Extraction method of walnut green seedcase polyphenol
A technology of walnut green peel and extraction method, which is applied in the directions of pharmaceutical formulations, medical preparations containing active ingredients, antidote and the like, and achieves the effect of good application prospects.
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Embodiment 1
[0027] Example 1: Extraction of walnut green bark polyphenols
[0028] The specific method for extracting polyphenols from walnut green bark is as follows.
[0029] (1) Preparation of walnut green skin raw materials: fresh walnuts were selected as raw materials, and walnut green skin was obtained by mechanical peeling method, which were dried by hot air at 45°C, 50°C, 55°C, 60°C, and 65°C, naturally dried, and blanched. 65 ℃ hot air drying, 8 kinds of drying methods after blanching and natural drying, dry and store away from light. Determination of polyphenol content: crush the dried walnut green skin and pass through a 40-mesh sieve, use 30% ethanol, liquid-to-solid ratio 25:1 (mL / g), extraction temperature 50 ° C, extraction time 1 h to extract and extract polyphenols Determination of content, in order to investigate the influence of different drying methods on the preservation rate of polyphenols. The results were as follows: the order of polyphenol content after drying...
Embodiment 2
[0040] Example 2: Single factor test of walnut green bark polyphenol extraction
[0041] Based on the above examples, the walnut green peel polyphenol extraction method adopted is to select fresh walnuts as raw materials, and adopt mechanical peeling method to obtain walnut green peel, which is naturally dried after blanching, crushed and passed through a 40-mesh sieve, and dried Crushed and sieved walnut green skin powder Proportion Add pure water, place in ultrasonic extraction equipment, and undergo an ultrasonic-assisted extraction process. The specific process parameters are verified by a single factor test as follows.
[0042] With a fixed single ultrasonic time of 5s, different stirring speeds of 0r / min, 400r / min, 600r / min, 800r / min, 1200r / min, 1600r / min, and different stirring speeds of 200W, 400W, 600W, 800W, 1000W Ultrasonic power, different ultrasonic gap time of 1s, 3s, 5s, 7s, 9s, different ultrasonic full time of 10min, 15min, 20min, 25min, 30min, 40℃, 50℃, 6...
Embodiment 3
[0051] Example 3: Response Surface Test of Walnut Green Bark Polyphenols
[0052] It can be seen from the above-mentioned single factor test of Example 2 that the three factors of ultrasonic power, heating temperature, and liquid-material ratio have a more significant impact on the yield of polyphenols. Therefore, the three factors of ultrasonic power, heating temperature, and liquid-material ratio were selected, and the Box-behnken test was carried out with the yield of polyphenols as the response value, as shown in Table 1. Data analysis was performed using Design Expert8.0.5.b software to optimize the ultrasonic Process parameters for auxiliary extraction of polyphenols from walnut green bark.
[0053] Table 1 Response surface factor level coding list
[0054]
[0055] 1.1 Analysis of response surface results:
[0056] Table 2 Results of Box-benhnken experimental design
[0057]
[0058] Table 3 Analysis of variance of response surface quadratic model
[0059] ...
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