amylase (40,000 U/g, purchased from Amano Enzyme Inc.) when cooking at 90°C and keep it warm for 1-2 hours, then pour out the grease Then, add 0.05% glucoamylase (100000U/g, purchased from Novozymes A/S) when the temperature drops to 60°C, and keep it warm for 2 hours; when the temperature drops to 40°C, add 0.03% protease ( 100000U/g, purchased from Novozymes A/S company) and 0.03% cellulase (100000U/g, purchased from Novozymes A/S company), which accounts for the weight of food waste, work together with kitchen waste liquid, and solid-liquid after 3 hours of heat preservation Separate and pump the liquid into the fermentation tank. The high-temperature sterilization conditions are 115℃, sterilization for 30min, and inoculate 3% Bacillus subtilis, Bacillus licheniformis, Bacillus megaterium, Bacillus natto, Bacillus coagulans when the temperature is cooled to 35℃ Bacillus and Lactobacillus plantarum (the inoculation ratio of various seed liquids is 1:1:1:1:2:2), cultivated for 36 hours at 35℃, and finally Bacillus subtilis and Bacillus licheniformis The cell concentrations of Bacillus megaterium, Bacillus natto, Bacillus coagulans and Lactobacillus plantarum are 25.2 billion cfu/mL, 23.3 billion cfu/mL, 16.7 billion cfu/mL, 17.4 billion cfu/mL, and 36.4 billion cfu, respectively. /mL and 5 billion cfu/mL.

[0024] In this example, Bacillus subtilis, Bacillus licheniformis, Paenibacillus larvael, Bacillus natto and Lactobacillus plantarum were purchased from common Chinese microorganisms. The General Microbiology Center of the Preservation Management Committee is located at No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing, zip code 100101, and the preservation numbers are CGMCC No1.106, No1.941, No1.1086, No1.1630, No1.124. The preservation number of Bacillus coagulans is CGMCC No. 7646, and the preservation time is May 27, 2013.

[0025] The culture medium of each Bacillus seed liquid is 100 mL of water, 1.0-2.5 g of sugars, 0.1-0.3 g of yeast extract, 10 mL of bran filtered water, and pH 5.5.

[0026] The culture medium of Lactobacillus plantarum seed liquid is: peptone 1%, sodium chloride 0.5%, beef extract 0.3%.

[0027]

Example Embodiment

[0028] Example 3

[0029] After the collected food waste is crushed and sorted to remove inorganic matter, add 0.03% high-temperature resistant amylase (40,000 U/g, purchased from Amano Enzyme Inc.) when cooking at 100°C and keep it warm for 1-2 hours, then pour out the grease Then, add 0.1% glucoamylase (100000U/g, purchased from Novozymes A/S) when the temperature drops to 50°C, and keep it warm for 2 hours; but when the temperature drops to 30°C, add 0.05% protease ( 100000U/g, purchased from Amano Enzyme Inc.) and 0.05% cellulase (100000U/g, purchased from Novozymes A/S) which accounts for the weight of kitchen waste, work together with kitchen waste liquid, and solid-liquid after 5 hours of heat preservation Separate and pump the liquid into the fermentation tank. The high temperature sterilization condition is 130℃, sterilization for 20min, and when the temperature is reduced to 35℃, 1% Bacillus subtilis, Bacillus licheniformis, Bacillus megaterium, Bacillus natto, Bacillus coagulans are inoculated Bacillus and Lactobacillus plantarum are cultivated for 48 hours at a controlled temperature of 35°C. Finally, Bacillus subtilis, Bacillus licheniformis, Bacillus megaterium, Bacillus natto, Bacillus coagulans and Lactobacillus plantarum (various seeds The inoculation ratio of the solution is 1:1:2:2:1:2) The bacterial cell concentration is 22 billion cfu/mL, 20.9 billion cfu/mL, 21.2 billion cfu/mL, 22.6 billion cfu/mL, 23.1 billion cfu /mL and 5.2 billion cfu/mL.

[0030] In this example, Bacillus subtilis, Bacillus licheniformis, Paenibacillus larvael, Bacillus natto and Lactobacillus plantarum were purchased from common Chinese microorganisms. The General Microbiology Center of the Preservation Management Committee is located at No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing, zip code 100101, and the preservation numbers are CGMCC No1.106, No1.941, No1.1086, No1.1630, No1.124. The preservation number of Bacillus coagulans is CGMCC No. 7646, and the preservation time is May 27, 2013.

[0031] The culture medium of each Bacillus seed liquid is 100 mL of water, 1.0-2.5 g of sugars, 0.1-0.3 g of yeast extract, 10 mL of bran filtered water, and pH 5.5.

[0032] The culture medium of Lactobacillus plantarum seed liquid is: peptone 1%, sodium chloride 0.5%, beef extract 0.3%.

[0033]

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