Method for producing microbial fertilizer or inoculant for planting by using restaurant-kitchen garbage subjected to enzymolysis
A microbial fertilizer and kitchen waste technology, applied in the biological field, can solve the problems of high cost of use, low efficiency, and no consideration of liquid treatment and discharge of food waste, and achieve low cost, reduced viscosity, and shortened processing time. Effect
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Embodiment 1
[0018] After the collected food waste was crushed and sorted to remove inorganic substances, 0.01% high-temperature-resistant amylase (high-temperature-resistant α-amylase derived from Bacillus licheniformis, 40000U / g, purchased from Novozymes A / S company) and keep warm for 2 hours. After pouring off the oil, add 0.03% glucoamylase (100000U / g, purchased from Novozymes A / S company) when the temperature drops to 55°C, and keep warm for 2 hours; when the temperature drops to 37°C, respectively Add 0.01% protease (100000U / g, purchased from Novozymes A / S) and 0.01% cellulase (100000U / g, purchased from Novozymes A / S) by weight of food waste to act together Kitchen waste liquid, after 3 hours of heat preservation, the solid and liquid are separated, the liquid is pumped into the fermenter, the high temperature sterilization condition is 121°C, sterilized for 25min, and the inoculum is inoculated with 5% Bacillus subtilis and lichen when the temperature is lowered to 35°C Microbial s...
Embodiment 2
[0023] After the collected food waste is crushed and sorted to remove inorganic matter, add 0.05% high temperature-resistant amylase (40000U / g, purchased from Amano Enzyme Inc.) when cooking at 90°C and keep it warm for 1-2 hours, then pour off the oil Finally, when the temperature dropped to 60°C, 0.05% glucoamylase (100000U / g, purchased from Novozymes A / S Company) was added and kept for 2 hours; when the temperature dropped to 40°C, 0.03% protease ( 100000U / g, purchased from Novozymes A / S Company) and 0.03% cellulase (100000U / g, purchased from Novozymes A / S Company) of the weight of food waste together to act on the kitchen waste liquid, solid-liquid after 3 hours of incubation Separation, pump the liquid into the fermenter, high-temperature sterilization conditions are 115°C, sterilize for 30 minutes, and inoculate 3% of Bacillus subtilis, Bacillus licheniformis, Bacillus megaterium, Bacillus natto, and Bacillus coagulans when the temperature is lowered to 35°C Bacillus and...
Embodiment 3
[0029] After the collected food waste is crushed and sorted to remove inorganic matter, add 0.03% high-temperature-resistant amylase (40000U / g, purchased from Amano Enzyme Inc.) when cooking at 100°C and keep it warm for 1-2 hours, then pour off the fat Finally, when the temperature dropped to 50°C, 0.1% glucoamylase (100000U / g, purchased from Novozymes A / S Company) was added and kept for 2 hours; but when the temperature dropped to 30°C, 0.05% protease ( 100000U / g, purchased from Amano Enzyme Inc.) and 0.05% cellulase (100000U / g, purchased from Novozymes A / S Company) of the weight of food waste together acted on the kitchen waste liquid, solid-liquid after 5 hours of incubation Separation, pump the liquid into the fermenter, high-temperature sterilization conditions are 130°C, sterilize for 20 minutes, and inoculate 1% of Bacillus subtilis, Bacillus licheniformis, Bacillus megaterium, Bacillus natto, and Bacillus coagulans when the temperature is lowered to 35°C Microbial see...
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