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Method for refining Inonotus obliquus crude polysaccharide by using d301g anion resin

An anionic resin and Inonotus obliquus technology are applied in the biological field to achieve good purification effect, high yield and easy operation

Inactive Publication Date: 2016-03-30
LIAOCHENG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no literature report on the use of macroporous resin to remove pigment and protein in the crude polysaccharide of Inonotus obliquus

Method used

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  • Method for refining Inonotus obliquus crude polysaccharide by using d301g anion resin
  • Method for refining Inonotus obliquus crude polysaccharide by using d301g anion resin
  • Method for refining Inonotus obliquus crude polysaccharide by using d301g anion resin

Examples

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Effect test

Embodiment 1

[0018] Example 1 The method for refining the crude polysaccharide of Inonotus obliquus using D301G anion resin comprises the following steps:

[0019] A. Degreasing pretreatment of raw materials: Take the dried Inonotus obliquus sclerotia, crush them, soak in 4 times the volume of 60% ethanol solution for 16 hours, to remove fat, some mono (oligo) sugars and fat-soluble pigments, and place the residue in a cool place Air dry.

[0020] B. Preparation of crude polysaccharide by hot water extraction: After the pretreated residue is air-dried, add 10 times distilled water (v / w), extract in boiling water for 1 hour, filter with 80-mesh gauze, concentrate the filtrate, centrifuge, and spray-dry to obtain crude polysaccharide.

[0021] C. Crude polysaccharide enzymatic hydrolysis: dissolve the crude polysaccharide in water, prepare a 0.5% (w / w) aqueous solution, add papain, the amount of papain added is 3% (w / w) of the crude polysaccharide aqueous solution, and react at 35°C for...

Embodiment 2

[0025] Example 2 The method for refining the crude polysaccharide of Inonotus obliquus using D301G anion resin comprises the following steps:

[0026] A. Degreasing pretreatment of raw materials: Take the dried Inonotus obliquus sclerotia, crush them, soak them in 8 times the volume of 95% ethanol solution for 24 hours to remove fat, some mono (oligo) sugars and fat-soluble pigments, and place the residue in a cool place Air dry.

[0027] B. Preparation of crude polysaccharide by hot water extraction: After the pretreated residue is air-dried, add 20 times distilled water (v / w), extract with boiling water for 3 hours, filter with 120-mesh gauze, repeat the extraction of the filter residue twice, combine the filtrate, concentrate, centrifuge, and spray dry get crude polysaccharides.

[0028] C. Crude polysaccharide enzymatic hydrolysis: dissolve the crude polysaccharide in water to prepare a 2.0% (w / w) aqueous solution, add papain, the amount of papain added is 5% (w / w) o...

Embodiment 3

[0034] Example 3 The method for refining the crude polysaccharide of Inonotus obliquus using D301G anion resin comprises the following steps:

[0035] A. Degreasing pretreatment of raw materials: Take the dried Inonotus obliquus sclerotia, crush them, soak in 7 times the volume of 90% ethanol solution for 20 hours to remove fat, some mono (oligo) sugars and fat-soluble pigments, and place the residue in a cool place Air dry.

[0036] B. Preparation of crude polysaccharide by hot water leaching: After the pretreated residue is air-dried, add 18 times distilled water (v / w), extract with boiling water for 2.5 hours, filter with 100-mesh gauze, repeat the extraction of the filter residue 3 times, combine the filtrate, concentrate, centrifuge, and spray Dried crude polysaccharides.

[0037] C. Crude polysaccharide enzymatic hydrolysis: dissolve the crude polysaccharide in water to prepare a 1.8% (w / w) aqueous solution, add papain, the amount of papain added is 4.5% (w / w) of t...

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Abstract

The invention discloses a method for refining the crude polysaccharide of Inonotus obliquus by using D301G anion resin, which is characterized in that it comprises the following steps: A. Degreasing pretreatment of raw materials; B. Preparation of crude polysaccharide by hot water extraction; C. Crude polysaccharide enzymatic hydrolysis; D. Macroporous adsorption resin D301G treatment; E. Ultrafiltration to remove small molecules; F. Vacuum freeze-drying; preparation of Inonotus obliquus polysaccharide. Using this process, the decolorized and refined Inonotus obliquus polysaccharides, which remove most of the protein and small molecular impurities, are finally obtained. The polysaccharide retention rate is over 58.2%, the decolorization rate is 87.7%, and the protein removal rate is as high as 73.7%. The method is suitable for industrial scale production of the refined polysaccharide of Inonotus obliquus.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for refining crude polysaccharides from Inonotus obliquus by using D301G anion resin. Background technique [0002] Inonotus obliquus[ Inonotus obliquus (Fr.) Pilat] is a famous folk wild medicinal fungus. Studies have shown that Inonotus obliquus polysaccharide is one of the important active ingredients, which has various pharmacological activities such as enhancing immune regulation, anti-tumor, anti-oxidation, lowering blood sugar and anti-virus, and has become one of the hot research fields at home and abroad. However, there are relatively few reports on the isolation and purification of polysaccharides from Inonotus obliquus. [0003] Due to the complexity of the composition of polysaccharides, the molecular weight distribution of the crude polysaccharide extract is wide, and there are many types of polysaccharides, and the previous experiments show that t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00
Inventor 杜秀菊张扬刘珊
Owner LIAOCHENG UNIV